Streptococcus satellite phage Javan376

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZL86|A0A4D5ZL86_9VIRU Virulence-associated protein E OS=Streptococcus satellite phage Javan376 OX=2558667 GN=JavanS376_0003 PE=4 SV=1
MM1 pKa = 7.68EE2 pKa = 5.44IKK4 pKa = 10.03DD5 pKa = 3.38AKK7 pKa = 10.31KK8 pKa = 10.03IYY10 pKa = 10.33FEE12 pKa = 4.91LVQNYY17 pKa = 9.94NLFNKK22 pKa = 9.54KK23 pKa = 7.51STYY26 pKa = 10.38FGVEE30 pKa = 3.67NNDD33 pKa = 3.24NYY35 pKa = 11.18HH36 pKa = 5.78YY37 pKa = 10.86LSLGLIPEE45 pKa = 4.42IASTLSGGKK54 pKa = 9.8EE55 pKa = 3.31IIKK58 pKa = 9.91FVEE61 pKa = 5.1EE62 pKa = 3.84ICSSIKK68 pKa = 10.51KK69 pKa = 9.29YY70 pKa = 10.11WEE72 pKa = 4.02LRR74 pKa = 11.84TKK76 pKa = 10.56SIEE79 pKa = 4.13EE80 pKa = 3.86MDD82 pKa = 3.82KK83 pKa = 11.15LLSDD87 pKa = 4.99RR88 pKa = 11.84YY89 pKa = 9.1LTSKK93 pKa = 10.79KK94 pKa = 9.72KK95 pKa = 9.92DD96 pKa = 3.38QKK98 pKa = 10.14ATEE101 pKa = 4.13LKK103 pKa = 10.78KK104 pKa = 10.94EE105 pKa = 3.56ITLNLNEE112 pKa = 4.15LVKK115 pKa = 11.14VNTKK119 pKa = 9.99LASKK123 pKa = 8.79QEE125 pKa = 4.3KK126 pKa = 9.92VFSPILKK133 pKa = 9.25IVKK136 pKa = 9.1EE137 pKa = 3.86ASEE140 pKa = 4.06ALGEE144 pKa = 4.18FGDD147 pKa = 4.42NKK149 pKa = 10.25VLPSKK154 pKa = 10.49IDD156 pKa = 3.39LYY158 pKa = 10.74TNHH161 pKa = 7.46PEE163 pKa = 3.84CTEE166 pKa = 3.97IEE168 pKa = 4.23FTNYY172 pKa = 9.38FVDD175 pKa = 3.99EE176 pKa = 5.52LYY178 pKa = 10.51TPYY181 pKa = 10.52PPLKK185 pKa = 10.78DD186 pKa = 3.39RR187 pKa = 11.84DD188 pKa = 3.63FNYY191 pKa = 10.31FIRR194 pKa = 11.84IGEE197 pKa = 4.12EE198 pKa = 4.05VSFTRR203 pKa = 11.84HH204 pKa = 5.33AEE206 pKa = 4.02AEE208 pKa = 4.09FEE210 pKa = 4.28EE211 pKa = 5.47LGLPTLNRR219 pKa = 11.84HH220 pKa = 4.86LTNFKK225 pKa = 10.69VEE227 pKa = 4.45NYY229 pKa = 8.08WKK231 pKa = 10.65EE232 pKa = 3.79NGFSSKK238 pKa = 10.52VEE240 pKa = 3.83WLASVHH246 pKa = 5.69EE247 pKa = 4.26KK248 pKa = 10.48RR249 pKa = 11.84KK250 pKa = 9.16EE251 pKa = 4.0AEE253 pKa = 3.74EE254 pKa = 4.19LEE256 pKa = 4.52YY257 pKa = 10.64IEE259 pKa = 5.3DD260 pKa = 3.48MKK262 pKa = 10.62MQQALKK268 pKa = 9.91EE269 pKa = 4.09LKK271 pKa = 10.56AQGKK275 pKa = 8.75QEE277 pKa = 4.05GFFKK281 pKa = 10.86KK282 pKa = 10.01MLGAFTNEE290 pKa = 3.69

Molecular weight: 33.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZRB6|A0A4D5ZRB6_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan376 OX=2558667 GN=JavanS376_0007 PE=3 SV=1
MM1 pKa = 7.54NIKK4 pKa = 8.96TVVKK8 pKa = 10.72KK9 pKa = 10.62NGQTVYY15 pKa = 10.41RR16 pKa = 11.84ASVYY20 pKa = 10.77LGVDD24 pKa = 2.88QMTGKK29 pKa = 10.56KK30 pKa = 10.11SRR32 pKa = 11.84TTVTANTKK40 pKa = 8.69TAVKK44 pKa = 9.95IKK46 pKa = 10.44ARR48 pKa = 11.84EE49 pKa = 4.34AINTFANNGYY59 pKa = 8.85CAKK62 pKa = 10.05NKK64 pKa = 8.48PTITTYY70 pKa = 10.95RR71 pKa = 11.84EE72 pKa = 4.14LVALWWEE79 pKa = 4.57SYY81 pKa = 11.28KK82 pKa = 10.03NTIKK86 pKa = 10.78PNSQQSMEE94 pKa = 4.61GIVRR98 pKa = 11.84LHH100 pKa = 6.54ILPVFGDD107 pKa = 3.69YY108 pKa = 10.92KK109 pKa = 10.98LNSLTTPIIQQQVNKK124 pKa = 9.3WADD127 pKa = 3.5RR128 pKa = 11.84ANRR131 pKa = 11.84GEE133 pKa = 3.89KK134 pKa = 9.89GAYY137 pKa = 9.71ANYY140 pKa = 10.84SFLNNINRR148 pKa = 11.84RR149 pKa = 11.84ILQYY153 pKa = 10.72GVTMQVIQHH162 pKa = 5.79NPARR166 pKa = 11.84DD167 pKa = 3.51VIIPRR172 pKa = 11.84KK173 pKa = 8.85QQAKK177 pKa = 6.27EE178 pKa = 3.48QKK180 pKa = 9.56IRR182 pKa = 11.84FFSNQEE188 pKa = 3.74LKK190 pKa = 10.95KK191 pKa = 10.46FLAYY195 pKa = 10.41LDD197 pKa = 4.4SLDD200 pKa = 4.03LNLYY204 pKa = 9.79EE205 pKa = 5.02NLFDD209 pKa = 3.74YY210 pKa = 10.98VLYY213 pKa = 8.9KK214 pKa = 10.39TLLASGCRR222 pKa = 11.84IGEE225 pKa = 4.09VLALEE230 pKa = 4.47WSDD233 pKa = 3.8IDD235 pKa = 5.14LKK237 pKa = 10.52TGTISISKK245 pKa = 8.18TLNRR249 pKa = 11.84YY250 pKa = 8.7QEE252 pKa = 4.35TNTPKK257 pKa = 10.61SKK259 pKa = 10.87AGLRR263 pKa = 11.84DD264 pKa = 3.45IEE266 pKa = 4.69IDD268 pKa = 3.36PATISLLKK276 pKa = 10.13QYY278 pKa = 10.83KK279 pKa = 9.51KK280 pKa = 10.33RR281 pKa = 11.84QQVEE285 pKa = 3.92CWKK288 pKa = 10.56LGRR291 pKa = 11.84SEE293 pKa = 5.56NIVFTPFTTKK303 pKa = 10.34YY304 pKa = 10.75AYY306 pKa = 10.29ACLLRR311 pKa = 11.84KK312 pKa = 9.66RR313 pKa = 11.84LQGHH317 pKa = 6.11FKK319 pKa = 10.67SAGVPDD325 pKa = 3.94ISFHH329 pKa = 5.86GFRR332 pKa = 11.84HH333 pKa = 4.4THH335 pKa = 4.65ATIMLYY341 pKa = 10.62AGIEE345 pKa = 4.18AKK347 pKa = 10.42DD348 pKa = 3.35LQYY351 pKa = 11.55RR352 pKa = 11.84LGHH355 pKa = 5.94SNITMTLNTYY365 pKa = 8.72VHH367 pKa = 6.14ATKK370 pKa = 10.47EE371 pKa = 4.25GAKK374 pKa = 9.7KK375 pKa = 10.18AVSIFEE381 pKa = 4.16TAISNLL387 pKa = 3.59

Molecular weight: 44.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1716

63

479

245.1

28.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.303 ± 0.715

0.699 ± 0.14

4.779 ± 0.938

8.858 ± 1.395

4.254 ± 0.833

5.245 ± 0.464

1.224 ± 0.289

6.119 ± 0.845

10.315 ± 0.673

9.732 ± 0.824

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.331 ± 0.596

6.177 ± 0.412

2.855 ± 0.369

3.671 ± 0.669

4.895 ± 0.844

5.594 ± 0.306

6.469 ± 0.676

5.828 ± 0.379

1.34 ± 0.384

4.312 ± 0.265
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski