Shigella phage Buco

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Bucovirus; Shigella virus Buco

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482JMG8|A0A482JMG8_9CAUD Putative tail protein OS=Shigella phage Buco OX=2530183 GN=HRP29_gp44 PE=4 SV=1
MM1 pKa = 7.65AYY3 pKa = 9.5SWSEE7 pKa = 3.64QVVPSGTTLISVDD20 pKa = 3.48IEE22 pKa = 4.32YY23 pKa = 10.75LDD25 pKa = 3.27KK26 pKa = 11.22SYY28 pKa = 10.84IYY30 pKa = 10.6LYY32 pKa = 10.15INNVLISNSDD42 pKa = 3.57YY43 pKa = 11.36SWNSDD48 pKa = 3.33TLIQLNTPMASAGTVLLVRR67 pKa = 11.84RR68 pKa = 11.84TDD70 pKa = 3.27KK71 pKa = 10.82EE72 pKa = 3.75YY73 pKa = 10.84LYY75 pKa = 10.82IMFAEE80 pKa = 5.24GAAFIRR86 pKa = 11.84EE87 pKa = 4.04NLDD90 pKa = 3.22VQNTQFLHH98 pKa = 6.45LAQEE102 pKa = 4.35LVEE105 pKa = 4.43GRR107 pKa = 11.84SIDD110 pKa = 3.6GFYY113 pKa = 11.32GDD115 pKa = 5.43LSMNGYY121 pKa = 10.3RR122 pKa = 11.84ITHH125 pKa = 6.94LADD128 pKa = 3.65GVDD131 pKa = 3.84PKK133 pKa = 11.29DD134 pKa = 3.7AVNKK138 pKa = 9.69GQLDD142 pKa = 3.9SVSNRR147 pKa = 11.84VSSIEE152 pKa = 3.79NSFLGLTTVSYY163 pKa = 9.48PWYY166 pKa = 8.45TVVSADD172 pKa = 3.38TDD174 pKa = 3.72TFEE177 pKa = 5.46PPFKK181 pKa = 9.57FTKK184 pKa = 9.81AALYY188 pKa = 9.99IDD190 pKa = 5.02GLCQVPDD197 pKa = 3.42YY198 pKa = 11.21SYY200 pKa = 11.77VVVDD204 pKa = 4.1NKK206 pKa = 10.96LLLAEE211 pKa = 4.44SVPTGTVVFARR222 pKa = 11.84LGEE225 pKa = 4.38DD226 pKa = 2.86TDD228 pKa = 4.09AATEE232 pKa = 4.08AATTTALAAVQADD245 pKa = 3.96LQNQINALRR254 pKa = 11.84ALLQGGG260 pKa = 3.68

Molecular weight: 28.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482JKS3|A0A482JKS3_9CAUD DNA polymerase OS=Shigella phage Buco OX=2530183 GN=HRP29_gp19 PE=4 SV=1
MM1 pKa = 7.76ALTINPATVLEE12 pKa = 4.13RR13 pKa = 11.84QQRR16 pKa = 11.84SSIQGLARR24 pKa = 11.84EE25 pKa = 4.38YY26 pKa = 10.44SAKK29 pKa = 10.41AALRR33 pKa = 11.84TMYY36 pKa = 9.62EE37 pKa = 3.44QRR39 pKa = 11.84AAKK42 pKa = 10.3LGMTLAGYY50 pKa = 7.3CQRR53 pKa = 11.84FNIRR57 pKa = 11.84GVV59 pKa = 3.13

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

13156

34

1279

248.2

27.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.74 ± 0.579

1.026 ± 0.186

6.195 ± 0.196

5.822 ± 0.291

2.926 ± 0.147

7.631 ± 0.309

1.946 ± 0.203

4.576 ± 0.271

4.842 ± 0.281

8.916 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.919 ± 0.221

4.272 ± 0.287

3.907 ± 0.192

4.811 ± 0.267

5.701 ± 0.296

6.142 ± 0.283

5.549 ± 0.221

6.849 ± 0.22

1.414 ± 0.113

3.816 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski