Streptococcus phage Javan284

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AX25|A0A4D6AX25_9CAUD Integrase OS=Streptococcus phage Javan284 OX=2548093 GN=Javan284_0051 PE=4 SV=1
MM1 pKa = 7.31SRR3 pKa = 11.84IEE5 pKa = 3.77TSIAEE10 pKa = 4.26MYY12 pKa = 9.82NLQRR16 pKa = 11.84IPVHH20 pKa = 7.08YY21 pKa = 10.95DD22 pKa = 2.48MGDD25 pKa = 3.2RR26 pKa = 11.84YY27 pKa = 11.26GNDD30 pKa = 3.3ADD32 pKa = 4.42RR33 pKa = 11.84DD34 pKa = 3.87GLIEE38 pKa = 4.2YY39 pKa = 9.11DD40 pKa = 3.73CSSAVSKK47 pKa = 11.09ALGISLSNNTEE58 pKa = 4.09SLKK61 pKa = 11.27SNLPKK66 pKa = 9.93IGYY69 pKa = 8.46NCFYY73 pKa = 10.77DD74 pKa = 4.76GVDD77 pKa = 3.42GTFDD81 pKa = 3.32AVRR84 pKa = 11.84GDD86 pKa = 3.67VVIWGPRR93 pKa = 11.84DD94 pKa = 3.41GLSSLGAFGHH104 pKa = 5.5VLIFVDD110 pKa = 4.75GSNVIHH116 pKa = 6.9CNYY119 pKa = 9.97GSDD122 pKa = 3.26GVTVNDD128 pKa = 3.95YY129 pKa = 11.31NYY131 pKa = 9.83LWRR134 pKa = 11.84INGCPRR140 pKa = 11.84EE141 pKa = 4.18TVFRR145 pKa = 11.84EE146 pKa = 3.52NADD149 pKa = 4.6AINQEE154 pKa = 4.38PKK156 pKa = 9.58PSGRR160 pKa = 11.84KK161 pKa = 8.62IYY163 pKa = 9.98QVNAMEE169 pKa = 4.56FVNGIWQVKK178 pKa = 9.42CDD180 pKa = 3.92YY181 pKa = 10.52LCPVEE186 pKa = 6.06FNWNDD191 pKa = 3.02NGICVGDD198 pKa = 3.68IDD200 pKa = 5.53IVDD203 pKa = 3.65KK204 pKa = 11.28NGNLIADD211 pKa = 4.13QEE213 pKa = 4.87CKK215 pKa = 10.11VGSYY219 pKa = 9.75FVFNPDD225 pKa = 5.3KK226 pKa = 10.77ILSDD230 pKa = 3.21NGGAYY235 pKa = 7.91GTGGYY240 pKa = 7.67YY241 pKa = 9.17WRR243 pKa = 11.84NFTFAEE249 pKa = 4.89GGEE252 pKa = 4.33AWLSTWDD259 pKa = 4.26KK260 pKa = 11.46KK261 pKa = 11.26DD262 pKa = 3.47LLGG265 pKa = 4.92

Molecular weight: 29.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AX86|A0A4D6AX86_9CAUD Terminase large subunit OS=Streptococcus phage Javan284 OX=2548093 GN=Javan284_0029 PE=4 SV=1
MM1 pKa = 7.35FRR3 pKa = 11.84IRR5 pKa = 11.84TKK7 pKa = 11.08ADD9 pKa = 3.05LSGAEE14 pKa = 4.38RR15 pKa = 11.84KK16 pKa = 10.24VSDD19 pKa = 3.85ANVLRR24 pKa = 11.84GKK26 pKa = 10.18RR27 pKa = 11.84ALANQVLMDD36 pKa = 3.8TDD38 pKa = 3.96KK39 pKa = 11.12YY40 pKa = 11.24IPMKK44 pKa = 10.69GGALRR49 pKa = 11.84ASGQIAIDD57 pKa = 4.26GSAVSWNTVYY67 pKa = 10.96ARR69 pKa = 11.84AQFYY73 pKa = 7.49GTNGIVVFKK82 pKa = 10.68KK83 pKa = 9.31YY84 pKa = 6.41TTPGTGKK91 pKa = 10.22LWYY94 pKa = 9.87DD95 pKa = 3.77KK96 pKa = 11.01SAEE99 pKa = 4.06ANVDD103 pKa = 2.4KK104 pKa = 10.43WKK106 pKa = 10.65RR107 pKa = 11.84VAAKK111 pKa = 10.65GMGFF115 pKa = 3.41

Molecular weight: 12.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11583

49

1316

227.1

25.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.718 ± 1.007

0.553 ± 0.111

6.432 ± 0.414

6.872 ± 0.691

4.256 ± 0.194

6.553 ± 0.553

1.278 ± 0.182

6.63 ± 0.328

8.02 ± 0.49

7.468 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.22

6.233 ± 0.435

2.702 ± 0.154

4.368 ± 0.366

3.591 ± 0.296

6.423 ± 0.433

6.63 ± 0.545

6.423 ± 0.306

1.269 ± 0.149

4.032 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski