Amino acid dipepetide frequency for Streptomyces sp. yr375

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.392AlaAla: 21.392 ± 0.167
1.12AlaCys: 1.12 ± 0.023
8.647AlaAsp: 8.647 ± 0.072
8.742AlaGlu: 8.742 ± 0.081
3.497AlaPhe: 3.497 ± 0.04
12.912AlaGly: 12.912 ± 0.088
3.006AlaHis: 3.006 ± 0.036
3.261AlaIle: 3.261 ± 0.039
2.861AlaLys: 2.861 ± 0.047
14.726AlaLeu: 14.726 ± 0.119
2.309AlaMet: 2.309 ± 0.027
2.007AlaAsn: 2.007 ± 0.03
7.211AlaPro: 7.211 ± 0.084
3.899AlaGln: 3.899 ± 0.046
10.257AlaArg: 10.257 ± 0.085
5.92AlaSer: 5.92 ± 0.051
7.387AlaThr: 7.387 ± 0.066
12.473AlaVal: 12.473 ± 0.095
1.86AlaTrp: 1.86 ± 0.028
2.835AlaTyr: 2.835 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
1.118CysAla: 1.118 ± 0.021
0.098CysCys: 0.098 ± 0.007
0.48CysAsp: 0.48 ± 0.013
0.402CysGlu: 0.402 ± 0.014
0.22CysPhe: 0.22 ± 0.009
0.918CysGly: 0.918 ± 0.019
0.189CysHis: 0.189 ± 0.009
0.152CysIle: 0.152 ± 0.008
0.126CysLys: 0.126 ± 0.007
0.735CysLeu: 0.735 ± 0.019
0.116CysMet: 0.116 ± 0.006
0.126CysAsn: 0.126 ± 0.008
0.451CysPro: 0.451 ± 0.014
0.175CysGln: 0.175 ± 0.008
0.599CysArg: 0.599 ± 0.016
0.441CysSer: 0.441 ± 0.014
0.521CysThr: 0.521 ± 0.013
0.689CysVal: 0.689 ± 0.015
0.125CysTrp: 0.125 ± 0.008
0.151CysTyr: 0.151 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.963AspAla: 7.963 ± 0.074
0.435AspCys: 0.435 ± 0.013
3.832AspAsp: 3.832 ± 0.046
3.802AspGlu: 3.802 ± 0.041
1.678AspPhe: 1.678 ± 0.026
6.551AspGly: 6.551 ± 0.067
1.483AspHis: 1.483 ± 0.024
1.751AspIle: 1.751 ± 0.03
1.145AspLys: 1.145 ± 0.026
6.263AspLeu: 6.263 ± 0.061
0.806AspMet: 0.806 ± 0.019
0.978AspAsn: 0.978 ± 0.022
4.601AspPro: 4.601 ± 0.043
1.574AspGln: 1.574 ± 0.022
4.859AspArg: 4.859 ± 0.047
2.634AspSer: 2.634 ± 0.035
3.525AspThr: 3.525 ± 0.038
4.923AspVal: 4.923 ± 0.047
1.084AspTrp: 1.084 ± 0.021
1.137AspTyr: 1.137 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
7.277GluAla: 7.277 ± 0.073
0.391GluCys: 0.391 ± 0.015
2.695GluAsp: 2.695 ± 0.032
3.273GluGlu: 3.273 ± 0.041
1.52GluPhe: 1.52 ± 0.026
4.139GluGly: 4.139 ± 0.043
1.487GluHis: 1.487 ± 0.024
2.251GluIle: 2.251 ± 0.029
1.316GluLys: 1.316 ± 0.028
6.875GluLeu: 6.875 ± 0.079
0.822GluMet: 0.822 ± 0.02
1.002GluAsn: 1.002 ± 0.022
3.335GluPro: 3.335 ± 0.042
2.221GluGln: 2.221 ± 0.031
5.253GluArg: 5.253 ± 0.06
2.604GluSer: 2.604 ± 0.034
3.062GluThr: 3.062 ± 0.034
4.322GluVal: 4.322 ± 0.044
0.767GluTrp: 0.767 ± 0.018
1.103GluTyr: 1.103 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
3.65PheAla: 3.65 ± 0.038
0.253PheCys: 0.253 ± 0.011
1.99PheAsp: 1.99 ± 0.033
1.409PheGlu: 1.409 ± 0.023
0.861PhePhe: 0.861 ± 0.019
3.002PheGly: 3.002 ± 0.036
0.634PheHis: 0.634 ± 0.018
0.649PheIle: 0.649 ± 0.019
0.507PheLys: 0.507 ± 0.014
2.554PheLeu: 2.554 ± 0.033
0.393PheMet: 0.393 ± 0.013
0.543PheAsn: 0.543 ± 0.018
1.394PhePro: 1.394 ± 0.025
0.695PheGln: 0.695 ± 0.016
1.832PheArg: 1.832 ± 0.026
1.415PheSer: 1.415 ± 0.023
2.036PheThr: 2.036 ± 0.031
2.28PheVal: 2.28 ± 0.032
0.418PheTrp: 0.418 ± 0.013
0.55PheTyr: 0.55 ± 0.014
0.0PheXaa: 0.0 ± 0.0
Gly
10.941GlyAla: 10.941 ± 0.088
0.813GlyCys: 0.813 ± 0.017
5.387GlyAsp: 5.387 ± 0.044
5.075GlyGlu: 5.075 ± 0.044
2.769GlyPhe: 2.769 ± 0.037
8.905GlyGly: 8.905 ± 0.097
2.378GlyHis: 2.378 ± 0.032
3.262GlyIle: 3.262 ± 0.043
2.36GlyLys: 2.36 ± 0.039
9.333GlyLeu: 9.333 ± 0.071
1.904GlyMet: 1.904 ± 0.029
1.798GlyAsn: 1.798 ± 0.035
5.088GlyPro: 5.088 ± 0.055
2.693GlyGln: 2.693 ± 0.037
7.615GlyArg: 7.615 ± 0.064
5.321GlySer: 5.321 ± 0.059
6.557GlyThr: 6.557 ± 0.064
7.876GlyVal: 7.876 ± 0.07
1.697GlyTrp: 1.697 ± 0.025
2.251GlyTyr: 2.251 ± 0.033
0.0GlyXaa: 0.0 ± 0.0
His
2.793HisAla: 2.793 ± 0.036
0.203HisCys: 0.203 ± 0.009
1.37HisAsp: 1.37 ± 0.024
1.231HisGlu: 1.231 ± 0.024
0.622HisPhe: 0.622 ± 0.017
2.415HisGly: 2.415 ± 0.035
0.728HisHis: 0.728 ± 0.019
0.657HisIle: 0.657 ± 0.015
0.342HisLys: 0.342 ± 0.012
2.471HisLeu: 2.471 ± 0.037
0.324HisMet: 0.324 ± 0.011
0.394HisAsn: 0.394 ± 0.012
1.891HisPro: 1.891 ± 0.031
0.654HisGln: 0.654 ± 0.019
2.095HisArg: 2.095 ± 0.03
1.058HisSer: 1.058 ± 0.018
1.461HisThr: 1.461 ± 0.023
1.742HisVal: 1.742 ± 0.028
0.377HisTrp: 0.377 ± 0.011
0.473HisTyr: 0.473 ± 0.013
0.0HisXaa: 0.0 ± 0.0
Ile
4.512IleAla: 4.512 ± 0.048
0.258IleCys: 0.258 ± 0.011
2.127IleAsp: 2.127 ± 0.031
1.835IleGlu: 1.835 ± 0.029
0.658IlePhe: 0.658 ± 0.018
3.355IleGly: 3.355 ± 0.039
0.594IleHis: 0.594 ± 0.016
0.795IleIle: 0.795 ± 0.019
0.707IleLys: 0.707 ± 0.016
2.336IleLeu: 2.336 ± 0.039
0.43IleMet: 0.43 ± 0.013
0.645IleAsn: 0.645 ± 0.016
1.719IlePro: 1.719 ± 0.027
0.705IleGln: 0.705 ± 0.016
2.154IleArg: 2.154 ± 0.035
1.583IleSer: 1.583 ± 0.025
2.109IleThr: 2.109 ± 0.03
2.624IleVal: 2.624 ± 0.039
0.338IleTrp: 0.338 ± 0.011
0.493IleTyr: 0.493 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
2.929LysAla: 2.929 ± 0.047
0.104LysCys: 0.104 ± 0.007
1.29LysAsp: 1.29 ± 0.026
1.104LysGlu: 1.104 ± 0.027
0.456LysPhe: 0.456 ± 0.012
1.806LysGly: 1.806 ± 0.032
0.407LysHis: 0.407 ± 0.014
0.845LysIle: 0.845 ± 0.019
0.839LysLys: 0.839 ± 0.026
2.029LysLeu: 2.029 ± 0.034
0.367LysMet: 0.367 ± 0.014
0.531LysAsn: 0.531 ± 0.017
1.291LysPro: 1.291 ± 0.029
0.704LysGln: 0.704 ± 0.017
1.396LysArg: 1.396 ± 0.03
1.221LysSer: 1.221 ± 0.023
1.359LysThr: 1.359 ± 0.024
1.852LysVal: 1.852 ± 0.032
0.285LysTrp: 0.285 ± 0.011
0.469LysTyr: 0.469 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
15.253LeuAla: 15.253 ± 0.119
0.852LeuCys: 0.852 ± 0.021
6.859LeuAsp: 6.859 ± 0.07
4.596LeuGlu: 4.596 ± 0.053
2.656LeuPhe: 2.656 ± 0.038
9.131LeuGly: 9.131 ± 0.073
2.339LeuHis: 2.339 ± 0.036
3.056LeuIle: 3.056 ± 0.042
2.074LeuLys: 2.074 ± 0.032
11.328LeuLeu: 11.328 ± 0.083
1.538LeuMet: 1.538 ± 0.024
1.685LeuAsn: 1.685 ± 0.03
6.669LeuPro: 6.669 ± 0.062
2.138LeuGln: 2.138 ± 0.03
8.771LeuArg: 8.771 ± 0.079
5.231LeuSer: 5.231 ± 0.047
7.362LeuThr: 7.362 ± 0.069
8.786LeuVal: 8.786 ± 0.06
1.358LeuTrp: 1.358 ± 0.024
1.873LeuTyr: 1.873 ± 0.029
0.0LeuXaa: 0.0 ± 0.0
Met
2.156MetAla: 2.156 ± 0.032
0.128MetCys: 0.128 ± 0.007
0.861MetAsp: 0.861 ± 0.019
0.689MetGlu: 0.689 ± 0.016
0.46MetPhe: 0.46 ± 0.015
1.241MetGly: 1.241 ± 0.025
0.321MetHis: 0.321 ± 0.011
0.631MetIle: 0.631 ± 0.018
0.388MetLys: 0.388 ± 0.012
1.538MetLeu: 1.538 ± 0.026
0.251MetMet: 0.251 ± 0.011
0.417MetAsn: 0.417 ± 0.013
1.048MetPro: 1.048 ± 0.025
0.412MetGln: 0.412 ± 0.013
1.345MetArg: 1.345 ± 0.025
1.294MetSer: 1.294 ± 0.023
1.514MetThr: 1.514 ± 0.027
1.25MetVal: 1.25 ± 0.027
0.21MetTrp: 0.21 ± 0.009
0.326MetTyr: 0.326 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.218AsnAla: 2.218 ± 0.032
0.174AsnCys: 0.174 ± 0.007
0.967AsnAsp: 0.967 ± 0.023
0.783AsnGlu: 0.783 ± 0.018
0.47AsnPhe: 0.47 ± 0.014
1.975AsnGly: 1.975 ± 0.034
0.399AsnHis: 0.399 ± 0.014
0.633AsnIle: 0.633 ± 0.015
0.413AsnLys: 0.413 ± 0.014
1.63AsnLeu: 1.63 ± 0.028
0.31AsnMet: 0.31 ± 0.011
0.443AsnAsn: 0.443 ± 0.016
1.357AsnPro: 1.357 ± 0.025
0.538AsnGln: 0.538 ± 0.016
1.25AsnArg: 1.25 ± 0.023
1.004AsnSer: 1.004 ± 0.021
1.21AsnThr: 1.21 ± 0.026
1.34AsnVal: 1.34 ± 0.024
0.285AsnTrp: 0.285 ± 0.011
0.421AsnTyr: 0.421 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.732ProAla: 8.732 ± 0.093
0.32ProCys: 0.32 ± 0.012
4.595ProAsp: 4.595 ± 0.052
4.305ProGlu: 4.305 ± 0.051
1.528ProPhe: 1.528 ± 0.024
6.483ProGly: 6.483 ± 0.064
1.459ProHis: 1.459 ± 0.026
1.259ProIle: 1.259 ± 0.025
1.209ProLys: 1.209 ± 0.023
5.508ProLeu: 5.508 ± 0.049
0.963ProMet: 0.963 ± 0.02
0.949ProAsn: 0.949 ± 0.02
3.679ProPro: 3.679 ± 0.058
1.861ProGln: 1.861 ± 0.04
3.869ProArg: 3.869 ± 0.05
3.211ProSer: 3.211 ± 0.04
3.633ProThr: 3.633 ± 0.041
5.642ProVal: 5.642 ± 0.056
0.899ProTrp: 0.899 ± 0.021
1.428ProTyr: 1.428 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.765GlnAla: 3.765 ± 0.047
0.167GlnCys: 0.167 ± 0.008
1.462GlnAsp: 1.462 ± 0.022
1.436GlnGlu: 1.436 ± 0.026
0.65GlnPhe: 0.65 ± 0.016
2.377GlnGly: 2.377 ± 0.041
0.671GlnHis: 0.671 ± 0.016
1.029GlnIle: 1.029 ± 0.021
0.613GlnLys: 0.613 ± 0.015
2.969GlnLeu: 2.969 ± 0.037
0.453GlnMet: 0.453 ± 0.015
0.534GlnAsn: 0.534 ± 0.018
1.835GlnPro: 1.835 ± 0.037
1.332GlnGln: 1.332 ± 0.035
2.411GlnArg: 2.411 ± 0.031
1.35GlnSer: 1.35 ± 0.026
1.432GlnThr: 1.432 ± 0.027
2.391GlnVal: 2.391 ± 0.029
0.459GlnTrp: 0.459 ± 0.012
0.637GlnTyr: 0.637 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
9.784ArgAla: 9.784 ± 0.088
0.569ArgCys: 0.569 ± 0.014
4.181ArgAsp: 4.181 ± 0.04
4.677ArgGlu: 4.677 ± 0.055
2.351ArgPhe: 2.351 ± 0.033
5.704ArgGly: 5.704 ± 0.053
2.14ArgHis: 2.14 ± 0.034
3.036ArgIle: 3.036 ± 0.037
1.59ArgLys: 1.59 ± 0.032
9.088ArgLeu: 9.088 ± 0.087
1.646ArgMet: 1.646 ± 0.027
1.281ArgAsn: 1.281 ± 0.022
4.973ArgPro: 4.973 ± 0.059
2.318ArgGln: 2.318 ± 0.033
7.48ArgArg: 7.48 ± 0.069
3.882ArgSer: 3.882 ± 0.036
5.462ArgThr: 5.462 ± 0.05
5.936ArgVal: 5.936 ± 0.052
1.338ArgTrp: 1.338 ± 0.025
1.775ArgTyr: 1.775 ± 0.025
0.0ArgXaa: 0.0 ± 0.0
Ser
6.879SerAla: 6.879 ± 0.06
0.395SerCys: 0.395 ± 0.013
2.782SerAsp: 2.782 ± 0.041
2.342SerGlu: 2.342 ± 0.033
1.488SerPhe: 1.488 ± 0.026
6.017SerGly: 6.017 ± 0.064
1.032SerHis: 1.032 ± 0.021
1.379SerIle: 1.379 ± 0.024
1.013SerLys: 1.013 ± 0.022
4.9SerLeu: 4.9 ± 0.041
1.05SerMet: 1.05 ± 0.021
0.897SerAsn: 0.897 ± 0.022
3.247SerPro: 3.247 ± 0.042
1.27SerGln: 1.27 ± 0.026
3.56SerArg: 3.56 ± 0.039
2.965SerSer: 2.965 ± 0.043
3.165SerThr: 3.165 ± 0.044
4.306SerVal: 4.306 ± 0.045
0.895SerTrp: 0.895 ± 0.018
1.281SerTyr: 1.281 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
9.511ThrAla: 9.511 ± 0.091
0.455ThrCys: 0.455 ± 0.014
3.869ThrAsp: 3.869 ± 0.048
3.302ThrGlu: 3.302 ± 0.042
1.621ThrPhe: 1.621 ± 0.026
7.07ThrGly: 7.07 ± 0.07
1.289ThrHis: 1.289 ± 0.026
1.665ThrIle: 1.665 ± 0.03
1.235ThrLys: 1.235 ± 0.027
5.949ThrLeu: 5.949 ± 0.052
0.912ThrMet: 0.912 ± 0.02
1.068ThrAsn: 1.068 ± 0.022
4.535ThrPro: 4.535 ± 0.045
1.389ThrGln: 1.389 ± 0.027
4.033ThrArg: 4.033 ± 0.043
3.374ThrSer: 3.374 ± 0.047
4.221ThrThr: 4.221 ± 0.061
6.266ThrVal: 6.266 ± 0.057
0.934ThrTrp: 0.934 ± 0.021
1.479ThrTyr: 1.479 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
10.924ValAla: 10.924 ± 0.075
0.766ValCys: 0.766 ± 0.019
5.155ValAsp: 5.155 ± 0.041
4.742ValGlu: 4.742 ± 0.048
2.435ValPhe: 2.435 ± 0.033
6.607ValGly: 6.607 ± 0.053
1.972ValHis: 1.972 ± 0.033
2.691ValIle: 2.691 ± 0.034
1.778ValLys: 1.778 ± 0.028
9.453ValLeu: 9.453 ± 0.084
1.364ValMet: 1.364 ± 0.024
1.699ValAsn: 1.699 ± 0.028
5.307ValPro: 5.307 ± 0.054
2.137ValGln: 2.137 ± 0.028
7.311ValArg: 7.311 ± 0.063
4.419ValSer: 4.419 ± 0.041
5.822ValThr: 5.822 ± 0.053
8.157ValVal: 8.157 ± 0.072
1.19ValTrp: 1.19 ± 0.022
1.584ValTyr: 1.584 ± 0.027
0.0ValXaa: 0.0 ± 0.0
Trp
1.682TrpAla: 1.682 ± 0.023
0.158TrpCys: 0.158 ± 0.008
0.837TrpAsp: 0.837 ± 0.019
0.747TrpGlu: 0.747 ± 0.017
0.503TrpPhe: 0.503 ± 0.014
1.096TrpGly: 1.096 ± 0.021
0.374TrpHis: 0.374 ± 0.011
0.506TrpIle: 0.506 ± 0.016
0.37TrpLys: 0.37 ± 0.013
1.774TrpLeu: 1.774 ± 0.031
0.275TrpMet: 0.275 ± 0.011
0.413TrpAsn: 0.413 ± 0.012
0.769TrpPro: 0.769 ± 0.019
0.65TrpGln: 0.65 ± 0.015
1.348TrpArg: 1.348 ± 0.024
0.999TrpSer: 0.999 ± 0.02
1.083TrpThr: 1.083 ± 0.022
0.96TrpVal: 0.96 ± 0.021
0.322TrpTrp: 0.322 ± 0.013
0.371TrpTyr: 0.371 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.841TyrAla: 2.841 ± 0.034
0.173TyrCys: 0.173 ± 0.007
1.637TyrAsp: 1.637 ± 0.034
1.223TyrGlu: 1.223 ± 0.026
0.622TyrPhe: 0.622 ± 0.015
2.318TyrGly: 2.318 ± 0.034
0.397TyrHis: 0.397 ± 0.013
0.468TyrIle: 0.468 ± 0.014
0.4TyrLys: 0.4 ± 0.015
2.096TyrLeu: 2.096 ± 0.03
0.246TyrMet: 0.246 ± 0.009
0.409TyrAsn: 0.409 ± 0.013
1.131TyrPro: 1.131 ± 0.023
0.611TyrGln: 0.611 ± 0.017
1.77TyrArg: 1.77 ± 0.028
0.943TyrSer: 0.943 ± 0.019
1.255TyrThr: 1.255 ± 0.029
1.7TyrVal: 1.7 ± 0.025
0.398TyrTrp: 0.398 ± 0.012
0.448TyrTyr: 0.448 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7671 proteins (2673518 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski