Urucuri virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Urucuri phlebovirus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1S5SHX6|A0A1S5SHX6_9VIRU Nonstructural protein OS=Urucuri virus OX=1926502 PE=4 SV=1
MM1 pKa = 7.45NRR3 pKa = 11.84FLRR6 pKa = 11.84DD7 pKa = 3.07RR8 pKa = 11.84PYY10 pKa = 10.94VSQSFLFLGKK20 pKa = 9.15PQVDD24 pKa = 3.87YY25 pKa = 11.42IPFNKK30 pKa = 8.95VWCEE34 pKa = 3.46PVSAHH39 pKa = 6.18NGMEE43 pKa = 4.59FIVNHH48 pKa = 5.23YY49 pKa = 9.92RR50 pKa = 11.84QVADD54 pKa = 3.71CRR56 pKa = 11.84PRR58 pKa = 11.84LDD60 pKa = 4.07DD61 pKa = 4.11FYY63 pKa = 11.65SRR65 pKa = 11.84RR66 pKa = 11.84EE67 pKa = 4.04LPVTWGPFDD76 pKa = 4.68GSVQNEE82 pKa = 4.22STDD85 pKa = 3.35QFLSLIHH92 pKa = 6.42EE93 pKa = 4.57LSKK96 pKa = 11.31LDD98 pKa = 3.42ILDD101 pKa = 3.7ANRR104 pKa = 11.84YY105 pKa = 8.83NEE107 pKa = 4.04PSLRR111 pKa = 11.84RR112 pKa = 11.84ALRR115 pKa = 11.84WPFTYY120 pKa = 8.54PTDD123 pKa = 3.93QFFKK127 pKa = 10.79LAADD131 pKa = 4.52RR132 pKa = 11.84EE133 pKa = 4.67NIGPWSWKK141 pKa = 9.48NFAMTCIIRR150 pKa = 11.84SVNEE154 pKa = 3.73MNLDD158 pKa = 3.4KK159 pKa = 11.2AVVSFHH165 pKa = 6.58KK166 pKa = 10.56KK167 pKa = 9.18ILAEE171 pKa = 4.01VQLQGGRR178 pKa = 11.84ISEE181 pKa = 4.2FPGRR185 pKa = 11.84DD186 pKa = 3.66LILEE190 pKa = 4.38AASVQCMRR198 pKa = 11.84LVNAYY203 pKa = 9.44KK204 pKa = 10.51ADD206 pKa = 3.72ADD208 pKa = 3.57KK209 pKa = 10.86TPRR212 pKa = 11.84HH213 pKa = 5.07SRR215 pKa = 11.84YY216 pKa = 8.98YY217 pKa = 10.74LAVKK221 pKa = 10.25DD222 pKa = 3.76YY223 pKa = 11.68DD224 pKa = 4.21EE225 pKa = 5.16NCLHH229 pKa = 7.22NDD231 pKa = 3.82RR232 pKa = 11.84LSKK235 pKa = 8.29WTQDD239 pKa = 2.73TDD241 pKa = 4.24DD242 pKa = 4.5YY243 pKa = 11.86VDD245 pKa = 3.99MDD247 pKa = 4.41YY248 pKa = 11.37LSQALEE254 pKa = 4.28SEE256 pKa = 4.13WTIDD260 pKa = 3.74EE261 pKa = 4.82SEE263 pKa = 4.38TEE265 pKa = 3.93

Molecular weight:
31.16 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1S5SHX6|A0A1S5SHX6_9VIRU Nonstructural protein OS=Urucuri virus OX=1926502 PE=4 SV=1
MM1 pKa = 7.95ADD3 pKa = 3.58YY4 pKa = 10.82QRR6 pKa = 11.84LAVEE10 pKa = 5.09FASEE14 pKa = 4.5VPSEE18 pKa = 4.3TVVRR22 pKa = 11.84QWVEE26 pKa = 3.6DD27 pKa = 3.87FAYY30 pKa = 10.48QGFDD34 pKa = 3.13ANNVMRR40 pKa = 11.84LLVEE44 pKa = 4.23RR45 pKa = 11.84GGNSWKK51 pKa = 10.64DD52 pKa = 3.6DD53 pKa = 3.52ARR55 pKa = 11.84KK56 pKa = 9.22MIVLCLTRR64 pKa = 11.84GNKK67 pKa = 7.62PNKK70 pKa = 9.12MIEE73 pKa = 4.16KK74 pKa = 9.54MSEE77 pKa = 3.68TGKK80 pKa = 9.19ATVKK84 pKa = 10.55QLIAKK89 pKa = 9.77YY90 pKa = 8.95SLRR93 pKa = 11.84TGNPGRR99 pKa = 11.84NEE101 pKa = 3.81LTLSRR106 pKa = 11.84VCTALAGWTCQAAVVVQDD124 pKa = 3.73YY125 pKa = 11.2LPVTGRR131 pKa = 11.84TMDD134 pKa = 3.7NYY136 pKa = 10.11SANYY140 pKa = 7.17PRR142 pKa = 11.84PMMHH146 pKa = 7.22PSFAGLIDD154 pKa = 3.57RR155 pKa = 11.84TLEE158 pKa = 4.08DD159 pKa = 3.25SVMEE163 pKa = 4.69EE164 pKa = 4.21IVDD167 pKa = 3.54AHH169 pKa = 6.76CLFMVEE175 pKa = 4.61FSKK178 pKa = 10.02TINPNTRR185 pKa = 11.84SLSSAEE191 pKa = 3.93VLKK194 pKa = 10.03TFQQPMRR201 pKa = 11.84AAINSNFLTSAQRR214 pKa = 11.84RR215 pKa = 11.84GFLIEE220 pKa = 4.47LGIINEE226 pKa = 4.02NLKK229 pKa = 10.53VSPAVADD236 pKa = 3.52AAKK239 pKa = 10.38KK240 pKa = 9.87FRR242 pKa = 11.84HH243 pKa = 4.69QTRR246 pKa = 11.84II247 pKa = 3.31

Molecular weight:
27.76 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3984

247

2120

996.0

112.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.146 ± 0.914

2.56 ± 0.676

5.949 ± 0.691

6.928 ± 0.347

4.192 ± 0.468

5.522 ± 0.473

2.209 ± 0.229

6.476 ± 0.659

6.576 ± 0.577

8.208 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.338 ± 0.454

4.267 ± 0.591

3.815 ± 0.197

3.338 ± 0.236

5.673 ± 0.536

9.513 ± 0.954

5.346 ± 0.321

6.627 ± 0.403

1.381 ± 0.187

2.937 ± 0.224

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski