Streptomyces sp. NRRL F-4428

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5663 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F0H0W8|A0A0F0H0W8_9ACTN SnoaL-like domain-containing protein OS=Streptomyces sp. NRRL F-4428 OX=1609137 GN=UK14_16175 PE=4 SV=1
MM1 pKa = 6.51ITMYY5 pKa = 10.56AWPSTADD12 pKa = 3.82GPDD15 pKa = 4.02ALPMVHH21 pKa = 6.51FTTDD25 pKa = 2.99EE26 pKa = 4.0QAGGEE31 pKa = 4.22VTPDD35 pKa = 3.43GTPVWLFDD43 pKa = 3.24TAIRR47 pKa = 11.84EE48 pKa = 4.45GGWAPFADD56 pKa = 4.01FEE58 pKa = 4.71GWSTPAADD66 pKa = 2.84WRR68 pKa = 11.84ALYY71 pKa = 10.21RR72 pKa = 11.84RR73 pKa = 11.84EE74 pKa = 4.13DD75 pKa = 3.62DD76 pKa = 4.02FLAVTGPGACQGWYY90 pKa = 9.71EE91 pKa = 4.13GSLGADD97 pKa = 3.83AEE99 pKa = 4.54WVAAAAAQQSVVLLAAPVQHH119 pKa = 6.89PSLYY123 pKa = 10.35GYY125 pKa = 10.53AVEE128 pKa = 4.87AGAGFALLVPLLVVV142 pKa = 3.87

Molecular weight: 15.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F0HGE9|A0A0F0HGE9_9ACTN Membrane protein OS=Streptomyces sp. NRRL F-4428 OX=1609137 GN=UK14_00610 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.86GRR40 pKa = 11.84SALSAA45 pKa = 3.8

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5663

0

5663

1785054

25

3685

315.2

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.187 ± 0.053

0.796 ± 0.008

5.852 ± 0.027

5.784 ± 0.03

2.733 ± 0.017

9.64 ± 0.037

2.251 ± 0.016

3.076 ± 0.021

2.207 ± 0.027

10.303 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.776 ± 0.014

1.737 ± 0.019

6.198 ± 0.031

2.734 ± 0.02

7.976 ± 0.038

4.859 ± 0.023

5.889 ± 0.024

8.436 ± 0.027

1.496 ± 0.014

2.072 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski