Staphylococcus phage SA13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus; Staphylococcus virus SA13

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4IFL1|R4IFL1_9CAUD Uncharacterized protein OS=Staphylococcus phage SA13 OX=1195086 GN=SA13_27 PE=4 SV=1
MM1 pKa = 6.89QQQAYY6 pKa = 9.38INATIDD12 pKa = 2.95IRR14 pKa = 11.84IPTEE18 pKa = 3.56VEE20 pKa = 3.88YY21 pKa = 11.22KK22 pKa = 10.82HH23 pKa = 6.75FGDD26 pKa = 4.47VDD28 pKa = 3.64NEE30 pKa = 3.66KK31 pKa = 10.93DD32 pKa = 3.32ALADD36 pKa = 3.51YY37 pKa = 10.65LYY39 pKa = 11.0NNPNEE44 pKa = 3.8ILEE47 pKa = 3.94YY48 pKa = 11.1DD49 pKa = 3.47NLKK52 pKa = 9.69IRR54 pKa = 11.84NVNVEE59 pKa = 3.93VEE61 pKa = 4.1

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4II69|R4II69_9CAUD Putative minor tail protein OS=Staphylococcus phage SA13 OX=1195086 GN=SA13_53 PE=4 SV=1
MM1 pKa = 6.63TSRR4 pKa = 11.84YY5 pKa = 8.73IGYY8 pKa = 8.71MSNDD12 pKa = 3.71EE13 pKa = 4.57LMSMLPAEE21 pKa = 4.27WNDD24 pKa = 3.65WIIGARR30 pKa = 11.84QALIDD35 pKa = 3.58QRR37 pKa = 11.84DD38 pKa = 3.38IALYY42 pKa = 8.51GAQYY46 pKa = 10.82NAVAQAGKK54 pKa = 9.21SLKK57 pKa = 10.52RR58 pKa = 11.84FVRR61 pKa = 11.84QNEE64 pKa = 3.97RR65 pKa = 11.84EE66 pKa = 4.01HH67 pKa = 7.72YY68 pKa = 9.82IIRR71 pKa = 11.84GQEE74 pKa = 3.65DD75 pKa = 4.37EE76 pKa = 4.41YY77 pKa = 11.34EE78 pKa = 4.13KK79 pKa = 10.59MKK81 pKa = 10.6QRR83 pKa = 11.84EE84 pKa = 4.01LAKK87 pKa = 10.34NKK89 pKa = 9.72HH90 pKa = 5.34KK91 pKa = 11.03RR92 pKa = 11.84EE93 pKa = 3.9IQKK96 pKa = 10.26QGTRR100 pKa = 11.84KK101 pKa = 9.39FLNSLKK107 pKa = 9.64TSHH110 pKa = 7.02KK111 pKa = 10.74GGG113 pKa = 3.51

Molecular weight: 13.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13140

52

989

211.9

24.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.898 ± 0.368

0.518 ± 0.082

6.4 ± 0.281

7.542 ± 0.461

4.391 ± 0.199

5.86 ± 0.333

1.651 ± 0.146

7.192 ± 0.226

9.277 ± 0.395

7.542 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.511 ± 0.153

6.438 ± 0.249

2.763 ± 0.201

4.011 ± 0.206

3.995 ± 0.2

5.997 ± 0.276

6.271 ± 0.25

6.073 ± 0.308

1.233 ± 0.164

4.437 ± 0.332
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski