Absicoccus porci
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2184 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N0I2W6|A0A3N0I2W6_9FIRM SGNH_hydro domain-containing protein OS=Absicoccus porci OX=2486576 GN=EDX97_02030 PE=4 SV=1
MM1 pKa = 7.55 EE2 pKa = 4.98 VNCDD6 pKa = 3.45 MDD8 pKa = 4.4 LKK10 pKa = 10.26 PYY12 pKa = 10.9 SKK14 pKa = 8.12 TTIVNDD20 pKa = 4.0 DD21 pKa = 3.27 SDD23 pKa = 3.97 MTLLINKK30 pKa = 8.58 FYY32 pKa = 10.94 ALPQGYY38 pKa = 9.32 EE39 pKa = 3.78 PDD41 pKa = 3.82 DD42 pKa = 3.73 LVTIKK47 pKa = 10.63 DD48 pKa = 3.79 YY49 pKa = 11.68 ACVQGEE55 pKa = 4.64 DD56 pKa = 4.33 YY57 pKa = 11.19 SCQDD61 pKa = 3.01 VSEE64 pKa = 4.2 LTLRR68 pKa = 11.84 KK69 pKa = 9.68
Molecular weight: 7.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.961
IPC_protein 3.897
Toseland 3.681
ProMoST 4.088
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.732
Grimsley 3.592
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.279
Thurlkill 3.757
EMBOSS 3.872
Sillero 4.024
Patrickios 0.846
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.904
Protein with the highest isoelectric point:
>tr|A0A3N0I0I5|A0A3N0I0I5_9FIRM ATP-dependent zinc metalloprotease FtsH OS=Absicoccus porci OX=2486576 GN=hflB PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSKK9 pKa = 10.05 KK10 pKa = 9.55 KK11 pKa = 9.75 HH12 pKa = 4.68 QRR14 pKa = 11.84 THH16 pKa = 5.24 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.22 GGRR28 pKa = 11.84 KK29 pKa = 9.07 VLSSRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.71 GRR39 pKa = 11.84 KK40 pKa = 8.74 VLSAA44 pKa = 4.05
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.365
IPC2_protein 11.008
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.34
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.076
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 8.991
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2184
0
2184
652579
32
2369
298.8
33.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.011 ± 0.059
1.487 ± 0.022
6.206 ± 0.043
6.353 ± 0.052
4.093 ± 0.044
6.341 ± 0.056
2.464 ± 0.027
7.817 ± 0.049
7.106 ± 0.051
8.63 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.166 ± 0.028
4.511 ± 0.036
3.404 ± 0.027
4.244 ± 0.039
3.877 ± 0.037
5.779 ± 0.044
5.666 ± 0.06
6.68 ± 0.043
0.969 ± 0.017
4.194 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here