Dragonfly larvae associated circular virus-8

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5U2Q5|W5U2Q5_9VIRU Replication-associated protein OS=Dragonfly larvae associated circular virus-8 OX=1454029 PE=4 SV=1
MM1 pKa = 7.78ANPNTCRR8 pKa = 11.84WMITLFGEE16 pKa = 4.53EE17 pKa = 4.75DD18 pKa = 3.65PVMPLPVAYY27 pKa = 9.86GCWGKK32 pKa = 7.78EE33 pKa = 3.9TCPTTGRR40 pKa = 11.84VHH42 pKa = 5.96SHH44 pKa = 5.59MYY46 pKa = 10.53LRR48 pKa = 11.84FDD50 pKa = 3.66KK51 pKa = 10.26KK52 pKa = 11.05CRR54 pKa = 11.84MSTLKK59 pKa = 10.53NRR61 pKa = 11.84LQRR64 pKa = 11.84DD65 pKa = 3.69DD66 pKa = 3.96AHH68 pKa = 7.04CEE70 pKa = 3.44PAAGKK75 pKa = 9.07EE76 pKa = 4.1QACRR80 pKa = 11.84DD81 pKa = 4.07YY82 pKa = 10.94CWSEE86 pKa = 3.61GDD88 pKa = 3.97HH89 pKa = 7.02ADD91 pKa = 3.74KK92 pKa = 11.01LPHH95 pKa = 6.65RR96 pKa = 11.84LVKK99 pKa = 10.63GPEE102 pKa = 3.66IGEE105 pKa = 4.25FQADD109 pKa = 3.24MGQGKK114 pKa = 10.08RR115 pKa = 11.84SDD117 pKa = 3.91LDD119 pKa = 4.64AIADD123 pKa = 3.81DD124 pKa = 4.16VKK126 pKa = 10.74RR127 pKa = 11.84GSSEE131 pKa = 3.71RR132 pKa = 11.84QIAEE136 pKa = 4.29KK137 pKa = 10.6YY138 pKa = 9.13SSDD141 pKa = 4.22FIRR144 pKa = 11.84YY145 pKa = 8.51SAGIRR150 pKa = 11.84NFIAVVAPKK159 pKa = 10.2PPLQRR164 pKa = 11.84IVLVTLLWGPTGTGKK179 pKa = 7.33THH181 pKa = 6.87RR182 pKa = 11.84CFTAFPEE189 pKa = 4.77LYY191 pKa = 9.68SVKK194 pKa = 10.1PGRR197 pKa = 11.84DD198 pKa = 2.74PWGLYY203 pKa = 9.78RR204 pKa = 11.84GEE206 pKa = 4.16GALANAPSPPDD217 pKa = 3.61TPHH220 pKa = 6.53PQHH223 pKa = 6.16TLSTGSLTSRR233 pKa = 11.84TMISIVNTLLIFYY246 pKa = 9.9CSMFHH251 pKa = 6.72VPKK254 pKa = 10.75CSS256 pKa = 2.98

Molecular weight: 28.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5U2Q5|W5U2Q5_9VIRU Replication-associated protein OS=Dragonfly larvae associated circular virus-8 OX=1454029 PE=4 SV=1
MM1 pKa = 7.2KK2 pKa = 10.28RR3 pKa = 11.84IARR6 pKa = 11.84RR7 pKa = 11.84VVNSAIEE14 pKa = 4.15TKK16 pKa = 9.97MIEE19 pKa = 4.06ASLGPFGSVTTTPSTGFFAPAAGTARR45 pKa = 11.84DD46 pKa = 3.64QRR48 pKa = 11.84VGRR51 pKa = 11.84VITPTYY57 pKa = 10.13FKK59 pKa = 11.01LRR61 pKa = 11.84GLLHH65 pKa = 6.69GAQSNVAVDD74 pKa = 3.8DD75 pKa = 3.53SHH77 pKa = 8.72NIFRR81 pKa = 11.84ILLLVTDD88 pKa = 4.43YY89 pKa = 11.44DD90 pKa = 3.62WTYY93 pKa = 9.53GTSIATNNYY102 pKa = 7.81IDD104 pKa = 4.07PGKK107 pKa = 10.53GVHH110 pKa = 6.52HH111 pKa = 7.33KK112 pKa = 10.12IKK114 pKa = 10.38QVLYY118 pKa = 10.04DD119 pKa = 3.71RR120 pKa = 11.84TMSLCSPGRR129 pKa = 11.84DD130 pKa = 3.27STGYY134 pKa = 10.41LAVQKK139 pKa = 9.8EE140 pKa = 4.28INIKK144 pKa = 10.32ASLRR148 pKa = 11.84RR149 pKa = 11.84MGKK152 pKa = 8.06MVWDD156 pKa = 4.34DD157 pKa = 3.5SGNPSKK163 pKa = 10.87LLYY166 pKa = 10.62LVMVSDD172 pKa = 4.52SVISVNPGFTDD183 pKa = 4.04GQYY186 pKa = 10.81RR187 pKa = 11.84FYY189 pKa = 11.41YY190 pKa = 10.31KK191 pKa = 10.69DD192 pKa = 3.07GG193 pKa = 3.84

Molecular weight: 21.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

449

193

256

224.5

25.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.682 ± 0.301

2.45 ± 1.255

6.236 ± 0.325

3.341 ± 1.16

3.563 ± 0.041

8.241 ± 0.369

2.895 ± 0.534

5.122 ± 0.711

5.568 ± 0.085

7.35 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.118 ± 0.006

3.118 ± 0.667

6.459 ± 1.503

2.673 ± 0.053

6.904 ± 0.109

7.35 ± 0.611

7.127 ± 0.419

6.236 ± 1.334

1.559 ± 0.34

4.009 ± 0.761
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski