Amino acid dipepetide frequency for Clostridium estertheticum subsp. estertheticum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.12AlaAla: 4.12 ± 0.081	0.71AlaCys: 0.71 ± 0.027	2.633AlaAsp: 2.633 ± 0.049	3.075AlaGlu: 3.075 ± 0.051	2.506AlaPhe: 2.506 ± 0.043	3.961AlaGly: 3.961 ± 0.068	0.803AlaHis: 0.803 ± 0.025	5.998AlaIle: 5.998 ± 0.081	4.848AlaLys: 4.848 ± 0.068	5.668AlaLeu: 5.668 ± 0.079	1.655AlaMet: 1.655 ± 0.036	2.81AlaAsn: 2.81 ± 0.047	1.396AlaPro: 1.396 ± 0.035	1.656AlaGln: 1.656 ± 0.039	1.898AlaArg: 1.898 ± 0.045	3.742AlaSer: 3.742 ± 0.066	3.268AlaThr: 3.268 ± 0.062	4.168AlaVal: 4.168 ± 0.069	0.39AlaTrp: 0.39 ± 0.018	2.057AlaTyr: 2.057 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.66CysAla: 0.66 ± 0.023	0.204CysCys: 0.204 ± 0.015	0.716CysAsp: 0.716 ± 0.024	0.785CysGlu: 0.785 ± 0.024	0.578CysPhe: 0.578 ± 0.019	1.142CysGly: 1.142 ± 0.036	0.239CysHis: 0.239 ± 0.013	1.21CysIle: 1.21 ± 0.031	0.928CysLys: 0.928 ± 0.03	0.99CysLeu: 0.99 ± 0.032	0.291CysMet: 0.291 ± 0.016	0.745CysAsn: 0.745 ± 0.02	0.475CysPro: 0.475 ± 0.019	0.211CysGln: 0.211 ± 0.013	0.382CysArg: 0.382 ± 0.018	0.852CysSer: 0.852 ± 0.025	0.659CysThr: 0.659 ± 0.025	0.723CysVal: 0.723 ± 0.026	0.086CysTrp: 0.086 ± 0.009	0.476CysTyr: 0.476 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	2.843AspAla: 2.843 ± 0.053	0.634AspCys: 0.634 ± 0.024	2.744AspAsp: 2.744 ± 0.047	4.141AspGlu: 4.141 ± 0.063	2.636AspPhe: 2.636 ± 0.055	3.318AspGly: 3.318 ± 0.056	0.626AspHis: 0.626 ± 0.02	6.167AspIle: 6.167 ± 0.082	5.201AspLys: 5.201 ± 0.071	4.749AspLeu: 4.749 ± 0.067	1.516AspMet: 1.516 ± 0.033	3.404AspAsn: 3.404 ± 0.05	1.306AspPro: 1.306 ± 0.029	0.904AspGln: 0.904 ± 0.025	1.653AspArg: 1.653 ± 0.041	3.071AspSer: 3.071 ± 0.053	2.722AspThr: 2.722 ± 0.047	3.678AspVal: 3.678 ± 0.053	0.364AspTrp: 0.364 ± 0.016	2.384AspTyr: 2.384 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	3.652GluAla: 3.652 ± 0.061	0.687GluCys: 0.687 ± 0.024	3.63GluAsp: 3.63 ± 0.06	5.12GluGlu: 5.12 ± 0.087	2.965GluPhe: 2.965 ± 0.052	3.68GluGly: 3.68 ± 0.058	0.888GluHis: 0.888 ± 0.028	6.821GluIle: 6.821 ± 0.081	6.732GluLys: 6.732 ± 0.094	6.247GluLeu: 6.247 ± 0.074	1.85GluMet: 1.85 ± 0.042	4.673GluAsn: 4.673 ± 0.061	1.325GluPro: 1.325 ± 0.035	1.632GluGln: 1.632 ± 0.039	2.165GluArg: 2.165 ± 0.047	3.526GluSer: 3.526 ± 0.05	2.893GluThr: 2.893 ± 0.044	4.503GluVal: 4.503 ± 0.067	0.433GluTrp: 0.433 ± 0.022	2.777GluTyr: 2.777 ± 0.053	0.0GluXaa: 0.0 ± 0.0
Phe	2.384PheAla: 2.384 ± 0.047	0.591PheCys: 0.591 ± 0.023	2.499PheAsp: 2.499 ± 0.048	2.624PheGlu: 2.624 ± 0.049	1.82PhePhe: 1.82 ± 0.042	2.893PheGly: 2.893 ± 0.044	0.591PheHis: 0.591 ± 0.023	4.723PheIle: 4.723 ± 0.07	3.904PheLys: 3.904 ± 0.056	3.839PheLeu: 3.839 ± 0.062	1.233PheMet: 1.233 ± 0.028	2.86PheAsn: 2.86 ± 0.043	1.154PhePro: 1.154 ± 0.032	0.963PheGln: 0.963 ± 0.027	1.19PheArg: 1.19 ± 0.029	3.166PheSer: 3.166 ± 0.057	2.409PheThr: 2.409 ± 0.04	2.788PheVal: 2.788 ± 0.047	0.328PheTrp: 0.328 ± 0.016	1.803PheTyr: 1.803 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	4.218GlyAla: 4.218 ± 0.076	0.954GlyCys: 0.954 ± 0.032	3.169GlyAsp: 3.169 ± 0.054	4.042GlyGlu: 4.042 ± 0.063	3.153GlyPhe: 3.153 ± 0.053	4.422GlyGly: 4.422 ± 0.073	1.006GlyHis: 1.006 ± 0.033	7.026GlyIle: 7.026 ± 0.082	5.603GlyLys: 5.603 ± 0.074	5.555GlyLeu: 5.555 ± 0.062	1.87GlyMet: 1.87 ± 0.035	3.511GlyAsn: 3.511 ± 0.066	1.287GlyPro: 1.287 ± 0.046	1.484GlyGln: 1.484 ± 0.04	2.097GlyArg: 2.097 ± 0.043	3.898GlySer: 3.898 ± 0.061	3.731GlyThr: 3.731 ± 0.06	4.625GlyVal: 4.625 ± 0.07	0.484GlyTrp: 0.484 ± 0.019	2.912GlyTyr: 2.912 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	0.713HisAla: 0.713 ± 0.024	0.229HisCys: 0.229 ± 0.015	0.708HisAsp: 0.708 ± 0.025	0.858HisGlu: 0.858 ± 0.027	0.647HisPhe: 0.647 ± 0.024	1.013HisGly: 1.013 ± 0.027	0.275HisHis: 0.275 ± 0.015	1.342HisIle: 1.342 ± 0.031	1.078HisLys: 1.078 ± 0.029	1.197HisLeu: 1.197 ± 0.03	0.412HisMet: 0.412 ± 0.02	0.835HisAsn: 0.835 ± 0.023	0.607HisPro: 0.607 ± 0.022	0.329HisGln: 0.329 ± 0.015	0.489HisArg: 0.489 ± 0.021	0.907HisSer: 0.907 ± 0.027	0.735HisThr: 0.735 ± 0.023	0.812HisVal: 0.812 ± 0.023	0.123HisTrp: 0.123 ± 0.01	0.565HisTyr: 0.565 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.058IleAla: 6.058 ± 0.076	1.365IleCys: 1.365 ± 0.035	5.771IleAsp: 5.771 ± 0.076	6.674IleGlu: 6.674 ± 0.089	4.366IlePhe: 4.366 ± 0.069	6.533IleGly: 6.533 ± 0.077	1.276IleHis: 1.276 ± 0.033	10.271IleIle: 10.271 ± 0.126	9.179IleLys: 9.179 ± 0.081	9.203IleLeu: 9.203 ± 0.107	2.63IleMet: 2.63 ± 0.047	6.41IleAsn: 6.41 ± 0.073	3.301IlePro: 3.301 ± 0.054	2.29IleGln: 2.29 ± 0.042	3.081IleArg: 3.081 ± 0.058	7.196IleSer: 7.196 ± 0.084	5.596IleThr: 5.596 ± 0.074	6.437IleVal: 6.437 ± 0.077	0.563IleTrp: 0.563 ± 0.025	3.596IleTyr: 3.596 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	4.854LysAla: 4.854 ± 0.064	0.907LysCys: 0.907 ± 0.027	5.585LysAsp: 5.585 ± 0.076	7.363LysGlu: 7.363 ± 0.078	3.234LysPhe: 3.234 ± 0.054	5.256LysGly: 5.256 ± 0.063	1.227LysHis: 1.227 ± 0.033	8.651LysIle: 8.651 ± 0.085	8.011LysLys: 8.011 ± 0.078	7.895LysLeu: 7.895 ± 0.077	2.616LysMet: 2.616 ± 0.046	6.165LysAsn: 6.165 ± 0.074	2.164LysPro: 2.164 ± 0.044	2.554LysGln: 2.554 ± 0.044	2.894LysArg: 2.894 ± 0.045	5.394LysSer: 5.394 ± 0.069	4.672LysThr: 4.672 ± 0.064	5.948LysVal: 5.948 ± 0.069	0.651LysTrp: 0.651 ± 0.024	4.091LysTyr: 4.091 ± 0.06	0.0LysXaa: 0.0 ± 0.0
Leu	5.165LeuAla: 5.165 ± 0.069	1.167LeuCys: 1.167 ± 0.032	4.931LeuAsp: 4.931 ± 0.076	5.693LeuGlu: 5.693 ± 0.089	3.802LeuPhe: 3.802 ± 0.062	5.922LeuGly: 5.922 ± 0.068	1.161LeuHis: 1.161 ± 0.032	8.556LeuIle: 8.556 ± 0.093	8.567LeuLys: 8.567 ± 0.081	8.224LeuLeu: 8.224 ± 0.104	2.448LeuMet: 2.448 ± 0.04	5.622LeuAsn: 5.622 ± 0.073	2.852LeuPro: 2.852 ± 0.048	2.234LeuGln: 2.234 ± 0.041	2.956LeuArg: 2.956 ± 0.056	6.742LeuSer: 6.742 ± 0.077	4.852LeuThr: 4.852 ± 0.061	5.596LeuVal: 5.596 ± 0.078	0.593LeuTrp: 0.593 ± 0.024	3.225LeuTyr: 3.225 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	1.826MetAla: 1.826 ± 0.038	0.281MetCys: 0.281 ± 0.017	1.681MetAsp: 1.681 ± 0.038	1.833MetGlu: 1.833 ± 0.038	1.128MetPhe: 1.128 ± 0.031	1.877MetGly: 1.877 ± 0.037	0.344MetHis: 0.344 ± 0.016	2.425MetIle: 2.425 ± 0.046	2.619MetLys: 2.619 ± 0.047	2.442MetLeu: 2.442 ± 0.039	0.716MetMet: 0.716 ± 0.029	1.73MetAsn: 1.73 ± 0.038	0.97MetPro: 0.97 ± 0.032	0.741MetGln: 0.741 ± 0.024	0.851MetArg: 0.851 ± 0.026	1.788MetSer: 1.788 ± 0.041	1.27MetThr: 1.27 ± 0.03	1.804MetVal: 1.804 ± 0.039	0.18MetTrp: 0.18 ± 0.012	0.923MetTyr: 0.923 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	3.056AsnAla: 3.056 ± 0.051	0.766AsnCys: 0.766 ± 0.024	3.163AsnAsp: 3.163 ± 0.053	4.121AsnGlu: 4.121 ± 0.071	2.543AsnPhe: 2.543 ± 0.045	3.78AsnGly: 3.78 ± 0.064	0.808AsnHis: 0.808 ± 0.028	7.084AsnIle: 7.084 ± 0.086	6.13AsnLys: 6.13 ± 0.075	5.456AsnLeu: 5.456 ± 0.069	1.724AsnMet: 1.724 ± 0.036	4.347AsnAsn: 4.347 ± 0.084	2.016AsnPro: 2.016 ± 0.039	1.367AsnGln: 1.367 ± 0.035	1.781AsnArg: 1.781 ± 0.041	4.012AsnSer: 4.012 ± 0.064	2.95AsnThr: 2.95 ± 0.052	3.891AsnVal: 3.891 ± 0.053	0.476AsnTrp: 0.476 ± 0.02	2.609AsnTyr: 2.609 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	1.432ProAla: 1.432 ± 0.036	0.338ProCys: 0.338 ± 0.015	1.413ProAsp: 1.413 ± 0.036	1.974ProGlu: 1.974 ± 0.043	1.371ProPhe: 1.371 ± 0.033	1.714ProGly: 1.714 ± 0.039	0.477ProHis: 0.477 ± 0.02	2.867ProIle: 2.867 ± 0.049	2.215ProLys: 2.215 ± 0.049	2.595ProLeu: 2.595 ± 0.045	0.769ProMet: 0.769 ± 0.027	1.5ProAsn: 1.5 ± 0.034	0.564ProPro: 0.564 ± 0.021	0.792ProGln: 0.792 ± 0.022	0.83ProArg: 0.83 ± 0.026	1.826ProSer: 1.826 ± 0.037	1.695ProThr: 1.695 ± 0.045	1.999ProVal: 1.999 ± 0.046	0.248ProTrp: 0.248 ± 0.014	1.237ProTyr: 1.237 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	1.388GlnAla: 1.388 ± 0.034	0.306GlnCys: 0.306 ± 0.016	1.198GlnAsp: 1.198 ± 0.028	1.553GlnGlu: 1.553 ± 0.035	1.051GlnPhe: 1.051 ± 0.031	1.474GlnGly: 1.474 ± 0.035	0.366GlnHis: 0.366 ± 0.02	2.366GlnIle: 2.366 ± 0.047	2.235GlnLys: 2.235 ± 0.04	2.149GlnLeu: 2.149 ± 0.041	0.712GlnMet: 0.712 ± 0.025	1.588GlnAsn: 1.588 ± 0.035	0.573GlnPro: 0.573 ± 0.019	0.772GlnGln: 0.772 ± 0.025	0.954GlnArg: 0.954 ± 0.033	1.551GlnSer: 1.551 ± 0.04	1.258GlnThr: 1.258 ± 0.033	1.625GlnVal: 1.625 ± 0.041	0.227GlnTrp: 0.227 ± 0.014	1.085GlnTyr: 1.085 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	1.815ArgAla: 1.815 ± 0.044	0.369ArgCys: 0.369 ± 0.018	1.742ArgAsp: 1.742 ± 0.039	2.465ArgGlu: 2.465 ± 0.044	1.362ArgPhe: 1.362 ± 0.031	2.003ArgGly: 2.003 ± 0.044	0.453ArgHis: 0.453 ± 0.022	3.003ArgIle: 3.003 ± 0.054	2.885ArgLys: 2.885 ± 0.053	2.809ArgLeu: 2.809 ± 0.055	0.926ArgMet: 0.926 ± 0.024	1.882ArgAsn: 1.882 ± 0.039	0.844ArgPro: 0.844 ± 0.023	0.886ArgGln: 0.886 ± 0.028	1.328ArgArg: 1.328 ± 0.035	1.655ArgSer: 1.655 ± 0.038	1.556ArgThr: 1.556 ± 0.036	2.126ArgVal: 2.126 ± 0.045	0.249ArgTrp: 0.249 ± 0.011	1.261ArgTyr: 1.261 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	3.384SerAla: 3.384 ± 0.061	0.749SerCys: 0.749 ± 0.023	3.372SerAsp: 3.372 ± 0.056	3.917SerGlu: 3.917 ± 0.063	3.03SerPhe: 3.03 ± 0.047	4.643SerGly: 4.643 ± 0.064	0.897SerHis: 0.897 ± 0.027	6.762SerIle: 6.762 ± 0.084	5.982SerLys: 5.982 ± 0.065	6.074SerLeu: 6.074 ± 0.074	1.756SerMet: 1.756 ± 0.036	3.965SerAsn: 3.965 ± 0.058	1.71SerPro: 1.71 ± 0.042	1.664SerGln: 1.664 ± 0.035	2.057SerArg: 2.057 ± 0.043	4.598SerSer: 4.598 ± 0.075	3.571SerThr: 3.571 ± 0.062	4.098SerVal: 4.098 ± 0.055	0.44SerTrp: 0.44 ± 0.018	2.593SerTyr: 2.593 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	3.167ThrAla: 3.167 ± 0.055	0.567ThrCys: 0.567 ± 0.02	2.576ThrAsp: 2.576 ± 0.044	2.853ThrGlu: 2.853 ± 0.048	2.373ThrPhe: 2.373 ± 0.046	3.927ThrGly: 3.927 ± 0.089	0.821ThrHis: 0.821 ± 0.028	5.263ThrIle: 5.263 ± 0.077	4.331ThrLys: 4.331 ± 0.063	5.049ThrLeu: 5.049 ± 0.065	1.341ThrMet: 1.341 ± 0.035	3.051ThrAsn: 3.051 ± 0.06	1.905ThrPro: 1.905 ± 0.044	1.441ThrGln: 1.441 ± 0.042	1.577ThrArg: 1.577 ± 0.036	3.642ThrSer: 3.642 ± 0.056	3.151ThrThr: 3.151 ± 0.064	3.556ThrVal: 3.556 ± 0.06	0.415ThrTrp: 0.415 ± 0.017	2.137ThrTyr: 2.137 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	4.185ValAla: 4.185 ± 0.069	0.89ValCys: 0.89 ± 0.028	3.761ValAsp: 3.761 ± 0.055	4.14ValGlu: 4.14 ± 0.061	2.916ValPhe: 2.916 ± 0.048	4.348ValGly: 4.348 ± 0.06	0.917ValHis: 0.917 ± 0.024	6.658ValIle: 6.658 ± 0.075	5.416ValLys: 5.416 ± 0.072	5.974ValLeu: 5.974 ± 0.076	1.771ValMet: 1.771 ± 0.04	3.753ValAsn: 3.753 ± 0.051	2.037ValPro: 2.037 ± 0.039	1.536ValGln: 1.536 ± 0.037	1.865ValArg: 1.865 ± 0.032	4.449ValSer: 4.449 ± 0.057	3.715ValThr: 3.715 ± 0.054	4.816ValVal: 4.816 ± 0.071	0.424ValTrp: 0.424 ± 0.015	2.452ValTyr: 2.452 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	0.387TrpAla: 0.387 ± 0.017	0.105TrpCys: 0.105 ± 0.009	0.407TrpAsp: 0.407 ± 0.019	0.403TrpGlu: 0.403 ± 0.016	0.35TrpPhe: 0.35 ± 0.018	0.512TrpGly: 0.512 ± 0.021	0.143TrpHis: 0.143 ± 0.01	0.677TrpIle: 0.677 ± 0.025	0.558TrpLys: 0.558 ± 0.02	0.591TrpLeu: 0.591 ± 0.027	0.185TrpMet: 0.185 ± 0.012	0.479TrpAsn: 0.479 ± 0.02	0.151TrpPro: 0.151 ± 0.009	0.203TrpGln: 0.203 ± 0.01	0.252TrpArg: 0.252 ± 0.015	0.489TrpSer: 0.489 ± 0.021	0.35TrpThr: 0.35 ± 0.018	0.463TrpVal: 0.463 ± 0.018	0.091TrpTrp: 0.091 ± 0.008	0.265TrpTyr: 0.265 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.056TyrAla: 2.056 ± 0.038	0.532TyrCys: 0.532 ± 0.022	2.362TyrAsp: 2.362 ± 0.05	2.564TyrGlu: 2.564 ± 0.051	1.934TyrPhe: 1.934 ± 0.038	2.63TyrGly: 2.63 ± 0.044	0.555TyrHis: 0.555 ± 0.02	3.927TyrIle: 3.927 ± 0.065	3.711TyrLys: 3.711 ± 0.062	3.603TyrLeu: 3.603 ± 0.05	1.005TyrMet: 1.005 ± 0.024	2.75TyrAsn: 2.75 ± 0.047	1.218TyrPro: 1.218 ± 0.029	0.773TyrGln: 0.773 ± 0.024	1.319TyrArg: 1.319 ± 0.035	2.761TyrSer: 2.761 ± 0.044	2.087TyrThr: 2.087 ± 0.042	2.365TyrVal: 2.365 ± 0.046	0.295TyrTrp: 0.295 ± 0.017	1.758TyrTyr: 1.758 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4203 proteins (1303679 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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