Mycoplasma gallinaceum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 768 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A449A2N7|A0A449A2N7_9MOLU ABC transporter ATP-binding protein OS=Mycoplasma gallinaceum OX=29556 GN=lagD PE=3 SV=1
MM1 pKa = 7.51LNEE4 pKa = 4.86IYY6 pKa = 10.46NQNSLIEE13 pKa = 4.09QSPLIQNYY21 pKa = 9.37VGEE24 pKa = 4.53FLMQEE29 pKa = 4.32SNQEE33 pKa = 4.11SEE35 pKa = 4.15FLKK38 pKa = 9.76ITFFCPNSYY47 pKa = 10.1KK48 pKa = 10.64CSLL51 pKa = 3.53

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A449A1Y6|A0A449A1Y6_9MOLU Transposase domain-containing protein OS=Mycoplasma gallinaceum OX=29556 GN=NCTC10183_00010 PE=4 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84KK4 pKa = 9.46ALIEE8 pKa = 3.93KK9 pKa = 10.2AKK11 pKa = 10.03RR12 pKa = 11.84HH13 pKa = 5.81PKK15 pKa = 10.03FSTRR19 pKa = 11.84AYY21 pKa = 8.48TRR23 pKa = 11.84CEE25 pKa = 3.8LCGRR29 pKa = 11.84PHH31 pKa = 7.07AVLRR35 pKa = 11.84KK36 pKa = 9.32YY37 pKa = 9.94KK38 pKa = 10.01VCRR41 pKa = 11.84ICFRR45 pKa = 11.84NLAHH49 pKa = 6.91EE50 pKa = 4.49GKK52 pKa = 10.2IPGIKK57 pKa = 9.11KK58 pKa = 10.56ASWW61 pKa = 2.94

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

768

0

768

306765

36

4918

399.4

45.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.809 ± 0.117

0.432 ± 0.024

5.685 ± 0.102

7.066 ± 0.092

5.35 ± 0.12

4.642 ± 0.102

1.34 ± 0.041

8.767 ± 0.109

9.865 ± 0.085

9.444 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.674 ± 0.054

7.995 ± 0.19

2.608 ± 0.052

3.555 ± 0.074

2.923 ± 0.054

6.849 ± 0.069

5.191 ± 0.105

5.754 ± 0.077

0.91 ± 0.023

4.141 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski