Thermanaeromonas toyohensis ToBE

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacteraceae; Thermanaeromonas; Thermanaeromonas toyohensis

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W1VV16|A0A1W1VV16_9THEO 4Fe-4S dicluster domain-containing protein OS=Thermanaeromonas toyohensis ToBE OX=698762 GN=SAMN00808754_1788 PE=4 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84VYY4 pKa = 10.96VDD6 pKa = 3.89QDD8 pKa = 3.48LCTGCGACIDD18 pKa = 4.34LCPEE22 pKa = 3.7VFDD25 pKa = 4.6WDD27 pKa = 4.22DD28 pKa = 3.63EE29 pKa = 4.65GSSRR33 pKa = 11.84VIVDD37 pKa = 3.88EE38 pKa = 4.25VPEE41 pKa = 4.33DD42 pKa = 4.13LEE44 pKa = 4.69DD45 pKa = 3.97CVRR48 pKa = 11.84EE49 pKa = 4.21AVDD52 pKa = 3.64TCPSGAIRR60 pKa = 11.84EE61 pKa = 4.36VV62 pKa = 3.16

Molecular weight: 6.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W1V5C2|A0A1W1V5C2_9THEO Flavin-dependent thymidylate synthase OS=Thermanaeromonas toyohensis ToBE OX=698762 GN=thyX PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.48QPKK8 pKa = 8.19RR9 pKa = 11.84RR10 pKa = 11.84HH11 pKa = 5.87RR12 pKa = 11.84KK13 pKa = 8.51RR14 pKa = 11.84VHH16 pKa = 6.1GFLKK20 pKa = 10.56RR21 pKa = 11.84MSTRR25 pKa = 11.84AGRR28 pKa = 11.84EE29 pKa = 3.28ILRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.87GRR39 pKa = 11.84KK40 pKa = 9.02RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3229

0

3229

972887

30

1548

301.3

33.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.255 ± 0.044

1.18 ± 0.02

4.002 ± 0.029

7.712 ± 0.05

3.629 ± 0.03

8.291 ± 0.044

1.705 ± 0.018

6.146 ± 0.037

5.172 ± 0.032

11.607 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.029 ± 0.019

2.767 ± 0.026

4.953 ± 0.029

3.333 ± 0.03

6.543 ± 0.041

4.767 ± 0.032

4.661 ± 0.032

7.868 ± 0.039

1.288 ± 0.019

3.094 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski