Halorubrum pleomorphic virus 6

Taxonomy: Viruses; Monodnaviria; Trapavirae; Saleviricota; Huolimaviricetes; Haloruvirales; Pleolipoviridae; Alphapleolipovirus; Alphapleolipovirus HRPV6

Average proteome isoelectric point is 5.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9ABP7|H9ABP7_9VIRU ORF6 OS=Halorubrum pleomorphic virus 6 OX=1156721 GN=ORF6 PE=4 SV=1
MM1 pKa = 7.55GRR3 pKa = 11.84RR4 pKa = 11.84TFVKK8 pKa = 10.51GLGAAGAASVGLAHH22 pKa = 7.61DD23 pKa = 4.58RR24 pKa = 11.84GAARR28 pKa = 11.84NAEE31 pKa = 4.56AIAPLVGYY39 pKa = 10.29AIGAAAISAVGGIGVGWTLRR59 pKa = 11.84EE60 pKa = 4.03FEE62 pKa = 4.79VVGSDD67 pKa = 4.72DD68 pKa = 3.8PAEE71 pKa = 4.25GLTPDD76 pKa = 3.54VLRR79 pKa = 11.84NQLSDD84 pKa = 3.6SVVKK88 pKa = 10.61RR89 pKa = 11.84KK90 pKa = 10.36SNNQSTMVDD99 pKa = 3.25NQNILDD105 pKa = 4.25GVEE108 pKa = 3.87HH109 pKa = 6.22TAYY112 pKa = 9.83TEE114 pKa = 3.97AKK116 pKa = 8.8IAAIEE121 pKa = 3.95EE122 pKa = 4.43LNAGSSEE129 pKa = 4.28SAVLSAANSAIDD141 pKa = 3.52SYY143 pKa = 10.49EE144 pKa = 3.92TTVRR148 pKa = 11.84TNFYY152 pKa = 10.33KK153 pKa = 10.37SWNEE157 pKa = 3.67TVRR160 pKa = 11.84EE161 pKa = 4.25LEE163 pKa = 4.35AMTQTVIAHH172 pKa = 6.75ADD174 pKa = 3.22VGLSYY179 pKa = 9.8ITDD182 pKa = 3.94FGDD185 pKa = 3.42PRR187 pKa = 11.84FGNLASGTSPNTLKK201 pKa = 10.3DD202 pKa = 3.66TTVSMPDD209 pKa = 3.15GTNFTLLTFRR219 pKa = 11.84HH220 pKa = 4.74NTGWDD225 pKa = 3.43SGNAAYY231 pKa = 10.21SVVEE235 pKa = 4.01YY236 pKa = 10.77NPKK239 pKa = 10.38EE240 pKa = 4.22VVTSTNSNTYY250 pKa = 8.38NTVDD254 pKa = 2.97GTQYY258 pKa = 10.65MKK260 pKa = 10.67FSEE263 pKa = 4.31WNAVEE268 pKa = 4.33TEE270 pKa = 3.86MDD272 pKa = 3.59TVFQNVRR279 pKa = 11.84NGISTWVTNVYY290 pKa = 11.18GDD292 pKa = 3.84VQSGAIEE299 pKa = 4.05ISDD302 pKa = 3.5LVTPRR307 pKa = 11.84EE308 pKa = 4.24RR309 pKa = 11.84ATMMAQEE316 pKa = 4.65EE317 pKa = 4.92GMSQAIADD325 pKa = 5.12LIALNVPVDD334 pKa = 3.84AEE336 pKa = 4.23RR337 pKa = 11.84EE338 pKa = 4.21ATITIQDD345 pKa = 3.56TGATLPGTFALTDD358 pKa = 3.56SSDD361 pKa = 3.78GPLSAGQTYY370 pKa = 10.66DD371 pKa = 3.09PSTFSGDD378 pKa = 3.59VYY380 pKa = 9.75FTADD384 pKa = 3.16MSLVEE389 pKa = 4.75GPWDD393 pKa = 4.62AINSGVDD400 pKa = 2.88GGTITITSEE409 pKa = 4.15PYY411 pKa = 9.2EE412 pKa = 4.25GTAIEE417 pKa = 4.41VTTVEE422 pKa = 4.47SEE424 pKa = 4.64TVSVPAADD432 pKa = 3.35WTDD435 pKa = 3.4NGDD438 pKa = 3.85GTWSYY443 pKa = 11.33DD444 pKa = 3.16ASGDD448 pKa = 4.09LEE450 pKa = 4.42TTITNVDD457 pKa = 3.36SARR460 pKa = 11.84FVSTATEE467 pKa = 4.08TTYY470 pKa = 10.1DD471 pKa = 3.54TLQLKK476 pKa = 10.21GAFTVDD482 pKa = 2.98KK483 pKa = 10.64LVNKK487 pKa = 10.19QSGEE491 pKa = 4.3EE492 pKa = 4.01VSSTSFTSSEE502 pKa = 3.98PQTDD506 pKa = 3.34SNYY509 pKa = 8.7ITQDD513 pKa = 2.37EE514 pKa = 4.42WDD516 pKa = 3.49QLEE519 pKa = 4.23QQNKK523 pKa = 8.28EE524 pKa = 4.17LIEE527 pKa = 4.64KK528 pKa = 10.22YY529 pKa = 9.73EE530 pKa = 3.97QSQSGGGLDD539 pKa = 4.92LGGLDD544 pKa = 3.42MFGVPGEE551 pKa = 4.16MVAVGAAAVIGFLMLGNNN569 pKa = 3.91

Molecular weight: 60.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9ABP4|H9ABP4_9VIRU ORF3 OS=Halorubrum pleomorphic virus 6 OX=1156721 GN=ORF3 PE=4 SV=1
MM1 pKa = 6.31TTCPLTTHH9 pKa = 6.29YY10 pKa = 11.43ADD12 pKa = 3.46GRR14 pKa = 11.84RR15 pKa = 11.84PEE17 pKa = 4.63ADD19 pKa = 3.03ANRR22 pKa = 11.84SADD25 pKa = 3.67EE26 pKa = 4.04TRR28 pKa = 11.84SPGGEE33 pKa = 3.41RR34 pKa = 11.84AASQLLEE41 pKa = 3.98SAAAQEE47 pKa = 4.5MKK49 pKa = 10.57TPGTDD54 pKa = 3.02TVRR57 pKa = 11.84KK58 pKa = 8.29PGSPRR63 pKa = 11.84SLNPEE68 pKa = 3.49RR69 pKa = 11.84RR70 pKa = 11.84AKK72 pKa = 10.16RR73 pKa = 11.84AEE75 pKa = 3.82GNRR78 pKa = 11.84DD79 pKa = 2.6GSRR82 pKa = 11.84RR83 pKa = 11.84GRR85 pKa = 11.84QPGTASWPRR94 pKa = 11.84PEE96 pKa = 5.07SLDD99 pKa = 3.68LRR101 pKa = 11.84AAEE104 pKa = 4.63CSSCKK109 pKa = 9.18TKK111 pKa = 10.76RR112 pKa = 11.84SDD114 pKa = 2.8TRR116 pKa = 11.84ARR118 pKa = 11.84LEE120 pKa = 4.19AGATRR125 pKa = 11.84LL126 pKa = 3.69

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

2676

74

655

267.6

29.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.118 ± 0.686

0.71 ± 0.299

8.969 ± 1.466

6.577 ± 0.781

3.102 ± 0.45

8.034 ± 0.52

1.682 ± 0.508

3.7 ± 0.377

2.99 ± 0.584

8.819 ± 0.807

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.644 ± 0.217

3.363 ± 0.544

4.41 ± 0.47

2.99 ± 0.35

6.988 ± 1.334

7.549 ± 1.046

7.735 ± 0.963

7.362 ± 0.836

1.719 ± 0.175

2.541 ± 0.371
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski