Deinococcus aerius
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4445 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I9CYD8|A0A2I9CYD8_9DEIO Cytochrome P450 cyp OS=Deinococcus aerius OX=200253 GN=DAERI_120156 PE=3 SV=1
MM1 pKa = 6.5 QTPSEE6 pKa = 4.12 LKK8 pKa = 10.12 YY9 pKa = 11.03 APTHH13 pKa = 5.3 EE14 pKa = 4.2 WLAEE18 pKa = 4.43 DD19 pKa = 3.99 GTVGISDD26 pKa = 4.46 FAQDD30 pKa = 3.29 QLGDD34 pKa = 3.68 VVYY37 pKa = 11.21 VEE39 pKa = 4.52 LPEE42 pKa = 4.03 VGRR45 pKa = 11.84 VVTAGEE51 pKa = 4.09 TVAVVEE57 pKa = 4.56 SVKK60 pKa = 10.0 TASDD64 pKa = 3.45 IYY66 pKa = 11.02 APASGTITAVNEE78 pKa = 4.06 QLSGSPEE85 pKa = 4.05 LVNSGPYY92 pKa = 9.66 GDD94 pKa = 4.02 GWLFRR99 pKa = 11.84 LDD101 pKa = 3.5 VTEE104 pKa = 4.23 EE105 pKa = 4.03 SGDD108 pKa = 3.76 LMDD111 pKa = 5.43 AEE113 pKa = 5.41 AYY115 pKa = 10.09 SAANSS120 pKa = 3.56
Molecular weight: 12.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.719
IPC_protein 3.643
Toseland 3.465
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.554
Rodwell 3.49
Grimsley 3.376
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 3.91
Thurlkill 3.516
EMBOSS 3.567
Sillero 3.77
Patrickios 1.812
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.721
Protein with the highest isoelectric point:
>tr|A0A2I9CZ82|A0A2I9CZ82_9DEIO NUDIX hydrolase OS=Deinococcus aerius OX=200253 GN=DAERI_150041 PE=3 SV=1
MM1 pKa = 7.29 TVPLPAALVASLNDD15 pKa = 3.56 SRR17 pKa = 11.84 PCQPEE22 pKa = 3.93 RR23 pKa = 11.84 GCGLRR28 pKa = 11.84 AFDD31 pKa = 4.27 AVHH34 pKa = 6.45 FAAAQAVSPLGVQLMTFDD52 pKa = 3.82 LKK54 pKa = 11.05 LRR56 pKa = 11.84 AVAEE60 pKa = 4.02 QAMPGQVWQSEE71 pKa = 3.99 RR72 pKa = 11.84 RR73 pKa = 11.84 ILFLCPLRR81 pKa = 11.84 LPFRR85 pKa = 11.84 APCVAGWQPEE95 pKa = 4.42 TPRR98 pKa = 11.84 LAGPGSAQPNFTRR111 pKa = 11.84 DD112 pKa = 2.61 AWGEE116 pKa = 3.79 FIKK119 pKa = 10.59 QKK121 pKa = 9.1 RR122 pKa = 11.84 HH123 pKa = 3.75 SRR125 pKa = 11.84 AVLRR129 pKa = 11.84 HH130 pKa = 3.66 QRR132 pKa = 11.84 GRR134 pKa = 11.84 YY135 pKa = 6.74 IRR137 pKa = 11.84 RR138 pKa = 11.84 RR139 pKa = 11.84 LRR141 pKa = 11.84 YY142 pKa = 9.04 AYY144 pKa = 10.17 AGEE147 pKa = 4.09 WRR149 pKa = 11.84 PKK151 pKa = 9.68 PVWAWWYY158 pKa = 9.45 RR159 pKa = 11.84 VPGQASKK166 pKa = 11.53 NNTVCSCGLCRR177 pKa = 11.84 AEE179 pKa = 5.1 KK180 pKa = 10.78 YY181 pKa = 9.79 RR182 pKa = 11.84 DD183 pKa = 3.41 KK184 pKa = 10.95 PRR186 pKa = 11.84 QRR188 pKa = 11.84 WWDD191 pKa = 3.57 EE192 pKa = 3.69 EE193 pKa = 4.18
Molecular weight: 22.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.56
IPC_protein 10.438
Toseland 10.511
ProMoST 10.277
Dawson 10.643
Bjellqvist 10.409
Wikipedia 10.877
Rodwell 10.76
Grimsley 10.701
Solomon 10.76
Lehninger 10.73
Nozaki 10.57
DTASelect 10.379
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.599
Patrickios 10.452
IPC_peptide 10.76
IPC2_peptide 9.765
IPC2.peptide.svr19 8.501
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4445
0
4445
1341196
25
3545
301.7
32.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.326 ± 0.057
0.578 ± 0.01
4.981 ± 0.029
5.836 ± 0.039
3.161 ± 0.022
9.633 ± 0.038
2.16 ± 0.022
3.138 ± 0.028
2.123 ± 0.031
11.831 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.719 ± 0.016
2.359 ± 0.028
6.179 ± 0.032
3.316 ± 0.025
8.175 ± 0.042
4.604 ± 0.025
5.968 ± 0.034
8.091 ± 0.036
1.452 ± 0.017
2.368 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here