Capsicum baccatum (Peruvian pepper)
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 35641 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2G2V1C7|A0A2G2V1C7_CAPBA Serine/threonine-protein kinase TOR OS=Capsicum baccatum OX=33114 GN=CQW23_33612 PE=4 SV=1
MM1 pKa = 7.08 NGEE4 pKa = 4.11 VRR6 pKa = 11.84 WYY8 pKa = 9.31 VSSRR12 pKa = 11.84 FSFTLSLVGLVFIIDD27 pKa = 4.97 CITYY31 pKa = 8.67 ICVDD35 pKa = 3.2 GVMVLDD41 pKa = 4.02 EE42 pKa = 4.52 FEE44 pKa = 5.0 GYY46 pKa = 10.17 INFMMMPDD54 pKa = 3.57 KK55 pKa = 11.14 SRR57 pKa = 11.84 GYY59 pKa = 8.92 MDD61 pKa = 5.71 DD62 pKa = 2.92 MMMPGDD68 pKa = 3.82 SKK70 pKa = 11.5 GYY72 pKa = 7.76 TDD74 pKa = 5.23 VMMVPNLFGGYY85 pKa = 9.14 NDD87 pKa = 5.33 DD88 pKa = 5.45 MMMPDD93 pKa = 3.44 NFVGYY98 pKa = 10.22 VDD100 pKa = 6.49 DD101 pKa = 5.73 IMLPGNSKK109 pKa = 10.88 GYY111 pKa = 9.76 IDD113 pKa = 6.05 DD114 pKa = 4.17 VMIPDD119 pKa = 3.81 KK120 pKa = 11.1 FKK122 pKa = 11.06 GYY124 pKa = 9.09 TDD126 pKa = 5.02 DD127 pKa = 5.77 IYY129 pKa = 11.57 VVWDD133 pKa = 3.61 AVMDD137 pKa = 3.93 SLLYY141 pKa = 10.65 VLLGVLPDD149 pKa = 3.97 GMGHH153 pKa = 6.96 RR154 pKa = 11.84 GNFGQRR160 pKa = 11.84 AIQPKK165 pKa = 9.76
Molecular weight: 18.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.923
IPC_protein 3.935
Toseland 3.694
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.948
Rodwell 3.757
Grimsley 3.605
Solomon 3.948
Lehninger 3.91
Nozaki 4.075
DTASelect 4.406
Thurlkill 3.77
EMBOSS 3.948
Sillero 4.062
Patrickios 1.1
IPC_peptide 3.935
IPC2_peptide 4.037
IPC2.peptide.svr19 3.936
Protein with the highest isoelectric point:
>tr|A0A2G2V2L8|A0A2G2V2L8_CAPBA Ethylene-responsive transcription factor 4 OS=Capsicum baccatum OX=33114 GN=CQW23_33169 PE=4 SV=1
MM1 pKa = 7.65 APGHH5 pKa = 6.79 HH6 pKa = 6.09 GTKK9 pKa = 10.36 APWRR13 pKa = 11.84 HH14 pKa = 4.83 ATVAPRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GTMASGHH29 pKa = 6.54 KK30 pKa = 9.1 GTMTARR36 pKa = 11.84 HH37 pKa = 5.91 QGTVAPWHH45 pKa = 6.82 RR46 pKa = 11.84 GTKK49 pKa = 9.38 AAWHH53 pKa = 6.6 HH54 pKa = 5.22 RR55 pKa = 11.84 TKK57 pKa = 11.05 APWHH61 pKa = 4.92 QVGVQPWHH69 pKa = 6.38 QGTMPPRR76 pKa = 11.84 HH77 pKa = 6.81 HH78 pKa = 7.17 DD79 pKa = 3.24 TVAPRR84 pKa = 11.84 RR85 pKa = 11.84 PCTLAPRR92 pKa = 11.84 HH93 pKa = 6.28 RR94 pKa = 11.84 GTKK97 pKa = 8.84 VAWHH101 pKa = 7.05 HH102 pKa = 5.45 GTVAPWHH109 pKa = 5.73 QGTRR113 pKa = 11.84 AAWHH117 pKa = 6.66 HH118 pKa = 5.93 GAMAPGHH125 pKa = 6.09 QGTVAPRR132 pKa = 11.84 WRR134 pKa = 11.84 GTMAHH139 pKa = 6.28 RR140 pKa = 11.84 HH141 pKa = 5.91 HH142 pKa = 6.59 GTVAPWNPGTMAPWHH157 pKa = 6.76 HH158 pKa = 6.14 GTNASRR164 pKa = 11.84 HH165 pKa = 5.12 QGTVTPWHH173 pKa = 7.02 HH174 pKa = 5.61 GTVAPWHH181 pKa = 6.44 RR182 pKa = 11.84 GTRR185 pKa = 11.84 ALRR188 pKa = 11.84 HH189 pKa = 6.31 HH190 pKa = 6.42 GTMAPWHH197 pKa = 7.0 HH198 pKa = 5.86 GTMAPRR204 pKa = 11.84 HH205 pKa = 6.08 RR206 pKa = 11.84 GLVAAA211 pKa = 5.66
Molecular weight: 23.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.476
IPC2_protein 11.038
IPC_protein 12.647
Toseland 12.808
ProMoST 13.305
Dawson 12.808
Bjellqvist 12.808
Wikipedia 13.276
Rodwell 12.398
Grimsley 12.852
Solomon 13.305
Lehninger 13.203
Nozaki 12.808
DTASelect 12.808
Thurlkill 12.808
EMBOSS 13.305
Sillero 12.808
Patrickios 12.12
IPC_peptide 13.305
IPC2_peptide 12.296
IPC2.peptide.svr19 9.133
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
35641
0
35641
13376091
28
5007
375.3
42.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.355 ± 0.012
1.907 ± 0.007
5.398 ± 0.011
6.437 ± 0.013
4.201 ± 0.008
6.369 ± 0.012
2.387 ± 0.005
5.587 ± 0.01
6.303 ± 0.011
9.798 ± 0.015
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.497 ± 0.006
4.644 ± 0.009
4.837 ± 0.015
3.555 ± 0.008
5.232 ± 0.011
8.777 ± 0.014
4.93 ± 0.008
6.554 ± 0.01
1.293 ± 0.005
2.925 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here