Amino acid dipepetide frequency for Lysobacter enzymogenes

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.808AlaAla: 23.808 ± 0.238	1.384AlaCys: 1.384 ± 0.031	7.931AlaAsp: 7.931 ± 0.071	6.753AlaGlu: 6.753 ± 0.087	4.337AlaPhe: 4.337 ± 0.054	12.473AlaGly: 12.473 ± 0.112	2.779AlaHis: 2.779 ± 0.052	5.171AlaIle: 5.171 ± 0.059	3.519AlaLys: 3.519 ± 0.071	16.741AlaLeu: 16.741 ± 0.171	3.055AlaMet: 3.055 ± 0.052	2.935AlaAsn: 2.935 ± 0.048	8.526AlaPro: 8.526 ± 0.114	6.77AlaGln: 6.77 ± 0.089	11.542AlaArg: 11.542 ± 0.121	6.963AlaSer: 6.963 ± 0.077	6.149AlaThr: 6.149 ± 0.078	8.972AlaVal: 8.972 ± 0.091	2.128AlaTrp: 2.128 ± 0.039	2.769AlaTyr: 2.769 ± 0.053	0.005AlaXaa: 0.005 ± 0.002
Cys	1.391CysAla: 1.391 ± 0.034	0.119CysCys: 0.119 ± 0.008	0.518CysAsp: 0.518 ± 0.016	0.499CysGlu: 0.499 ± 0.018	0.289CysPhe: 0.289 ± 0.013	0.989CysGly: 0.989 ± 0.028	0.183CysHis: 0.183 ± 0.012	0.303CysIle: 0.303 ± 0.015	0.204CysLys: 0.204 ± 0.011	0.806CysLeu: 0.806 ± 0.023	0.148CysMet: 0.148 ± 0.009	0.226CysAsn: 0.226 ± 0.012	0.43CysPro: 0.43 ± 0.021	0.225CysGln: 0.225 ± 0.013	0.657CysArg: 0.657 ± 0.022	0.486CysSer: 0.486 ± 0.018	0.467CysThr: 0.467 ± 0.041	0.646CysVal: 0.646 ± 0.017	0.127CysTrp: 0.127 ± 0.009	0.215CysTyr: 0.215 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	9.453AspAla: 9.453 ± 0.112	0.496AspCys: 0.496 ± 0.017	3.431AspAsp: 3.431 ± 0.048	3.29AspGlu: 3.29 ± 0.051	2.016AspPhe: 2.016 ± 0.041	6.475AspGly: 6.475 ± 0.076	1.058AspHis: 1.058 ± 0.025	1.95AspIle: 1.95 ± 0.034	1.591AspLys: 1.591 ± 0.045	5.524AspLeu: 5.524 ± 0.06	0.874AspMet: 0.874 ± 0.024	1.362AspAsn: 1.362 ± 0.031	3.884AspPro: 3.884 ± 0.056	1.652AspGln: 1.652 ± 0.033	4.349AspArg: 4.349 ± 0.056	2.77AspSer: 2.77 ± 0.041	2.535AspThr: 2.535 ± 0.048	3.515AspVal: 3.515 ± 0.052	1.105AspTrp: 1.105 ± 0.027	1.827AspTyr: 1.827 ± 0.036	0.001AspXaa: 0.001 ± 0.001
Glu	6.581GluAla: 6.581 ± 0.088	0.388GluCys: 0.388 ± 0.017	2.47GluAsp: 2.47 ± 0.046	2.08GluGlu: 2.08 ± 0.043	1.799GluPhe: 1.799 ± 0.03	3.487GluGly: 3.487 ± 0.051	1.315GluHis: 1.315 ± 0.029	2.256GluIle: 2.256 ± 0.043	1.268GluLys: 1.268 ± 0.034	6.376GluLeu: 6.376 ± 0.098	0.887GluMet: 0.887 ± 0.025	1.15GluAsn: 1.15 ± 0.027	2.774GluPro: 2.774 ± 0.044	2.399GluGln: 2.399 ± 0.041	6.043GluArg: 6.043 ± 0.075	2.443GluSer: 2.443 ± 0.044	2.462GluThr: 2.462 ± 0.04	3.275GluVal: 3.275 ± 0.049	0.695GluTrp: 0.695 ± 0.02	1.152GluTyr: 1.152 ± 0.035	0.001GluXaa: 0.001 ± 0.001
Phe	4.939PheAla: 4.939 ± 0.066	0.334PheCys: 0.334 ± 0.013	2.819PheAsp: 2.819 ± 0.054	2.036PheGlu: 2.036 ± 0.04	1.128PhePhe: 1.128 ± 0.027	3.353PheGly: 3.353 ± 0.056	0.658PheHis: 0.658 ± 0.021	0.961PheIle: 0.961 ± 0.024	0.909PheLys: 0.909 ± 0.028	2.695PheLeu: 2.695 ± 0.044	0.556PheMet: 0.556 ± 0.02	0.989PheAsn: 0.989 ± 0.025	1.334PhePro: 1.334 ± 0.029	0.931PheGln: 0.931 ± 0.025	2.005PheArg: 2.005 ± 0.038	1.774PheSer: 1.774 ± 0.037	1.339PheThr: 1.339 ± 0.031	2.678PheVal: 2.678 ± 0.042	0.474PheTrp: 0.474 ± 0.02	0.851PheTyr: 0.851 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	11.192GlyAla: 11.192 ± 0.121	0.865GlyCys: 0.865 ± 0.025	5.256GlyAsp: 5.256 ± 0.065	5.393GlyGlu: 5.393 ± 0.067	3.374GlyPhe: 3.374 ± 0.053	8.12GlyGly: 8.12 ± 0.141	1.898GlyHis: 1.898 ± 0.034	3.3GlyIle: 3.3 ± 0.051	2.809GlyLys: 2.809 ± 0.054	8.847GlyLeu: 8.847 ± 0.095	1.798GlyMet: 1.798 ± 0.038	2.434GlyAsn: 2.434 ± 0.06	3.444GlyPro: 3.444 ± 0.054	3.133GlyGln: 3.133 ± 0.052	7.113GlyArg: 7.113 ± 0.084	4.675GlySer: 4.675 ± 0.072	3.968GlyThr: 3.968 ± 0.103	6.0GlyVal: 6.0 ± 0.068	1.661GlyTrp: 1.661 ± 0.035	2.633GlyTyr: 2.633 ± 0.044	0.001GlyXaa: 0.001 ± 0.001
His	2.903HisAla: 2.903 ± 0.046	0.245HisCys: 0.245 ± 0.013	1.235HisAsp: 1.235 ± 0.029	1.072HisGlu: 1.072 ± 0.031	0.709HisPhe: 0.709 ± 0.023	2.219HisGly: 2.219 ± 0.039	0.54HisHis: 0.54 ± 0.022	0.607HisIle: 0.607 ± 0.019	0.388HisLys: 0.388 ± 0.015	1.984HisLeu: 1.984 ± 0.039	0.332HisMet: 0.332 ± 0.016	0.438HisAsn: 0.438 ± 0.016	1.382HisPro: 1.382 ± 0.031	0.562HisGln: 0.562 ± 0.018	1.819HisArg: 1.819 ± 0.034	1.066HisSer: 1.066 ± 0.023	0.826HisThr: 0.826 ± 0.024	1.264HisVal: 1.264 ± 0.03	0.44HisTrp: 0.44 ± 0.016	0.621HisTyr: 0.621 ± 0.021	0.001HisXaa: 0.001 ± 0.001
Ile	6.282IleAla: 6.282 ± 0.07	0.326IleCys: 0.326 ± 0.016	3.161IleAsp: 3.161 ± 0.043	2.911IleGlu: 2.911 ± 0.048	0.906IlePhe: 0.906 ± 0.026	4.178IleGly: 4.178 ± 0.05	0.706IleHis: 0.706 ± 0.019	0.768IleIle: 0.768 ± 0.024	1.019IleLys: 1.019 ± 0.031	2.468IleLeu: 2.468 ± 0.04	0.424IleMet: 0.424 ± 0.018	1.08IleAsn: 1.08 ± 0.028	1.635IlePro: 1.635 ± 0.036	1.022IleGln: 1.022 ± 0.023	2.383IleArg: 2.383 ± 0.04	1.752IleSer: 1.752 ± 0.037	1.508IleThr: 1.508 ± 0.049	3.147IleVal: 3.147 ± 0.048	0.386IleTrp: 0.386 ± 0.015	0.774IleTyr: 0.774 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	3.066LysAla: 3.066 ± 0.056	0.138LysCys: 0.138 ± 0.01	1.299LysAsp: 1.299 ± 0.035	1.008LysGlu: 1.008 ± 0.03	0.772LysPhe: 0.772 ± 0.025	1.75LysGly: 1.75 ± 0.038	0.559LysHis: 0.559 ± 0.018	1.165LysIle: 1.165 ± 0.03	0.941LysLys: 0.941 ± 0.046	2.909LysLeu: 2.909 ± 0.049	0.506LysMet: 0.506 ± 0.018	0.689LysAsn: 0.689 ± 0.025	2.041LysPro: 2.041 ± 0.041	1.123LysGln: 1.123 ± 0.03	2.25LysArg: 2.25 ± 0.042	1.435LysSer: 1.435 ± 0.035	1.491LysThr: 1.491 ± 0.04	1.877LysVal: 1.877 ± 0.044	0.296LysTrp: 0.296 ± 0.014	0.607LysTyr: 0.607 ± 0.02	0.001LysXaa: 0.001 ± 0.001
Leu	15.711LeuAla: 15.711 ± 0.162	0.989LeuCys: 0.989 ± 0.025	6.933LeuAsp: 6.933 ± 0.102	4.912LeuGlu: 4.912 ± 0.06	3.229LeuPhe: 3.229 ± 0.049	8.599LeuGly: 8.599 ± 0.087	2.206LeuHis: 2.206 ± 0.043	3.832LeuIle: 3.832 ± 0.056	2.768LeuLys: 2.768 ± 0.051	10.829LeuLeu: 10.829 ± 0.132	1.797LeuMet: 1.797 ± 0.036	2.352LeuAsn: 2.352 ± 0.046	6.374LeuPro: 6.374 ± 0.097	3.836LeuGln: 3.836 ± 0.061	9.838LeuArg: 9.838 ± 0.108	6.316LeuSer: 6.316 ± 0.075	4.475LeuThr: 4.475 ± 0.065	6.375LeuVal: 6.375 ± 0.075	1.451LeuTrp: 1.451 ± 0.033	2.179LeuTyr: 2.179 ± 0.039	0.001LeuXaa: 0.001 ± 0.001
Met	2.237MetAla: 2.237 ± 0.04	0.126MetCys: 0.126 ± 0.01	0.909MetAsp: 0.909 ± 0.025	0.682MetGlu: 0.682 ± 0.02	0.558MetPhe: 0.558 ± 0.02	1.203MetGly: 1.203 ± 0.029	0.385MetHis: 0.385 ± 0.016	0.754MetIle: 0.754 ± 0.022	0.641MetLys: 0.641 ± 0.021	1.958MetLeu: 1.958 ± 0.038	0.358MetMet: 0.358 ± 0.016	0.675MetAsn: 0.675 ± 0.019	1.227MetPro: 1.227 ± 0.029	0.781MetGln: 0.781 ± 0.023	1.702MetArg: 1.702 ± 0.036	1.453MetSer: 1.453 ± 0.032	1.136MetThr: 1.136 ± 0.027	1.133MetVal: 1.133 ± 0.032	0.168MetTrp: 0.168 ± 0.01	0.348MetTyr: 0.348 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.406AsnAla: 3.406 ± 0.059	0.229AsnCys: 0.229 ± 0.016	1.408AsnAsp: 1.408 ± 0.033	1.145AsnGlu: 1.145 ± 0.033	0.876AsnPhe: 0.876 ± 0.026	2.516AsnGly: 2.516 ± 0.052	0.467AsnHis: 0.467 ± 0.015	0.925AsnIle: 0.925 ± 0.027	0.61AsnLys: 0.61 ± 0.022	2.392AsnLeu: 2.392 ± 0.052	0.347AsnMet: 0.347 ± 0.015	0.746AsnAsn: 0.746 ± 0.033	1.771AsnPro: 1.771 ± 0.035	0.818AsnGln: 0.818 ± 0.021	1.75AsnArg: 1.75 ± 0.039	1.169AsnSer: 1.169 ± 0.034	1.198AsnThr: 1.198 ± 0.033	1.687AsnVal: 1.687 ± 0.042	0.397AsnTrp: 0.397 ± 0.015	0.744AsnTyr: 0.744 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	9.222ProAla: 9.222 ± 0.124	0.363ProCys: 0.363 ± 0.013	3.491ProAsp: 3.491 ± 0.051	2.923ProGlu: 2.923 ± 0.043	1.579ProPhe: 1.579 ± 0.03	5.142ProGly: 5.142 ± 0.056	1.088ProHis: 1.088 ± 0.027	1.77ProIle: 1.77 ± 0.035	1.418ProLys: 1.418 ± 0.031	5.509ProLeu: 5.509 ± 0.089	1.195ProMet: 1.195 ± 0.029	1.369ProAsn: 1.369 ± 0.03	3.333ProPro: 3.333 ± 0.07	2.48ProGln: 2.48 ± 0.043	4.079ProArg: 4.079 ± 0.058	2.869ProSer: 2.869 ± 0.046	2.48ProThr: 2.48 ± 0.043	3.942ProVal: 3.942 ± 0.055	0.844ProTrp: 0.844 ± 0.024	1.165ProTyr: 1.165 ± 0.028	0.001ProXaa: 0.001 ± 0.001
Gln	5.989GlnAla: 5.989 ± 0.081	0.299GlnCys: 0.299 ± 0.013	1.622GlnAsp: 1.622 ± 0.037	1.27GlnGlu: 1.27 ± 0.033	1.096GlnPhe: 1.096 ± 0.03	2.781GlnGly: 2.781 ± 0.042	0.768GlnHis: 0.768 ± 0.024	1.507GlnIle: 1.507 ± 0.027	0.745GlnLys: 0.745 ± 0.02	3.907GlnLeu: 3.907 ± 0.055	0.672GlnMet: 0.672 ± 0.019	0.743GlnAsn: 0.743 ± 0.022	2.369GlnPro: 2.369 ± 0.039	1.746GlnGln: 1.746 ± 0.041	4.301GlnArg: 4.301 ± 0.063	1.791GlnSer: 1.791 ± 0.031	1.727GlnThr: 1.727 ± 0.038	2.764GlnVal: 2.764 ± 0.042	0.722GlnTrp: 0.722 ± 0.022	0.821GlnTyr: 0.821 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	10.815ArgAla: 10.815 ± 0.106	0.768ArgCys: 0.768 ± 0.023	4.982ArgAsp: 4.982 ± 0.06	5.314ArgGlu: 5.314 ± 0.073	2.982ArgPhe: 2.982 ± 0.046	6.343ArgGly: 6.343 ± 0.078	1.91ArgHis: 1.91 ± 0.035	3.98ArgIle: 3.98 ± 0.048	2.113ArgLys: 2.113 ± 0.049	9.177ArgLeu: 9.177 ± 0.104	1.876ArgMet: 1.876 ± 0.037	2.029ArgAsn: 2.029 ± 0.037	4.091ArgPro: 4.091 ± 0.059	2.896ArgGln: 2.896 ± 0.049	7.355ArgArg: 7.355 ± 0.116	4.238ArgSer: 4.238 ± 0.049	3.272ArgThr: 3.272 ± 0.046	5.356ArgVal: 5.356 ± 0.056	1.568ArgTrp: 1.568 ± 0.034	2.537ArgTyr: 2.537 ± 0.05	0.005ArgXaa: 0.005 ± 0.002
Ser	7.296SerAla: 7.296 ± 0.084	0.423SerCys: 0.423 ± 0.018	2.849SerAsp: 2.849 ± 0.042	2.462SerGlu: 2.462 ± 0.038	1.89SerPhe: 1.89 ± 0.037	5.42SerGly: 5.42 ± 0.07	1.081SerHis: 1.081 ± 0.024	1.965SerIle: 1.965 ± 0.029	1.384SerLys: 1.384 ± 0.031	5.467SerLeu: 5.467 ± 0.061	0.932SerMet: 0.932 ± 0.024	1.363SerAsn: 1.363 ± 0.033	2.915SerPro: 2.915 ± 0.045	1.804SerGln: 1.804 ± 0.035	3.832SerArg: 3.832 ± 0.057	2.798SerSer: 2.798 ± 0.053	2.377SerThr: 2.377 ± 0.051	3.742SerVal: 3.742 ± 0.063	0.803SerTrp: 0.803 ± 0.023	1.418SerTyr: 1.418 ± 0.037	0.002SerXaa: 0.002 ± 0.001
Thr	5.953ThrAla: 5.953 ± 0.07	0.389ThrCys: 0.389 ± 0.026	2.384ThrAsp: 2.384 ± 0.049	1.795ThrGlu: 1.795 ± 0.036	1.401ThrPhe: 1.401 ± 0.035	4.222ThrGly: 4.222 ± 0.076	0.92ThrHis: 0.92 ± 0.023	1.734ThrIle: 1.734 ± 0.044	0.873ThrLys: 0.873 ± 0.026	5.473ThrLeu: 5.473 ± 0.085	0.759ThrMet: 0.759 ± 0.024	0.983ThrAsn: 0.983 ± 0.04	3.124ThrPro: 3.124 ± 0.046	1.609ThrGln: 1.609 ± 0.038	3.126ThrArg: 3.126 ± 0.048	2.133ThrSer: 2.133 ± 0.042	2.26ThrThr: 2.26 ± 0.102	3.934ThrVal: 3.934 ± 0.081	0.605ThrTrp: 0.605 ± 0.018	1.201ThrTyr: 1.201 ± 0.052	0.001ThrXaa: 0.001 ± 0.001
Val	9.898ValAla: 9.898 ± 0.096	0.655ValCys: 0.655 ± 0.021	4.037ValAsp: 4.037 ± 0.052	4.069ValGlu: 4.069 ± 0.057	2.331ValPhe: 2.331 ± 0.041	5.471ValGly: 5.471 ± 0.062	1.356ValHis: 1.356 ± 0.032	2.534ValIle: 2.534 ± 0.041	1.699ValLys: 1.699 ± 0.033	7.184ValLeu: 7.184 ± 0.075	1.199ValMet: 1.199 ± 0.028	1.754ValAsn: 1.754 ± 0.044	3.613ValPro: 3.613 ± 0.052	2.266ValGln: 2.266 ± 0.04	5.424ValArg: 5.424 ± 0.063	3.763ValSer: 3.763 ± 0.05	3.047ValThr: 3.047 ± 0.079	5.178ValVal: 5.178 ± 0.073	0.938ValTrp: 0.938 ± 0.025	1.634ValTyr: 1.634 ± 0.04	0.001ValXaa: 0.001 ± 0.001
Trp	1.404TrpAla: 1.404 ± 0.029	0.151TrpCys: 0.151 ± 0.01	0.722TrpAsp: 0.722 ± 0.025	0.536TrpGlu: 0.536 ± 0.02	0.593TrpPhe: 0.593 ± 0.02	0.929TrpGly: 0.929 ± 0.024	0.336TrpHis: 0.336 ± 0.014	0.693TrpIle: 0.693 ± 0.021	0.448TrpLys: 0.448 ± 0.019	2.19TrpLeu: 2.19 ± 0.046	0.365TrpMet: 0.365 ± 0.015	0.564TrpAsn: 0.564 ± 0.018	0.812TrpPro: 0.812 ± 0.023	0.733TrpGln: 0.733 ± 0.021	1.674TrpArg: 1.674 ± 0.034	1.007TrpSer: 1.007 ± 0.025	0.837TrpThr: 0.837 ± 0.026	0.898TrpVal: 0.898 ± 0.025	0.321TrpTrp: 0.321 ± 0.015	0.344TrpTyr: 0.344 ± 0.015	0.001TrpXaa: 0.001 ± 0.001
Tyr	3.159TyrAla: 3.159 ± 0.051	0.241TyrCys: 0.241 ± 0.013	1.695TyrAsp: 1.695 ± 0.086	1.15TyrGlu: 1.15 ± 0.027	0.897TyrPhe: 0.897 ± 0.024	2.242TyrGly: 2.242 ± 0.039	0.442TyrHis: 0.442 ± 0.015	0.661TyrIle: 0.661 ± 0.02	0.575TyrLys: 0.575 ± 0.02	2.564TyrLeu: 2.564 ± 0.034	0.353TyrMet: 0.353 ± 0.014	0.716TyrAsn: 0.716 ± 0.03	1.183TyrPro: 1.183 ± 0.029	0.856TyrGln: 0.856 ± 0.023	2.365TyrArg: 2.365 ± 0.04	1.318TyrSer: 1.318 ± 0.037	1.324TyrThr: 1.324 ± 0.066	1.669TyrVal: 1.669 ± 0.035	0.429TyrTrp: 0.429 ± 0.015	0.684TyrTyr: 0.684 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.005XaaAla: 0.005 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.002XaaAsp: 0.002 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.003XaaSer: 0.003 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	1.014XaaXaa: 1.014 ± 0.3

Statistics based on 5514 proteins (1754100 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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