Faeces associated gemycircularvirus 7

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus malas1; Mallard associated gemycircularvirus 1

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1YRX6|T1YRX6_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 7 OX=1391036 PE=3 SV=1
MM1 pKa = 7.64SRR3 pKa = 11.84QFCLKK8 pKa = 10.02DD9 pKa = 3.36AKK11 pKa = 10.79YY12 pKa = 10.93CLLTYY17 pKa = 9.15PQIPEE22 pKa = 3.8TEE24 pKa = 4.1AYY26 pKa = 7.96EE27 pKa = 4.39FPEE30 pKa = 4.29LASEE34 pKa = 4.49LFSSEE39 pKa = 4.45RR40 pKa = 11.84VDD42 pKa = 3.25ANYY45 pKa = 10.53VIGRR49 pKa = 11.84EE50 pKa = 3.9LHH52 pKa = 6.73ADD54 pKa = 3.36GGYY57 pKa = 9.17TSHH60 pKa = 7.21CFLDD64 pKa = 4.41FGRR67 pKa = 11.84KK68 pKa = 8.49FSSRR72 pKa = 11.84DD73 pKa = 3.11TRR75 pKa = 11.84IFDD78 pKa = 3.7IQGHH82 pKa = 5.87HH83 pKa = 6.82PNIEE87 pKa = 4.16RR88 pKa = 11.84VGRR91 pKa = 11.84TPRR94 pKa = 11.84TAYY97 pKa = 10.8NYY99 pKa = 7.81TIKK102 pKa = 11.17DD103 pKa = 3.28NDD105 pKa = 3.32VVARR109 pKa = 11.84SGEE112 pKa = 4.08YY113 pKa = 10.09SGPPEE118 pKa = 4.15NNNSRR123 pKa = 11.84EE124 pKa = 4.05RR125 pKa = 11.84QSGNDD130 pKa = 3.61TSSDD134 pKa = 2.97WATILCAEE142 pKa = 4.39SRR144 pKa = 11.84DD145 pKa = 4.06EE146 pKa = 6.23FFDD149 pKa = 3.72LCKK152 pKa = 10.47SLQPRR157 pKa = 11.84SLACSFLSLTRR168 pKa = 11.84YY169 pKa = 10.08ADD171 pKa = 2.87WRR173 pKa = 11.84YY174 pKa = 10.14RR175 pKa = 11.84PVPTPYY181 pKa = 10.07QHH183 pKa = 7.49PDD185 pKa = 2.69DD186 pKa = 3.67WSFNLEE192 pKa = 4.11SHH194 pKa = 6.62SVLLDD199 pKa = 3.31WVDD202 pKa = 3.17EE203 pKa = 4.28SLRR206 pKa = 11.84GGQDD210 pKa = 2.55RR211 pKa = 11.84SLVMYY216 pKa = 10.89GEE218 pKa = 4.24TRR220 pKa = 11.84LGKK223 pKa = 7.62TVWARR228 pKa = 11.84SLGPHH233 pKa = 7.21LYY235 pKa = 10.24FCGLYY240 pKa = 9.92SYY242 pKa = 11.19KK243 pKa = 10.15EE244 pKa = 3.72ASRR247 pKa = 11.84AHH249 pKa = 5.4EE250 pKa = 4.08AEE252 pKa = 4.0YY253 pKa = 10.54AIFDD257 pKa = 4.38DD258 pKa = 4.32LQGGIKK264 pKa = 10.08FFHH267 pKa = 6.68GFKK270 pKa = 10.25NWLGAQQEE278 pKa = 4.28FQIKK282 pKa = 9.86GLYY285 pKa = 9.46RR286 pKa = 11.84DD287 pKa = 4.08PEE289 pKa = 4.16LLKK292 pKa = 10.23WGKK295 pKa = 9.65PSIWCSNTDD304 pKa = 3.12PRR306 pKa = 11.84QDD308 pKa = 3.41LDD310 pKa = 3.7YY311 pKa = 11.5SDD313 pKa = 5.27RR314 pKa = 11.84CWLEE318 pKa = 3.95GNCVFVEE325 pKa = 4.48VTTSLIGG332 pKa = 3.4

Molecular weight: 38.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1YRX6|T1YRX6_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 7 OX=1391036 PE=3 SV=1
MM1 pKa = 7.78AYY3 pKa = 8.88TRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84YY8 pKa = 6.31GTRR11 pKa = 11.84YY12 pKa = 8.91ARR14 pKa = 11.84RR15 pKa = 11.84GVRR18 pKa = 11.84RR19 pKa = 11.84GSRR22 pKa = 11.84RR23 pKa = 11.84TSTRR27 pKa = 11.84GYY29 pKa = 8.82VRR31 pKa = 11.84KK32 pKa = 9.58RR33 pKa = 11.84ANKK36 pKa = 7.65YY37 pKa = 8.0RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84TGVSRR47 pKa = 11.84KK48 pKa = 9.58RR49 pKa = 11.84ILNITSTKK57 pKa = 10.27KK58 pKa = 10.08ADD60 pKa = 3.47HH61 pKa = 6.8MINANKK67 pKa = 9.67DD68 pKa = 3.65DD69 pKa = 4.41PVTPVNPLSGLGPTIVNPANGVTQCYY95 pKa = 9.78AWIATARR102 pKa = 11.84DD103 pKa = 3.61NTNTPGVPAGGVDD116 pKa = 3.64DD117 pKa = 4.38SSRR120 pKa = 11.84TADD123 pKa = 3.17VCYY126 pKa = 10.14MRR128 pKa = 11.84GLKK131 pKa = 9.77EE132 pKa = 4.13RR133 pKa = 11.84IGLEE137 pKa = 3.99SSSNLPFQWRR147 pKa = 11.84RR148 pKa = 11.84ICFTFKK154 pKa = 10.46GQEE157 pKa = 3.73ILRR160 pKa = 11.84DD161 pKa = 3.65RR162 pKa = 11.84TSTAVGSLYY171 pKa = 10.62QEE173 pKa = 4.44AAPPGWMRR181 pKa = 11.84TTTHH185 pKa = 5.51IQGLNVTSNFVYY197 pKa = 10.81DD198 pKa = 3.83NLEE201 pKa = 4.27TILFRR206 pKa = 11.84GVKK209 pKa = 9.78NLDD212 pKa = 3.18WFDD215 pKa = 3.64EE216 pKa = 4.24MNAQVDD222 pKa = 4.49RR223 pKa = 11.84YY224 pKa = 10.13RR225 pKa = 11.84VDD227 pKa = 3.44LKK229 pKa = 10.8YY230 pKa = 11.17DD231 pKa = 4.45RR232 pKa = 11.84IRR234 pKa = 11.84SFQSGNDD241 pKa = 3.12VGLLRR246 pKa = 11.84KK247 pKa = 9.74INMWHH252 pKa = 6.83PMNKK256 pKa = 9.21NLEE259 pKa = 4.31YY260 pKa = 11.07NSDD263 pKa = 3.34EE264 pKa = 4.41SAGGRR269 pKa = 11.84LEE271 pKa = 4.74SVLSSNTKK279 pKa = 10.52GSMGDD284 pKa = 3.62YY285 pKa = 10.66YY286 pKa = 11.41VIDD289 pKa = 3.82YY290 pKa = 10.52FRR292 pKa = 11.84FSAFATINDD301 pKa = 5.0AIAFTPEE308 pKa = 3.24ACLYY312 pKa = 7.41WHH314 pKa = 6.72EE315 pKa = 4.24RR316 pKa = 3.51

Molecular weight: 36.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

648

316

332

324.0

37.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.864 ± 0.548

2.16 ± 0.628

6.79 ± 0.546

5.247 ± 1.24

4.475 ± 0.698

7.253 ± 0.24

2.006 ± 0.52

4.167 ± 0.407

3.858 ± 0.18

7.562 ± 1.087

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.543 ± 0.694

5.247 ± 1.204

4.321 ± 0.367

2.932 ± 0.503

9.722 ± 1.617

8.025 ± 0.746

6.327 ± 1.112

4.938 ± 0.754

2.315 ± 0.292

5.247 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski