Wuhan coneheads virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KMM9|A0A1L3KMM9_9VIRU Uncharacterized protein OS=Wuhan coneheads virus 2 OX=1923696 PE=4 SV=1
MM1 pKa = 7.37VSQSASSMPVMAEE14 pKa = 3.81PVLDD18 pKa = 4.02EE19 pKa = 5.23ALTTPTSLGNSTNLATTHH37 pKa = 6.91EE38 pKa = 4.34IANPCAMVVDD48 pKa = 4.28NPMASGRR55 pKa = 11.84PYY57 pKa = 11.28DD58 pKa = 3.52VFTWNRR64 pKa = 11.84KK65 pKa = 8.55HH66 pKa = 5.47YY67 pKa = 10.55QLIKK71 pKa = 10.02SGKK74 pKa = 7.47ITSTDD79 pKa = 2.86TDD81 pKa = 3.84GANLTFVTNNPSEE94 pKa = 4.0FSGLLGAEE102 pKa = 4.2ANLHH106 pKa = 5.61EE107 pKa = 5.11FCTPQIQYY115 pKa = 9.11VVRR118 pKa = 11.84IYY120 pKa = 9.92GTEE123 pKa = 4.22GIIGRR128 pKa = 11.84CLVAAGDD135 pKa = 3.85SSLFISNKK143 pKa = 9.56FEE145 pKa = 4.88AGTGLSDD152 pKa = 4.91ALSAPYY158 pKa = 9.15WRR160 pKa = 11.84YY161 pKa = 9.91ISFNGVSSIEE171 pKa = 3.92FTLNDD176 pKa = 3.07ARR178 pKa = 11.84KK179 pKa = 9.16DD180 pKa = 3.39GFYY183 pKa = 11.0RR184 pKa = 11.84SMTDD188 pKa = 4.94ASSTWQYY195 pKa = 11.62LGIFLVQAPRR205 pKa = 11.84SKK207 pKa = 10.76YY208 pKa = 8.66ATSTATTTDD217 pKa = 3.4VVSLDD222 pKa = 3.19WEE224 pKa = 3.9IFARR228 pKa = 11.84YY229 pKa = 8.73GPRR232 pKa = 11.84HH233 pKa = 5.54MFFGPRR239 pKa = 11.84LVKK242 pKa = 9.32PTTLTLNFTTTLQSFMPAAFDD263 pKa = 3.47FCYY266 pKa = 10.57GSAYY270 pKa = 9.83IPDD273 pKa = 4.57DD274 pKa = 3.71NEE276 pKa = 3.92KK277 pKa = 10.62HH278 pKa = 4.25VTFPTFLQADD288 pKa = 4.4GSLKK292 pKa = 10.38FDD294 pKa = 5.09LSAQSYY300 pKa = 8.52IGSRR304 pKa = 11.84KK305 pKa = 9.48VRR307 pKa = 11.84DD308 pKa = 4.09CYY310 pKa = 10.99TNTIVSVGQVAEE322 pKa = 4.13EE323 pKa = 3.95LDD325 pKa = 4.13IISGASGFNWGVSDD339 pKa = 5.2EE340 pKa = 4.15VMQRR344 pKa = 11.84CMNFGLFQFWGEE356 pKa = 4.44GEE358 pKa = 4.39VSQSLLDD365 pKa = 3.59KK366 pKa = 10.89LDD368 pKa = 4.29NIKK371 pKa = 10.8VLLDD375 pKa = 3.35DD376 pKa = 4.99TFPLMLDD383 pKa = 3.51TPVTFISDD391 pKa = 3.32QEE393 pKa = 3.96YY394 pKa = 10.06DD395 pKa = 3.66YY396 pKa = 11.56SGSEE400 pKa = 3.87LDD402 pKa = 3.68PYY404 pKa = 11.62EE405 pKa = 4.49MIKK408 pKa = 10.64KK409 pKa = 10.0ADD411 pKa = 3.77NKK413 pKa = 10.28QVIQIRR419 pKa = 11.84GCITSGKK426 pKa = 8.17GRR428 pKa = 11.84IWITEE433 pKa = 3.9PSQSHH438 pKa = 5.13VMSSFGDD445 pKa = 3.37VGILYY450 pKa = 9.54FYY452 pKa = 10.96SNKK455 pKa = 10.39NGTKK459 pKa = 9.75RR460 pKa = 11.84PEE462 pKa = 3.7WTSALWGDD470 pKa = 4.12DD471 pKa = 3.23VHH473 pKa = 8.39DD474 pKa = 4.79RR475 pKa = 11.84DD476 pKa = 4.15AGAMAMAYY484 pKa = 10.58YY485 pKa = 10.44EE486 pKa = 4.27DD487 pKa = 4.41AEE489 pKa = 4.69NNVEE493 pKa = 4.07APFIEE498 pKa = 4.64TVSPIISSFKK508 pKa = 10.83LEE510 pKa = 3.97QEE512 pKa = 4.13LDD514 pKa = 3.82DD515 pKa = 5.96KK516 pKa = 11.32VVEE519 pKa = 3.92WTYY522 pKa = 11.39CVNNYY527 pKa = 10.79DD528 pKa = 3.47KK529 pKa = 11.51VKK531 pKa = 10.65FLGPRR536 pKa = 11.84LAEE539 pKa = 4.11LQFKK543 pKa = 10.71RR544 pKa = 11.84PGSSVINKK552 pKa = 8.2SVRR555 pKa = 11.84TTPGFEE561 pKa = 3.98AAGSSDD567 pKa = 3.28FNNYY571 pKa = 9.75LEE573 pKa = 4.81AIRR576 pKa = 11.84RR577 pKa = 11.84QFKK580 pKa = 10.51CDD582 pKa = 4.15LIGYY586 pKa = 6.52TLTINGRR593 pKa = 11.84YY594 pKa = 7.93YY595 pKa = 11.1CDD597 pKa = 2.89VAFYY601 pKa = 10.32KK602 pKa = 10.54GHH604 pKa = 6.36NVVCTDD610 pKa = 3.33TNFQILRR617 pKa = 11.84NMTQEE622 pKa = 3.85IEE624 pKa = 4.3VTNIRR629 pKa = 11.84PIRR632 pKa = 11.84TLGEE636 pKa = 3.83LRR638 pKa = 11.84TASVLPTYY646 pKa = 7.92WTVEE650 pKa = 3.84ATEE653 pKa = 4.47SFFPEE658 pKa = 3.91HH659 pKa = 6.36LKK661 pKa = 10.79EE662 pKa = 4.44FSSQSAGWMIGGGILSGIGSAIGTWQQQQFQEE694 pKa = 4.21ATQKK698 pKa = 10.98RR699 pKa = 11.84NLDD702 pKa = 3.57YY703 pKa = 10.62LTTRR707 pKa = 11.84DD708 pKa = 3.77EE709 pKa = 4.53LNRR712 pKa = 11.84LQQQVLQAQAHH723 pKa = 5.5QYY725 pKa = 7.48QQQGFWQRR733 pKa = 11.84AGVGATAAGGLATNSAEE750 pKa = 4.24TSSTEE755 pKa = 3.81TSKK758 pKa = 11.46DD759 pKa = 3.15STTPNVTPEE768 pKa = 4.2GLSVLQGNQDD778 pKa = 3.35KK779 pKa = 11.38LEE781 pKa = 3.98ASINYY786 pKa = 8.68KK787 pKa = 10.39PLVQVSDD794 pKa = 4.16FNLDD798 pKa = 3.33EE799 pKa = 5.1PYY801 pKa = 9.42VTSSKK806 pKa = 10.41VAQKK810 pKa = 10.29PYY812 pKa = 9.75NGPMHH817 pKa = 7.24TGPPIPPTNVGFKK830 pKa = 10.22LYY832 pKa = 10.7HH833 pKa = 6.76KK834 pKa = 9.98ISNPNTIEE842 pKa = 4.14NEE844 pKa = 3.76ATQAA848 pKa = 3.37

Molecular weight: 94.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KM50|A0A1L3KM50_9VIRU Uncharacterized protein OS=Wuhan coneheads virus 2 OX=1923696 PE=4 SV=1
MM1 pKa = 7.61LKK3 pKa = 9.34MEE5 pKa = 4.46TLKK8 pKa = 10.8LSKK11 pKa = 10.69VFTGRR16 pKa = 11.84IFCAEE21 pKa = 4.14DD22 pKa = 3.84LIGVLMEE29 pKa = 4.22RR30 pKa = 11.84KK31 pKa = 9.31FLGEE35 pKa = 3.95LYY37 pKa = 10.59ARR39 pKa = 11.84AMKK42 pKa = 10.1FDD44 pKa = 4.29PYY46 pKa = 11.3VGVGCNPMTGFDD58 pKa = 3.32AYY60 pKa = 10.38FRR62 pKa = 11.84RR63 pKa = 11.84ASPFPHH69 pKa = 6.67RR70 pKa = 11.84FVIDD74 pKa = 3.9FKK76 pKa = 11.24RR77 pKa = 11.84WDD79 pKa = 3.36KK80 pKa = 10.53TLAKK84 pKa = 10.15SLVKK88 pKa = 10.19ILRR91 pKa = 11.84TSLGEE96 pKa = 3.86VNPNLKK102 pKa = 9.89QCFNSVFNSVYY113 pKa = 8.54EE114 pKa = 4.34TYY116 pKa = 10.13HH117 pKa = 6.94ISGTTMYY124 pKa = 10.03YY125 pKa = 10.3RR126 pKa = 11.84SNGQPSGSAGTAVTNSLYY144 pKa = 11.05NLILTYY150 pKa = 10.13TVFSVLYY157 pKa = 10.09KK158 pKa = 10.23EE159 pKa = 5.07QFYY162 pKa = 10.65CYY164 pKa = 7.71PTWEE168 pKa = 4.08EE169 pKa = 3.66FKK171 pKa = 10.91RR172 pKa = 11.84AVQCFFYY179 pKa = 10.99GDD181 pKa = 3.73DD182 pKa = 3.53QFICTVYY189 pKa = 9.65PWFNRR194 pKa = 11.84VTFAAKK200 pKa = 10.11VKK202 pKa = 10.43EE203 pKa = 4.31LFNMNADD210 pKa = 3.84AMDD213 pKa = 3.86KK214 pKa = 10.7AGKK217 pKa = 9.21EE218 pKa = 3.81LSPFDD223 pKa = 3.65EE224 pKa = 4.76WEE226 pKa = 4.09DD227 pKa = 4.09CNWISRR233 pKa = 11.84YY234 pKa = 7.19WRR236 pKa = 11.84KK237 pKa = 9.81LDD239 pKa = 3.51EE240 pKa = 4.29FDD242 pKa = 4.66FRR244 pKa = 11.84VGALKK249 pKa = 10.65KK250 pKa = 10.06MSIGAQFHH258 pKa = 4.94WTSNSEE264 pKa = 3.86ISQIAHH270 pKa = 6.67NIQWALEE277 pKa = 3.95EE278 pKa = 3.87ASIWDD283 pKa = 3.35EE284 pKa = 3.91EE285 pKa = 4.85YY286 pKa = 11.07YY287 pKa = 11.01EE288 pKa = 4.35KK289 pKa = 10.91VKK291 pKa = 10.48QAARR295 pKa = 11.84IILQHH300 pKa = 5.83FPQYY304 pKa = 10.19RR305 pKa = 11.84GEE307 pKa = 4.05FYY309 pKa = 10.49IPSRR313 pKa = 11.84EE314 pKa = 4.0SLQLRR319 pKa = 11.84IYY321 pKa = 11.06NEE323 pKa = 3.63ALTPKK328 pKa = 10.77NLMAQSNEE336 pKa = 3.43AEE338 pKa = 3.85RR339 pKa = 11.84RR340 pKa = 11.84LNSMNIEE347 pKa = 4.09KK348 pKa = 10.73LLVFQPSGKK357 pKa = 8.02TNDD360 pKa = 3.63MASMILNRR368 pKa = 11.84FVQVNDD374 pKa = 3.37VKK376 pKa = 11.29LKK378 pKa = 9.57TDD380 pKa = 3.46YY381 pKa = 10.58TRR383 pKa = 11.84EE384 pKa = 4.45GPQHH388 pKa = 6.94LSMWTCIMTLDD399 pKa = 3.86NYY401 pKa = 10.02RR402 pKa = 11.84VCAEE406 pKa = 3.91AHH408 pKa = 5.57SKK410 pKa = 10.54KK411 pKa = 9.75IARR414 pKa = 11.84EE415 pKa = 3.66MASQKK420 pKa = 9.8MCDD423 pKa = 3.48KK424 pKa = 10.97LGLSLGSKK432 pKa = 10.09FRR434 pKa = 11.84QEE436 pKa = 4.16CDD438 pKa = 3.0AKK440 pKa = 10.72IATLWKK446 pKa = 9.59EE447 pKa = 3.86LARR450 pKa = 11.84LSLVRR455 pKa = 11.84DD456 pKa = 3.4QCSSKK461 pKa = 10.8KK462 pKa = 10.17FGRR465 pKa = 11.84PSYY468 pKa = 10.15EE469 pKa = 4.1DD470 pKa = 3.57SSDD473 pKa = 3.57SSSEE477 pKa = 4.16SEE479 pKa = 4.62DD480 pKa = 3.51FF481 pKa = 4.44

Molecular weight: 56.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3522

481

2193

1174.0

133.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.735 ± 0.576

1.76 ± 0.196

4.656 ± 0.422

6.871 ± 0.464

5.139 ± 0.379

5.764 ± 0.485

1.988 ± 0.311

6.133 ± 0.644

6.218 ± 0.679

7.865 ± 0.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.266

5.196 ± 0.104

4.969 ± 0.574

4.514 ± 0.222

4.373 ± 0.362

7.325 ± 0.653

6.502 ± 0.658

6.871 ± 0.704

1.533 ± 0.237

4.117 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski