Urucuri virus
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S5SHX6|A0A1S5SHX6_9VIRU Nonstructural protein OS=Urucuri virus OX=1926502 PE=4 SV=1
MM1 pKa = 7.45 NRR3 pKa = 11.84 FLRR6 pKa = 11.84 DD7 pKa = 3.07 RR8 pKa = 11.84 PYY10 pKa = 10.94 VSQSFLFLGKK20 pKa = 9.15 PQVDD24 pKa = 3.87 YY25 pKa = 11.42 IPFNKK30 pKa = 8.95 VWCEE34 pKa = 3.46 PVSAHH39 pKa = 6.18 NGMEE43 pKa = 4.59 FIVNHH48 pKa = 5.23 YY49 pKa = 9.92 RR50 pKa = 11.84 QVADD54 pKa = 3.71 CRR56 pKa = 11.84 PRR58 pKa = 11.84 LDD60 pKa = 4.07 DD61 pKa = 4.11 FYY63 pKa = 11.65 SRR65 pKa = 11.84 RR66 pKa = 11.84 EE67 pKa = 4.04 LPVTWGPFDD76 pKa = 4.68 GSVQNEE82 pKa = 4.22 STDD85 pKa = 3.35 QFLSLIHH92 pKa = 6.42 EE93 pKa = 4.57 LSKK96 pKa = 11.31 LDD98 pKa = 3.42 ILDD101 pKa = 3.7 ANRR104 pKa = 11.84 YY105 pKa = 8.83 NEE107 pKa = 4.04 PSLRR111 pKa = 11.84 RR112 pKa = 11.84 ALRR115 pKa = 11.84 WPFTYY120 pKa = 8.54 PTDD123 pKa = 3.93 QFFKK127 pKa = 10.79 LAADD131 pKa = 4.52 RR132 pKa = 11.84 EE133 pKa = 4.67 NIGPWSWKK141 pKa = 9.48 NFAMTCIIRR150 pKa = 11.84 SVNEE154 pKa = 3.73 MNLDD158 pKa = 3.4 KK159 pKa = 11.2 AVVSFHH165 pKa = 6.58 KK166 pKa = 10.56 KK167 pKa = 9.18 ILAEE171 pKa = 4.01 VQLQGGRR178 pKa = 11.84 ISEE181 pKa = 4.2 FPGRR185 pKa = 11.84 DD186 pKa = 3.66 LILEE190 pKa = 4.38 AASVQCMRR198 pKa = 11.84 LVNAYY203 pKa = 9.44 KK204 pKa = 10.51 ADD206 pKa = 3.72 ADD208 pKa = 3.57 KK209 pKa = 10.86 TPRR212 pKa = 11.84 HH213 pKa = 5.07 SRR215 pKa = 11.84 YY216 pKa = 8.98 YY217 pKa = 10.74 LAVKK221 pKa = 10.25 DD222 pKa = 3.76 YY223 pKa = 11.68 DD224 pKa = 4.21 EE225 pKa = 5.16 NCLHH229 pKa = 7.22 NDD231 pKa = 3.82 RR232 pKa = 11.84 LSKK235 pKa = 8.29 WTQDD239 pKa = 2.73 TDD241 pKa = 4.24 DD242 pKa = 4.5 YY243 pKa = 11.86 VDD245 pKa = 3.99 MDD247 pKa = 4.41 YY248 pKa = 11.37 LSQALEE254 pKa = 4.28 SEE256 pKa = 4.13 WTIDD260 pKa = 3.74 EE261 pKa = 4.82 SEE263 pKa = 4.38 TEE265 pKa = 3.93
Molecular weight: 31.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.069
IPC2_protein 5.143
IPC_protein 5.092
Toseland 5.041
ProMoST 5.169
Dawson 5.105
Bjellqvist 5.258
Wikipedia 5.016
Rodwell 5.016
Grimsley 4.978
Solomon 5.105
Lehninger 5.067
Nozaki 5.245
DTASelect 5.436
Thurlkill 5.067
EMBOSS 5.067
Sillero 5.308
Patrickios 3.986
IPC_peptide 5.118
IPC2_peptide 5.296
IPC2.peptide.svr19 5.292
Protein with the highest isoelectric point:
>tr|A0A1S5SHX6|A0A1S5SHX6_9VIRU Nonstructural protein OS=Urucuri virus OX=1926502 PE=4 SV=1
MM1 pKa = 7.95 ADD3 pKa = 3.58 YY4 pKa = 10.82 QRR6 pKa = 11.84 LAVEE10 pKa = 5.09 FASEE14 pKa = 4.5 VPSEE18 pKa = 4.3 TVVRR22 pKa = 11.84 QWVEE26 pKa = 3.6 DD27 pKa = 3.87 FAYY30 pKa = 10.48 QGFDD34 pKa = 3.13 ANNVMRR40 pKa = 11.84 LLVEE44 pKa = 4.23 RR45 pKa = 11.84 GGNSWKK51 pKa = 10.64 DD52 pKa = 3.6 DD53 pKa = 3.52 ARR55 pKa = 11.84 KK56 pKa = 9.22 MIVLCLTRR64 pKa = 11.84 GNKK67 pKa = 7.62 PNKK70 pKa = 9.12 MIEE73 pKa = 4.16 KK74 pKa = 9.54 MSEE77 pKa = 3.68 TGKK80 pKa = 9.19 ATVKK84 pKa = 10.55 QLIAKK89 pKa = 9.77 YY90 pKa = 8.95 SLRR93 pKa = 11.84 TGNPGRR99 pKa = 11.84 NEE101 pKa = 3.81 LTLSRR106 pKa = 11.84 VCTALAGWTCQAAVVVQDD124 pKa = 3.73 YY125 pKa = 11.2 LPVTGRR131 pKa = 11.84 TMDD134 pKa = 3.7 NYY136 pKa = 10.11 SANYY140 pKa = 7.17 PRR142 pKa = 11.84 PMMHH146 pKa = 7.22 PSFAGLIDD154 pKa = 3.57 RR155 pKa = 11.84 TLEE158 pKa = 4.08 DD159 pKa = 3.25 SVMEE163 pKa = 4.69 EE164 pKa = 4.21 IVDD167 pKa = 3.54 AHH169 pKa = 6.76 CLFMVEE175 pKa = 4.61 FSKK178 pKa = 10.02 TINPNTRR185 pKa = 11.84 SLSSAEE191 pKa = 3.93 VLKK194 pKa = 10.03 TFQQPMRR201 pKa = 11.84 AAINSNFLTSAQRR214 pKa = 11.84 RR215 pKa = 11.84 GFLIEE220 pKa = 4.47 LGIINEE226 pKa = 4.02 NLKK229 pKa = 10.53 VSPAVADD236 pKa = 3.52 AAKK239 pKa = 10.38 KK240 pKa = 9.87 FRR242 pKa = 11.84 HH243 pKa = 4.69 QTRR246 pKa = 11.84 II247 pKa = 3.31
Molecular weight: 27.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.284
IPC2_protein 8.214
IPC_protein 8.2
Toseland 8.565
ProMoST 8.653
Dawson 8.99
Bjellqvist 9.048
Wikipedia 9.033
Rodwell 9.077
Grimsley 8.521
Solomon 9.165
Lehninger 9.151
Nozaki 9.224
DTASelect 8.873
Thurlkill 8.975
EMBOSS 9.165
Sillero 9.209
Patrickios 4.838
IPC_peptide 9.151
IPC2_peptide 7.863
IPC2.peptide.svr19 7.741
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3984
247
2120
996.0
112.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.146 ± 0.914
2.56 ± 0.676
5.949 ± 0.691
6.928 ± 0.347
4.192 ± 0.468
5.522 ± 0.473
2.209 ± 0.229
6.476 ± 0.659
6.576 ± 0.577
8.208 ± 0.273
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.338 ± 0.454
4.267 ± 0.591
3.815 ± 0.197
3.338 ± 0.236
5.673 ± 0.536
9.513 ± 0.954
5.346 ± 0.321
6.627 ± 0.403
1.381 ± 0.187
2.937 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here