Proteome-pI 2.0 - Proteome Isoelectric Point Database

Pythium polare RNA virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 5.26

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A2Z6C4V5|A0A2Z6C4V5_9VIRU Putative coat protein OS=Pythium polare RNA virus 2 OX=2137354 GN=CP PE=4 SV=1

MM1 pKa = 7.06SQNLFYY7 pKa = 10.72DD8 pKa = 3.21SHH10 pKa = 8.0IPEE13 pKa = 5.02HH14 pKa = 5.91STGLTLAEE22 pKa = 4.58FPALVKK28 pKa = 9.98TDD30 pKa = 3.7YY31 pKa = 11.75ANLATYY37 pKa = 10.66SRR39 pKa = 11.84DD40 pKa = 3.31NLRR43 pKa = 11.84QQLSLQLPFLPPDD56 pKa = 3.14SRR58 pKa = 11.84GRR60 pKa = 11.84IAVGRR65 pKa = 11.84VDD67 pKa = 4.13LAYY70 pKa = 10.09EE71 pKa = 4.74IIRR74 pKa = 11.84HH75 pKa = 5.73DD76 pKa = 3.97PEE78 pKa = 5.93NGQSSTVVGRR88 pKa = 11.84AHH90 pKa = 6.36VPSQAEE96 pKa = 3.89IGFFTAPSHH105 pKa = 6.6DD106 pKa = 3.62PMRR109 pKa = 11.84IIMAPVSAPLAADD122 pKa = 3.53VVDD125 pKa = 4.68KK126 pKa = 11.43NVATATTSIKK136 pKa = 10.84ALFPNQQPNEE146 pKa = 4.26YY147 pKa = 9.24IGPNARR153 pKa = 11.84ARR155 pKa = 11.84IRR157 pKa = 11.84SLVAKK162 pKa = 9.32PYY164 pKa = 9.82NPEE167 pKa = 3.68RR168 pKa = 11.84YY169 pKa = 7.79VWRR172 pKa = 11.84LASLYY177 pKa = 10.38LASAWAKK184 pKa = 10.38ANKK187 pKa = 7.5EE188 pKa = 4.37TLTSSQLNLPTITWLNSVTAYY209 pKa = 10.6GAVLDD214 pKa = 3.94NWSGSEE220 pKa = 4.0NPVCVAYY227 pKa = 10.09EE228 pKa = 4.17GFPSEE233 pKa = 4.22VTPLLGVLQLALSGDD248 pKa = 3.42PAFRR252 pKa = 11.84VTGNLPLPSVATVWPTIALPHH273 pKa = 6.33VYY275 pKa = 10.44YY276 pKa = 10.28IGPQVTTTIGVGDD289 pKa = 4.05ITPEE293 pKa = 4.22LVWSAASLWCGQHH306 pKa = 6.53GCMQLLNDD314 pKa = 4.61YY315 pKa = 10.32IATLSTLWCSTHH327 pKa = 6.93KK328 pKa = 10.7DD329 pKa = 3.64LSPVYY334 pKa = 7.46QTKK337 pKa = 10.25RR338 pKa = 11.84FGMALPVSDD347 pKa = 5.13LSSTILMPIGVSYY360 pKa = 9.56VKK362 pKa = 10.25WRR364 pKa = 11.84DD365 pKa = 2.93EE366 pKa = 4.01GMLVDD371 pKa = 4.73PPRR374 pKa = 11.84EE375 pKa = 3.62EE376 pKa = 5.31DD377 pKa = 3.36LFLRR381 pKa = 11.84GALLGVLMGLGVRR394 pKa = 11.84TWAYY398 pKa = 10.65RR399 pKa = 11.84SGLPYY404 pKa = 10.67VGLLSGADD412 pKa = 3.56TEE414 pKa = 4.21RR415 pKa = 11.84DD416 pKa = 3.15RR417 pKa = 11.84VFRR420 pKa = 11.84EE421 pKa = 4.05FKK423 pKa = 10.4LASHH427 pKa = 6.8ATGSMFNAQRR437 pKa = 11.84VLQNLGCTGTLGAILLSLTPAFAHH461 pKa = 6.51RR462 pKa = 11.84RR463 pKa = 11.84DD464 pKa = 3.69LASWWSLQDD473 pKa = 5.3DD474 pKa = 4.81AYY476 pKa = 11.04QWEE479 pKa = 4.64EE480 pKa = 3.83VANVCSSIPRR490 pKa = 11.84CCALAGLIQPLRR502 pKa = 11.84ATSMPMVNVWYY513 pKa = 9.97APDD516 pKa = 3.94VISSARR522 pKa = 11.84CVEE525 pKa = 4.1EE526 pKa = 4.46AFQGLLYY533 pKa = 10.95SGGLVAGWVIKK544 pKa = 9.73DD545 pKa = 3.42ARR547 pKa = 11.84GGTTATYY554 pKa = 9.25PVTIIKK560 pKa = 9.93SYY562 pKa = 10.8RR563 pKa = 11.84GAASDD568 pKa = 3.49WQFAAPYY575 pKa = 8.57TKK577 pKa = 10.29DD578 pKa = 3.49YY579 pKa = 11.03VHH581 pKa = 8.31AEE583 pKa = 4.09MVFKK587 pKa = 9.87LTSVGATLLATTGPIGMSRR606 pKa = 11.84AKK608 pKa = 9.67WYY610 pKa = 10.48LSRR613 pKa = 11.84PTVPSDD619 pKa = 3.47YY620 pKa = 11.02QLDD623 pKa = 4.27ADD625 pKa = 4.48WKK627 pKa = 9.71AGPNAGGGPSGPPPPPPSQPPPEE650 pKa = 4.79PPGTPPDD657 pKa = 3.68YY658 pKa = 10.1STRR661 pKa = 11.84ASDD664 pKa = 4.26PPRR667 pKa = 11.84DD668 pKa = 3.89PNAPTDD674 pKa = 4.04DD675 pKa = 4.05VPPALTTTMGPDD687 pKa = 3.06TTTAALPHH695 pKa = 5.68SLKK698 pKa = 10.73LRR700 pKa = 11.84VEE702 pKa = 4.21TARR705 pKa = 11.84AALHH709 pKa = 7.0DD710 pKa = 5.01AGQSTDD716 pKa = 2.9WLEE719 pKa = 4.0SLTLGLQRR727 pKa = 11.84RR728 pKa = 11.84TTLSTPWEE736 pKa = 4.2DD737 pKa = 3.22RR738 pKa = 11.84DD739 pKa = 3.6RR740 pKa = 11.84EE741 pKa = 4.25GRR743 pKa = 11.84MRR745 pKa = 11.84AALDD749 pKa = 3.29GMQITDD755 pKa = 4.7PIEE758 pKa = 3.79MLKK761 pKa = 10.6AVPNGSRR768 pKa = 11.84ANVATCLAAAYY779 pKa = 8.56KK780 pKa = 10.51AAVPCAHH787 pKa = 6.76SLSAAHH793 pKa = 5.87VWAVEE798 pKa = 3.93AGRR801 pKa = 11.84MSSRR805 pKa = 11.84ARR807 pKa = 11.84ALRR810 pKa = 11.84ACSAMTRR817 pKa = 11.84VEE819 pKa = 4.1LEE821 pKa = 4.46DD822 pKa = 3.42YY823 pKa = 7.11TTRR826 pKa = 11.84HH827 pKa = 5.08AVKK830 pKa = 10.37NAALSVDD837 pKa = 4.01DD838 pKa = 4.83GSIAKK843 pKa = 10.35ALGRR847 pKa = 11.84GIAAKK852 pKa = 10.1EE853 pKa = 4.06LIGSAGKK860 pKa = 10.51RR861 pKa = 11.84SGDD864 pKa = 2.98KK865 pKa = 8.53WTADD869 pKa = 3.29PVIEE873 pKa = 4.65RR874 pKa = 11.84KK875 pKa = 9.34LAAPDD880 pKa = 3.92APSDD884 pKa = 4.45AISSDD889 pKa = 3.65TEE891 pKa = 4.46DD892 pKa = 3.81MIKK895 pKa = 9.71TSYY898 pKa = 10.91VDD900 pKa = 3.51EE901 pKa = 4.92QIDD904 pKa = 3.99DD905 pKa = 3.86EE906 pKa = 4.52MVIAILGYY914 pKa = 9.75APAWLRR920 pKa = 11.84QSSEE924 pKa = 4.09VPGTDD929 pKa = 2.86GDD931 pKa = 4.25EE932 pKa = 4.26EE933 pKa = 4.41QKK935 pKa = 11.06EE936 pKa = 4.34SDD938 pKa = 4.34PPDD941 pKa = 4.07PMDD944 pKa = 4.05LATTIGSVTARR955 pKa = 11.84VRR957 pKa = 11.84GRR959 pKa = 11.84SLEE962 pKa = 4.34DD963 pKa = 3.32MAKK966 pKa = 10.11HH967 pKa = 6.0APADD971 pKa = 3.47QRR973 pKa = 11.84LDD975 pKa = 3.44RR976 pKa = 11.84RR977 pKa = 11.84PVKK980 pKa = 10.65VAVSWDD986 pKa = 3.61TLNTVAQARR995 pKa = 11.84ARR997 pKa = 11.84SKK999 pKa = 10.82IEE1001 pKa = 4.15GGDD1004 pKa = 3.76VEE1006 pKa = 4.25VDD1008 pKa = 3.84GYY1010 pKa = 10.66LVRR1013 pKa = 11.84QDD1015 pKa = 3.78GSVEE1019 pKa = 4.0QISFNARR1026 pKa = 11.84GEE1028 pKa = 4.14AMHH1031 pKa = 6.87ARR1033 pKa = 11.84VEE1035 pKa = 4.58LITEE1039 pKa = 4.12EE1040 pKa = 4.56LDD1042 pKa = 3.21PHH1044 pKa = 5.8ITVSSVTAAPEE1055 pKa = 3.9ADD1057 pKa = 3.17KK1058 pKa = 11.29HH1059 pKa = 5.54VVTPAGTTVLEE1070 pKa = 4.16EE1071 pKa = 3.98QEE1073 pKa = 4.84AIADD1077 pKa = 3.66IAGSGTQTADD1087 pKa = 3.21FGLHH1091 pKa = 5.6TDD1093 pKa = 4.03ITPMLPPMPPPASSTASPTTTTTSQSHH1120 pKa = 6.32GKK1122 pKa = 8.44QSRR1125 pKa = 11.84TPTPKK1130 pKa = 9.89RR1131 pKa = 11.84QSSNAPPSDD1140 pKa = 3.17AQEE1143 pKa = 3.59IHH1145 pKa = 6.68RR1146 pKa = 11.84LVFKK1150 pKa = 10.67EE1151 pKa = 4.17QDD1153 pKa = 3.36DD1154 pKa = 4.09TT1155 pKa = 4.46

Molecular weight: 124.54 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A2Z6C4V5|A0A2Z6C4V5_9VIRU Putative coat protein OS=Pythium polare RNA virus 2 OX=2137354 GN=CP PE=4 SV=1

MM1 pKa = 7.06SQNLFYY7 pKa = 10.72DD8 pKa = 3.21SHH10 pKa = 8.0IPEE13 pKa = 5.02HH14 pKa = 5.91STGLTLAEE22 pKa = 4.58FPALVKK28 pKa = 9.98TDD30 pKa = 3.7YY31 pKa = 11.75ANLATYY37 pKa = 10.66SRR39 pKa = 11.84DD40 pKa = 3.31NLRR43 pKa = 11.84QQLSLQLPFLPPDD56 pKa = 3.14SRR58 pKa = 11.84GRR60 pKa = 11.84IAVGRR65 pKa = 11.84VDD67 pKa = 4.13LAYY70 pKa = 10.09EE71 pKa = 4.74IIRR74 pKa = 11.84HH75 pKa = 5.73DD76 pKa = 3.97PEE78 pKa = 5.93NGQSSTVVGRR88 pKa = 11.84AHH90 pKa = 6.36VPSQAEE96 pKa = 3.89IGFFTAPSHH105 pKa = 6.6DD106 pKa = 3.62PMRR109 pKa = 11.84IIMAPVSAPLAADD122 pKa = 3.53VVDD125 pKa = 4.68KK126 pKa = 11.43NVATATTSIKK136 pKa = 10.84ALFPNQQPNEE146 pKa = 4.26YY147 pKa = 9.24IGPNARR153 pKa = 11.84ARR155 pKa = 11.84IRR157 pKa = 11.84SLVAKK162 pKa = 9.32PYY164 pKa = 9.82NPEE167 pKa = 3.68RR168 pKa = 11.84YY169 pKa = 7.79VWRR172 pKa = 11.84LASLYY177 pKa = 10.38LASAWAKK184 pKa = 10.38ANKK187 pKa = 7.5EE188 pKa = 4.37TLTSSQLNLPTITWLNSVTAYY209 pKa = 10.6GAVLDD214 pKa = 3.94NWSGSEE220 pKa = 4.0NPVCVAYY227 pKa = 10.09EE228 pKa = 4.17GFPSEE233 pKa = 4.22VTPLLGVLQLALSGDD248 pKa = 3.42PAFRR252 pKa = 11.84VTGNLPLPSVATVWPTIALPHH273 pKa = 6.33VYY275 pKa = 10.44YY276 pKa = 10.28IGPQVTTTIGVGDD289 pKa = 4.05ITPEE293 pKa = 4.22LVWSAASLWCGQHH306 pKa = 6.53GCMQLLNDD314 pKa = 4.61YY315 pKa = 10.32IATLSTLWCSTHH327 pKa = 6.93KK328 pKa = 10.7DD329 pKa = 3.64LSPVYY334 pKa = 7.46QTKK337 pKa = 10.25RR338 pKa = 11.84FGMALPVSDD347 pKa = 5.13LSSTILMPIGVSYY360 pKa = 9.56VKK362 pKa = 10.25WRR364 pKa = 11.84DD365 pKa = 2.93EE366 pKa = 4.01GMLVDD371 pKa = 4.73PPRR374 pKa = 11.84EE375 pKa = 3.62EE376 pKa = 5.31DD377 pKa = 3.36LFLRR381 pKa = 11.84GALLGVLMGLGVRR394 pKa = 11.84TWAYY398 pKa = 10.65RR399 pKa = 11.84SGLPYY404 pKa = 10.67VGLLSGADD412 pKa = 3.56TEE414 pKa = 4.21RR415 pKa = 11.84DD416 pKa = 3.15RR417 pKa = 11.84VFRR420 pKa = 11.84EE421 pKa = 4.05FKK423 pKa = 10.4LASHH427 pKa = 6.8ATGSMFNAQRR437 pKa = 11.84VLQNLGCTGTLGAILLSLTPAFAHH461 pKa = 6.51RR462 pKa = 11.84RR463 pKa = 11.84DD464 pKa = 3.69LASWWSLQDD473 pKa = 5.3DD474 pKa = 4.81AYY476 pKa = 11.04QWEE479 pKa = 4.64EE480 pKa = 3.83VANVCSSIPRR490 pKa = 11.84CCALAGLIQPLRR502 pKa = 11.84ATSMPMVNVWYY513 pKa = 9.97APDD516 pKa = 3.94VISSARR522 pKa = 11.84CVEE525 pKa = 4.1EE526 pKa = 4.46AFQGLLYY533 pKa = 10.95SGGLVAGWVIKK544 pKa = 9.73DD545 pKa = 3.42ARR547 pKa = 11.84GGTTATYY554 pKa = 9.25PVTIIKK560 pKa = 9.93SYY562 pKa = 10.8RR563 pKa = 11.84GAASDD568 pKa = 3.49WQFAAPYY575 pKa = 8.57TKK577 pKa = 10.29DD578 pKa = 3.49YY579 pKa = 11.03VHH581 pKa = 8.31AEE583 pKa = 4.09MVFKK587 pKa = 9.87LTSVGATLLATTGPIGMSRR606 pKa = 11.84AKK608 pKa = 9.67WYY610 pKa = 10.48LSRR613 pKa = 11.84PTVPSDD619 pKa = 3.47YY620 pKa = 11.02QLDD623 pKa = 4.27ADD625 pKa = 4.48WKK627 pKa = 9.71AGPNAGGGPSGPPPPPPSQPPPEE650 pKa = 4.79PPGTPPDD657 pKa = 3.68YY658 pKa = 10.1STRR661 pKa = 11.84ASDD664 pKa = 4.26PPRR667 pKa = 11.84DD668 pKa = 3.89PNAPTDD674 pKa = 4.04DD675 pKa = 4.05VPPALTTTMGPDD687 pKa = 3.06TTTAALPHH695 pKa = 5.68SLKK698 pKa = 10.73LRR700 pKa = 11.84VEE702 pKa = 4.21TARR705 pKa = 11.84AALHH709 pKa = 7.0DD710 pKa = 5.01AGQSTDD716 pKa = 2.9WLEE719 pKa = 4.0SLTLGLQRR727 pKa = 11.84RR728 pKa = 11.84TTLSTPWEE736 pKa = 4.2DD737 pKa = 3.22RR738 pKa = 11.84DD739 pKa = 3.6RR740 pKa = 11.84EE741 pKa = 4.25GRR743 pKa = 11.84MRR745 pKa = 11.84AALDD749 pKa = 3.29GMQITDD755 pKa = 4.7PIEE758 pKa = 3.79MLKK761 pKa = 10.6AVPNGSRR768 pKa = 11.84ANVATCLAAAYY779 pKa = 8.56KK780 pKa = 10.51AAVPCAHH787 pKa = 6.76SLSAAHH793 pKa = 5.87VWAVEE798 pKa = 3.93AGRR801 pKa = 11.84MSSRR805 pKa = 11.84ARR807 pKa = 11.84ALRR810 pKa = 11.84ACSAMTRR817 pKa = 11.84VEE819 pKa = 4.1LEE821 pKa = 4.46DD822 pKa = 3.42YY823 pKa = 7.11TTRR826 pKa = 11.84HH827 pKa = 5.08AVKK830 pKa = 10.37NAALSVDD837 pKa = 4.01DD838 pKa = 4.83GSIAKK843 pKa = 10.35ALGRR847 pKa = 11.84GIAAKK852 pKa = 10.1EE853 pKa = 4.06LIGSAGKK860 pKa = 10.51RR861 pKa = 11.84SGDD864 pKa = 2.98KK865 pKa = 8.53WTADD869 pKa = 3.29PVIEE873 pKa = 4.65RR874 pKa = 11.84KK875 pKa = 9.34LAAPDD880 pKa = 3.92APSDD884 pKa = 4.45AISSDD889 pKa = 3.65TEE891 pKa = 4.46DD892 pKa = 3.81MIKK895 pKa = 9.71TSYY898 pKa = 10.91VDD900 pKa = 3.51EE901 pKa = 4.92QIDD904 pKa = 3.99DD905 pKa = 3.86EE906 pKa = 4.52MVIAILGYY914 pKa = 9.75APAWLRR920 pKa = 11.84QSSEE924 pKa = 4.09VPGTDD929 pKa = 2.86GDD931 pKa = 4.25EE932 pKa = 4.26EE933 pKa = 4.41QKK935 pKa = 11.06EE936 pKa = 4.34SDD938 pKa = 4.34PPDD941 pKa = 4.07PMDD944 pKa = 4.05LATTIGSVTARR955 pKa = 11.84VRR957 pKa = 11.84GRR959 pKa = 11.84SLEE962 pKa = 4.34DD963 pKa = 3.32MAKK966 pKa = 10.11HH967 pKa = 6.0APADD971 pKa = 3.47QRR973 pKa = 11.84LDD975 pKa = 3.44RR976 pKa = 11.84RR977 pKa = 11.84PVKK980 pKa = 10.65VAVSWDD986 pKa = 3.61TLNTVAQARR995 pKa = 11.84ARR997 pKa = 11.84SKK999 pKa = 10.82IEE1001 pKa = 4.15GGDD1004 pKa = 3.76VEE1006 pKa = 4.25VDD1008 pKa = 3.84GYY1010 pKa = 10.66LVRR1013 pKa = 11.84QDD1015 pKa = 3.78GSVEE1019 pKa = 4.0QISFNARR1026 pKa = 11.84GEE1028 pKa = 4.14AMHH1031 pKa = 6.87ARR1033 pKa = 11.84VEE1035 pKa = 4.58LITEE1039 pKa = 4.12EE1040 pKa = 4.56LDD1042 pKa = 3.21PHH1044 pKa = 5.8ITVSSVTAAPEE1055 pKa = 3.9ADD1057 pKa = 3.17KK1058 pKa = 11.29HH1059 pKa = 5.54VVTPAGTTVLEE1070 pKa = 4.16EE1071 pKa = 3.98QEE1073 pKa = 4.84AIADD1077 pKa = 3.66IAGSGTQTADD1087 pKa = 3.21FGLHH1091 pKa = 5.6TDD1093 pKa = 4.03ITPMLPPMPPPASSTASPTTTTTSQSHH1120 pKa = 6.32GKK1122 pKa = 8.44QSRR1125 pKa = 11.84TPTPKK1130 pKa = 9.89RR1131 pKa = 11.84QSSNAPPSDD1140 pKa = 3.17AQEE1143 pKa = 3.59IHH1145 pKa = 6.68RR1146 pKa = 11.84LVFKK1150 pKa = 10.67EE1151 pKa = 4.17QDD1153 pKa = 3.36DD1154 pKa = 4.09TT1155 pKa = 4.46

Molecular weight: 124.54 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	1155	1155	1155	1155.0	124.54

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
11.688 ± 0.0	1.039 ± 0.0	6.407 ± 0.0	4.675 ± 0.0	1.732 ± 0.0	6.667 ± 0.0	1.991 ± 0.0	3.983 ± 0.0	2.944 ± 0.0	8.918 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.251 ± 0.0	2.424 ± 0.0	8.139 ± 0.0	3.463 ± 0.0	5.887 ± 0.0	8.139 ± 0.0	7.965 ± 0.0	6.84 ± 0.0	2.078 ± 0.0	2.771 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski