Human associated huchismacovirus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Huchismacovirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5GG74|A0A0B5GG74_9VIRU Rep protein OS=Human associated huchismacovirus 3 OX=2169936 GN=rep PE=4 SV=1
MM1 pKa = 6.3MQKK4 pKa = 9.97FRR6 pKa = 11.84WFVDD10 pKa = 4.0LSTTPEE16 pKa = 3.89RR17 pKa = 11.84VSVLKK22 pKa = 9.05ITCGGTRR29 pKa = 11.84CLRR32 pKa = 11.84RR33 pKa = 11.84LLPFFLVLISIFKK46 pKa = 10.3LGKK49 pKa = 8.25TSVKK53 pKa = 9.9FVPSSTLPVDD63 pKa = 3.64PTGLSYY69 pKa = 10.68EE70 pKa = 4.23AGEE73 pKa = 4.26NTVDD77 pKa = 3.7PRR79 pKa = 11.84DD80 pKa = 3.66MFNPGLVRR88 pKa = 11.84ITNGEE93 pKa = 3.99DD94 pKa = 3.13LADD97 pKa = 3.4VVLNNISTAEE107 pKa = 4.02DD108 pKa = 3.44QNNVYY113 pKa = 10.21YY114 pKa = 11.53AMMLDD119 pKa = 3.52SRR121 pKa = 11.84WYY123 pKa = 10.46KK124 pKa = 10.42FRR126 pKa = 11.84LQDD129 pKa = 3.24GFRR132 pKa = 11.84RR133 pKa = 11.84DD134 pKa = 3.66AVPLYY139 pKa = 9.61WRR141 pKa = 11.84IGQLHH146 pKa = 6.19QDD148 pKa = 3.71NFPGSVNNYY157 pKa = 7.94LTVKK161 pKa = 10.51SDD163 pKa = 3.4VTKK166 pKa = 10.88SPTVSHH172 pKa = 6.99EE173 pKa = 3.56IGYY176 pKa = 9.2YY177 pKa = 10.06AYY179 pKa = 10.27SSPGSASPDD188 pKa = 2.97RR189 pKa = 11.84KK190 pKa = 10.31NQILLGSSPHH200 pKa = 5.59GTFQFGHH207 pKa = 6.67HH208 pKa = 7.19DD209 pKa = 3.93KK210 pKa = 11.0IGWLPTDD217 pKa = 3.76MFTNNYY223 pKa = 9.04VNTSGTPTMSPRR235 pKa = 11.84TPPEE239 pKa = 3.59VDD241 pKa = 3.4LLTVVLPAAYY251 pKa = 7.44KK252 pKa = 8.85TRR254 pKa = 11.84FYY256 pKa = 10.76YY257 pKa = 10.28RR258 pKa = 11.84LYY260 pKa = 9.16ITEE263 pKa = 3.9EE264 pKa = 4.72VYY266 pKa = 10.79FKK268 pKa = 11.13EE269 pKa = 4.21PVSIFAGQLSDD280 pKa = 3.41GQYY283 pKa = 11.18VSTTMDD289 pKa = 3.11RR290 pKa = 11.84FTYY293 pKa = 7.8PTLCGKK299 pKa = 10.16FPGNLNGVPSGTFLPVEE316 pKa = 4.61LDD318 pKa = 3.27NSGGDD323 pKa = 3.59TPP325 pKa = 5.24

Molecular weight: 36.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5GG74|A0A0B5GG74_9VIRU Rep protein OS=Human associated huchismacovirus 3 OX=2169936 GN=rep PE=4 SV=1
MM1 pKa = 8.0PSPKK5 pKa = 9.58WYY7 pKa = 9.93DD8 pKa = 2.92ITASKK13 pKa = 10.69EE14 pKa = 3.7KK15 pKa = 10.42LPRR18 pKa = 11.84SILEE22 pKa = 3.43KK23 pKa = 9.95WLNEE27 pKa = 3.72RR28 pKa = 11.84CEE30 pKa = 3.58RR31 pKa = 11.84WAYY34 pKa = 9.79GNEE37 pKa = 4.06VGEE40 pKa = 4.6GGYY43 pKa = 8.44EE44 pKa = 3.75HH45 pKa = 6.4YY46 pKa = 10.46QIRR49 pKa = 11.84VVLKK53 pKa = 9.87EE54 pKa = 3.86PTDD57 pKa = 3.66EE58 pKa = 4.3YY59 pKa = 11.41EE60 pKa = 4.06MRR62 pKa = 11.84KK63 pKa = 7.73MWAAFGHH70 pKa = 5.77VSPTHH75 pKa = 5.49VRR77 pKa = 11.84NFDD80 pKa = 3.63YY81 pKa = 10.87VLKK84 pKa = 10.68EE85 pKa = 3.62GDD87 pKa = 4.61YY88 pKa = 8.56VCSWIKK94 pKa = 10.53IPEE97 pKa = 4.04NMQAATFRR105 pKa = 11.84PWQRR109 pKa = 11.84ALLDD113 pKa = 3.89LEE115 pKa = 4.19QDD117 pKa = 3.5DD118 pKa = 4.25RR119 pKa = 11.84QIDD122 pKa = 4.13VIIDD126 pKa = 3.58LAGNTGKK133 pKa = 10.0SWLTKK138 pKa = 9.6YY139 pKa = 10.33CALKK143 pKa = 9.74MRR145 pKa = 11.84AINVPSGLEE154 pKa = 3.86GKK156 pKa = 10.17DD157 pKa = 3.27IMRR160 pKa = 11.84MCLKK164 pKa = 10.52RR165 pKa = 11.84GAQSGLYY172 pKa = 9.5IFDD175 pKa = 3.76MPRR178 pKa = 11.84AITKK182 pKa = 9.11QSKK185 pKa = 9.26SVWSAIEE192 pKa = 4.23SIKK195 pKa = 10.93NGYY198 pKa = 8.87LWDD201 pKa = 4.71DD202 pKa = 3.86RR203 pKa = 11.84YY204 pKa = 8.77TWEE207 pKa = 4.92EE208 pKa = 3.62KK209 pKa = 10.09WIDD212 pKa = 3.66PPRR215 pKa = 11.84IWVFTNEE222 pKa = 3.93YY223 pKa = 9.29PKK225 pKa = 10.9YY226 pKa = 10.45DD227 pKa = 3.78LVSEE231 pKa = 4.11DD232 pKa = 4.79RR233 pKa = 11.84LRR235 pKa = 11.84FWAPTPTGLVSLAHH249 pKa = 6.38

Molecular weight: 29.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

574

249

325

287.0

32.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.355 ± 1.107

1.22 ± 0.257

6.272 ± 0.102

5.226 ± 1.595

4.355 ± 1.291

6.794 ± 0.777

1.568 ± 0.026

4.704 ± 1.142

5.401 ± 1.213

8.537 ± 0.601

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.282

4.355 ± 0.758

6.794 ± 0.777

2.787 ± 0.016

5.923 ± 0.6

6.969 ± 0.893

6.969 ± 1.692

6.794 ± 1.044

2.962 ± 1.499

5.226 ± 0.004
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski