Bifidobacterium indicum LMG 11587 = DSM 20214
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1350 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A087VTU1|A0A087VTU1_9BIFI Putative sugar ABC transporter substrate-binding protein OS=Bifidobacterium indicum LMG 11587 = DSM 20214 OX=1341694 GN=BINDI_0458 PE=4 SV=1
MM1 pKa = 7.13 AQDD4 pKa = 3.51 YY5 pKa = 10.09 DD6 pKa = 3.88 SPRR9 pKa = 11.84 DD10 pKa = 3.45 KK11 pKa = 11.62 DD12 pKa = 3.48 EE13 pKa = 5.66 DD14 pKa = 3.93 EE15 pKa = 5.11 EE16 pKa = 4.52 SLQEE20 pKa = 4.58 LGKK23 pKa = 9.23 TSQDD27 pKa = 2.96 AGADD31 pKa = 3.61 LDD33 pKa = 4.45 DD34 pKa = 5.6 DD35 pKa = 4.49 EE36 pKa = 5.33 NAIAEE41 pKa = 4.35 DD42 pKa = 4.23 YY43 pKa = 9.11 EE44 pKa = 5.05 LPGADD49 pKa = 4.92 LSNEE53 pKa = 4.01 DD54 pKa = 3.61 SSVTVIPMQGDD65 pKa = 3.56 EE66 pKa = 5.63 FICSSCFLVKK76 pKa = 10.31 HH77 pKa = 6.42 RR78 pKa = 11.84 SQLAYY83 pKa = 6.59 TTPDD87 pKa = 3.21 GQPVCKK93 pKa = 10.14 EE94 pKa = 3.94 CAAA97 pKa = 4.21
Molecular weight: 10.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.668
IPC_protein 3.656
Toseland 3.439
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.35
Solomon 3.63
Lehninger 3.592
Nozaki 3.77
DTASelect 3.999
Thurlkill 3.503
EMBOSS 3.605
Sillero 3.783
Patrickios 1.837
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.712
Protein with the highest isoelectric point:
>tr|A0A087VW84|A0A087VW84_9BIFI FAD:protein FMN transferase OS=Bifidobacterium indicum LMG 11587 = DSM 20214 OX=1341694 GN=BINDI_1334 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 4.79 MKK15 pKa = 9.36 HH16 pKa = 5.81 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 ALINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.08 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1350
0
1350
507539
29
2315
376.0
41.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.84 ± 0.065
0.841 ± 0.018
6.727 ± 0.061
5.536 ± 0.078
3.195 ± 0.043
8.682 ± 0.084
2.093 ± 0.029
5.168 ± 0.054
3.687 ± 0.058
9.262 ± 0.083
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.606 ± 0.036
2.948 ± 0.041
4.955 ± 0.048
4.014 ± 0.044
6.435 ± 0.063
6.571 ± 0.056
5.976 ± 0.088
7.556 ± 0.062
1.364 ± 0.03
2.543 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here