Helicobacter sp. 11S03491-1
Average proteome isoelectric point is 7.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1649 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A268T8J3|A0A268T8J3_9HELI Uncharacterized protein OS=Helicobacter sp. 11S03491-1 OX=1476196 GN=BKH45_08440 PE=4 SV=1
MM1 pKa = 7.68 NIQEE5 pKa = 4.65 LDD7 pKa = 3.46 LGFSLKK13 pKa = 10.79 DD14 pKa = 3.52 VVRR17 pKa = 11.84 SDD19 pKa = 3.62 YY20 pKa = 11.26 FNGEE24 pKa = 3.89 VYY26 pKa = 10.67 LVVLEE31 pKa = 5.5 DD32 pKa = 3.64 EE33 pKa = 5.01 TGDD36 pKa = 4.37 CLDD39 pKa = 4.52 SLSGIYY45 pKa = 10.16 GLEE48 pKa = 4.1 YY49 pKa = 10.97 AEE51 pKa = 5.12 SEE53 pKa = 4.32 AKK55 pKa = 10.37 QMLLGAA61 pKa = 4.99
Molecular weight: 6.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.745
IPC_protein 3.63
Toseland 3.452
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.567
Rodwell 3.478
Grimsley 3.376
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 3.897
Thurlkill 3.516
EMBOSS 3.567
Sillero 3.745
Patrickios 0.604
IPC_peptide 3.579
IPC2_peptide 3.719
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|A0A268TC32|A0A268TC32_9HELI Uncharacterized protein OS=Helicobacter sp. 11S03491-1 OX=1476196 GN=BKH45_04815 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.34 RR14 pKa = 11.84 THH16 pKa = 6.03 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MKK23 pKa = 8.96 TKK25 pKa = 10.43 NGRR28 pKa = 11.84 KK29 pKa = 9.3 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.75 GRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 LAVV44 pKa = 3.15
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1649
0
1649
537068
37
4402
325.7
36.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.154 ± 0.061
1.127 ± 0.029
5.22 ± 0.042
5.952 ± 0.062
5.232 ± 0.059
6.481 ± 0.065
1.97 ± 0.026
9.643 ± 0.07
8.537 ± 0.066
9.876 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.36 ± 0.029
5.681 ± 0.068
3.341 ± 0.034
3.903 ± 0.042
3.231 ± 0.039
6.754 ± 0.057
4.593 ± 0.046
5.136 ± 0.054
0.776 ± 0.02
4.033 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here