Escherichia phage P AB-2017

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; unclassified Kagunavirus

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q1PV71|A0A1Q1PV71_9CAUD Putative holin-like class II OS=Escherichia phage P AB-2017 OX=1933115 GN=P_41 PE=4 SV=1
MM1 pKa = 7.56PLIVEE6 pKa = 5.08DD7 pKa = 4.46GSIVPDD13 pKa = 3.62ADD15 pKa = 4.01SYY17 pKa = 12.14VSLADD22 pKa = 3.76ARR24 pKa = 11.84ALAANYY30 pKa = 10.58GLGLPADD37 pKa = 3.84DD38 pKa = 4.13TAAEE42 pKa = 4.04VALRR46 pKa = 11.84NGATYY51 pKa = 10.78VGLAEE56 pKa = 4.18PQMCGSRR63 pKa = 11.84VSAEE67 pKa = 3.6QSLAYY72 pKa = 9.45PRR74 pKa = 11.84TGVTLNGFSVANNTIPKK91 pKa = 9.62QVILAQVIAAATYY104 pKa = 9.32GAGTEE109 pKa = 4.04VRR111 pKa = 11.84ANSDD115 pKa = 2.89GRR117 pKa = 11.84SVQTEE122 pKa = 4.03RR123 pKa = 11.84VEE125 pKa = 4.36GAVTVTYY132 pKa = 10.03FNNGSSGATTAITAADD148 pKa = 3.78DD149 pKa = 3.82ALRR152 pKa = 11.84PLLCGGLNNGFSFNVYY168 pKa = 10.14RR169 pKa = 11.84GG170 pKa = 3.26

Molecular weight: 17.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q1PV70|A0A1Q1PV70_9CAUD Uncharacterized protein OS=Escherichia phage P AB-2017 OX=1933115 GN=P_20 PE=4 SV=1
MM1 pKa = 7.55SEE3 pKa = 4.23QGPVSQPLRR12 pKa = 11.84VGRR15 pKa = 11.84KK16 pKa = 8.08VSHH19 pKa = 6.57APFPTRR25 pKa = 11.84EE26 pKa = 3.93EE27 pKa = 3.9LMKK30 pKa = 10.72RR31 pKa = 11.84NSFPGPDD38 pKa = 3.12KK39 pKa = 11.2NKK41 pKa = 9.97YY42 pKa = 8.91LNRR45 pKa = 11.84MWGARR50 pKa = 11.84KK51 pKa = 9.63NDD53 pKa = 3.29

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12555

30

984

202.5

22.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.996 ± 0.474

1.051 ± 0.135

6.197 ± 0.247

6.523 ± 0.464

3.775 ± 0.135

7.662 ± 0.23

1.665 ± 0.172

5.161 ± 0.136

5.902 ± 0.357

7.83 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.533 ± 0.161

4.182 ± 0.247

3.919 ± 0.235

3.751 ± 0.318

5.305 ± 0.271

6.173 ± 0.335

6.468 ± 0.339

7.017 ± 0.327

1.442 ± 0.146

3.449 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski