Semliki forest virus (SFV)
Average proteome isoelectric point is 7.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P08411|POLN_SFV Polyprotein P1234 OS=Semliki forest virus OX=11033 PE=1 SV=2
MM1 pKa = 7.23 AAKK4 pKa = 10.06 VHH6 pKa = 5.91 VDD8 pKa = 3.51 IEE10 pKa = 4.4 ADD12 pKa = 3.65 SPFIKK17 pKa = 10.3 SLQKK21 pKa = 10.5 AFPSFEE27 pKa = 4.18 VEE29 pKa = 4.31 SLQVTPNDD37 pKa = 3.7 HH38 pKa = 6.54 ANARR42 pKa = 11.84 AFSHH46 pKa = 7.06 LATKK50 pKa = 10.64 LIEE53 pKa = 3.98 QEE55 pKa = 3.91 TDD57 pKa = 2.69 KK58 pKa = 11.15 DD59 pKa = 3.98 TLILDD64 pKa = 3.86 IGSAPSRR71 pKa = 11.84 RR72 pKa = 11.84 MMSTHH77 pKa = 7.32 KK78 pKa = 9.23 YY79 pKa = 9.86 HH80 pKa = 6.77 CVCPMRR86 pKa = 11.84 SAEE89 pKa = 4.02 DD90 pKa = 3.77 PEE92 pKa = 5.43 RR93 pKa = 11.84 LVCYY97 pKa = 10.05 AKK99 pKa = 10.67 KK100 pKa = 10.22 LAAASGKK107 pKa = 10.13 VLDD110 pKa = 4.57 RR111 pKa = 11.84 EE112 pKa = 4.24 IAGKK116 pKa = 8.37 ITDD119 pKa = 4.55 LQTVMATPDD128 pKa = 3.35 AEE130 pKa = 4.61 SPTFCLHH137 pKa = 6.47 TDD139 pKa = 3.67 VTCRR143 pKa = 11.84 TAAEE147 pKa = 4.04 VAVYY151 pKa = 9.92 QDD153 pKa = 3.71 VYY155 pKa = 11.3 AVHH158 pKa = 7.13 APTSLYY164 pKa = 8.74 HH165 pKa = 5.53 QAMKK169 pKa = 10.56 GVRR172 pKa = 11.84 TAYY175 pKa = 9.57 WIGFDD180 pKa = 3.41 TTPFMFDD187 pKa = 3.6 ALAGAYY193 pKa = 7.07 PTYY196 pKa = 9.32 ATNWADD202 pKa = 3.71 EE203 pKa = 4.23 QVLQARR209 pKa = 11.84 NIGLCAASLTEE220 pKa = 3.92 GRR222 pKa = 11.84 LGKK225 pKa = 10.6 LSILRR230 pKa = 11.84 KK231 pKa = 9.59 KK232 pKa = 8.34 QLKK235 pKa = 9.77 PCDD238 pKa = 3.22 TVMFSVGSTLYY249 pKa = 10.03 TEE251 pKa = 4.25 SRR253 pKa = 11.84 KK254 pKa = 9.79 LLRR257 pKa = 11.84 SWHH260 pKa = 6.11 LPSVFHH266 pKa = 6.91 LKK268 pKa = 10.36 GKK270 pKa = 10.29 QSFTCRR276 pKa = 11.84 CDD278 pKa = 3.56 TIVSCEE284 pKa = 4.06 GYY286 pKa = 9.24 VVKK289 pKa = 10.51 KK290 pKa = 9.56 ITMCPGLYY298 pKa = 10.36 GKK300 pKa = 7.94 TVGYY304 pKa = 10.56 AVTYY308 pKa = 9.17 HH309 pKa = 7.04 AEE311 pKa = 3.99 GFLVCKK317 pKa = 8.49 TTDD320 pKa = 3.15 TVKK323 pKa = 10.8 GEE325 pKa = 3.97 RR326 pKa = 11.84 VSFPVCTYY334 pKa = 10.42 VPSTICDD341 pKa = 3.17 QMTGILATDD350 pKa = 3.81 VTPEE354 pKa = 4.08 DD355 pKa = 3.7 AQKK358 pKa = 11.43 LLVGLNQRR366 pKa = 11.84 IVVNGRR372 pKa = 11.84 TQRR375 pKa = 11.84 NTNTMKK381 pKa = 10.77 NYY383 pKa = 9.87 LLPIVAVAFSKK394 pKa = 10.07 WARR397 pKa = 11.84 EE398 pKa = 3.97 YY399 pKa = 11.11 KK400 pKa = 10.46 ADD402 pKa = 5.03 LDD404 pKa = 4.13 DD405 pKa = 4.76 EE406 pKa = 4.82 KK407 pKa = 11.17 PLGVRR412 pKa = 11.84 EE413 pKa = 4.24 RR414 pKa = 11.84 SLTCCCLWAFKK425 pKa = 8.5 TRR427 pKa = 11.84 KK428 pKa = 7.16 MHH430 pKa = 5.9 TMYY433 pKa = 10.75 KK434 pKa = 10.3 KK435 pKa = 10.23 PDD437 pKa = 3.19 TQTIVKK443 pKa = 9.62 VPSEE447 pKa = 4.24 FNSFVIPSLWSTGLAIPVRR466 pKa = 11.84 SRR468 pKa = 11.84 IKK470 pKa = 9.99 MLLAKK475 pKa = 8.89 KK476 pKa = 8.15 TKK478 pKa = 10.21 RR479 pKa = 11.84 EE480 pKa = 4.4 LIPVLDD486 pKa = 4.13 ASSARR491 pKa = 11.84 DD492 pKa = 3.34 AEE494 pKa = 4.29 QEE496 pKa = 3.96 EE497 pKa = 4.73 KK498 pKa = 10.81 EE499 pKa = 4.09 RR500 pKa = 11.84 LEE502 pKa = 5.02 AEE504 pKa = 4.47 LTRR507 pKa = 11.84 EE508 pKa = 4.15 ALPPLVPIAPAEE520 pKa = 4.2 TGVVDD525 pKa = 3.64 VDD527 pKa = 3.95 VEE529 pKa = 4.15 EE530 pKa = 4.93 LEE532 pKa = 4.22 YY533 pKa = 10.77 HH534 pKa = 6.51 AGAGVVEE541 pKa = 4.79 TPRR544 pKa = 11.84 SALKK548 pKa = 9.32 VTAQPNDD555 pKa = 3.56 VLLGNYY561 pKa = 8.74 VVLSPQTVLKK571 pKa = 10.3 SSKK574 pKa = 9.66 LAPVHH579 pKa = 6.33 PLAEE583 pKa = 3.93 QVKK586 pKa = 9.55 IITHH590 pKa = 5.83 NGRR593 pKa = 11.84 AGRR596 pKa = 11.84 YY597 pKa = 7.55 QVDD600 pKa = 4.3 GYY602 pKa = 10.79 DD603 pKa = 3.68 GRR605 pKa = 11.84 VLLPCGSAIPVPEE618 pKa = 4.17 FQALSEE624 pKa = 4.4 SATMVYY630 pKa = 10.35 NEE632 pKa = 4.29 RR633 pKa = 11.84 EE634 pKa = 3.94 FVNRR638 pKa = 11.84 KK639 pKa = 8.6 LYY641 pKa = 10.49 HH642 pKa = 6.12 IAVHH646 pKa = 6.41 GPSLNTDD653 pKa = 3.18 EE654 pKa = 4.84 EE655 pKa = 4.67 NYY657 pKa = 10.34 EE658 pKa = 3.99 KK659 pKa = 10.92 VRR661 pKa = 11.84 AEE663 pKa = 4.04 RR664 pKa = 11.84 TDD666 pKa = 3.05 AEE668 pKa = 4.68 YY669 pKa = 11.44 VFDD672 pKa = 4.53 VDD674 pKa = 5.45 KK675 pKa = 10.84 KK676 pKa = 9.22 CCVKK680 pKa = 10.6 RR681 pKa = 11.84 EE682 pKa = 4.03 EE683 pKa = 4.17 ASGLVLVGEE692 pKa = 4.44 LTNPPFHH699 pKa = 6.66 EE700 pKa = 4.38 FAYY703 pKa = 9.79 EE704 pKa = 3.92 GLKK707 pKa = 9.91 IRR709 pKa = 11.84 PSAPYY714 pKa = 7.96 KK715 pKa = 7.34 TTVVGVFGVPGSGKK729 pKa = 9.82 SAIIKK734 pKa = 10.36 SLVTKK739 pKa = 10.42 HH740 pKa = 6.94 DD741 pKa = 4.03 LVTSGKK747 pKa = 10.06 KK748 pKa = 9.65 EE749 pKa = 3.77 NCQEE753 pKa = 3.73 IVNDD757 pKa = 3.88 VKK759 pKa = 10.98 KK760 pKa = 10.29 HH761 pKa = 5.86 RR762 pKa = 11.84 GLDD765 pKa = 3.49 IQAKK769 pKa = 7.54 TVDD772 pKa = 4.04 SILLNGCRR780 pKa = 11.84 RR781 pKa = 11.84 AVDD784 pKa = 3.14 ILYY787 pKa = 10.27 VDD789 pKa = 3.87 EE790 pKa = 6.07 AFACHH795 pKa = 6.76 SGTLLALIALVKK807 pKa = 10.09 PRR809 pKa = 11.84 SKK811 pKa = 10.93 VVLCGDD817 pKa = 4.33 PKK819 pKa = 10.69 QCGFFNMMQLKK830 pKa = 10.72 VNFNHH835 pKa = 6.98 NICTEE840 pKa = 4.09 VCHH843 pKa = 6.6 KK844 pKa = 10.51 SISRR848 pKa = 11.84 RR849 pKa = 11.84 CTRR852 pKa = 11.84 PVTAIVSTLHH862 pKa = 6.15 YY863 pKa = 8.59 GGKK866 pKa = 8.54 MRR868 pKa = 11.84 TTNPCNKK875 pKa = 9.52 PIIIDD880 pKa = 3.46 TTGQTKK886 pKa = 9.88 PKK888 pKa = 9.8 PGDD891 pKa = 3.18 IVLTCFRR898 pKa = 11.84 GWVKK902 pKa = 10.19 QLQLDD907 pKa = 4.14 YY908 pKa = 10.75 RR909 pKa = 11.84 GHH911 pKa = 5.99 EE912 pKa = 4.46 VMTAAASQGLTRR924 pKa = 11.84 KK925 pKa = 9.35 GVYY928 pKa = 9.7 AVRR931 pKa = 11.84 QKK933 pKa = 11.2 VNEE936 pKa = 4.08 NPLYY940 pKa = 10.75 APASEE945 pKa = 4.39 HH946 pKa = 6.37 VNVLLTRR953 pKa = 11.84 TEE955 pKa = 4.6 DD956 pKa = 3.39 RR957 pKa = 11.84 LVWKK961 pKa = 8.62 TLAGDD966 pKa = 3.55 PWIKK970 pKa = 10.52 VLSNIPQGNFTATLEE985 pKa = 4.22 EE986 pKa = 4.35 WQEE989 pKa = 3.85 EE990 pKa = 3.97 HH991 pKa = 7.2 DD992 pKa = 4.81 KK993 pKa = 10.82 IMKK996 pKa = 9.93 VIEE999 pKa = 4.63 GPAAPVDD1006 pKa = 3.69 AFQNKK1011 pKa = 9.99 ANVCWAKK1018 pKa = 10.88 SLVPVLDD1025 pKa = 3.54 TAGIRR1030 pKa = 11.84 LTAEE1034 pKa = 3.76 EE1035 pKa = 4.66 WSTIITAFKK1044 pKa = 10.08 EE1045 pKa = 3.75 DD1046 pKa = 3.37 RR1047 pKa = 11.84 AYY1049 pKa = 11.34 SPVVALNEE1057 pKa = 4.04 ICTKK1061 pKa = 10.69 YY1062 pKa = 10.96 YY1063 pKa = 10.85 GVDD1066 pKa = 3.45 LDD1068 pKa = 4.44 SGLFSAPKK1076 pKa = 9.6 VSLYY1080 pKa = 10.84 YY1081 pKa = 10.64 EE1082 pKa = 4.16 NNHH1085 pKa = 5.6 WDD1087 pKa = 3.5 NRR1089 pKa = 11.84 PGGRR1093 pKa = 11.84 MYY1095 pKa = 11.26 GFNAATAARR1104 pKa = 11.84 LEE1106 pKa = 4.13 ARR1108 pKa = 11.84 HH1109 pKa = 5.76 TFLKK1113 pKa = 9.85 GQWHH1117 pKa = 6.09 TGKK1120 pKa = 9.98 QAVIAEE1126 pKa = 4.36 RR1127 pKa = 11.84 KK1128 pKa = 8.5 IQPLSVLDD1136 pKa = 3.61 NVIPINRR1143 pKa = 11.84 RR1144 pKa = 11.84 LPHH1147 pKa = 6.57 ALVAEE1152 pKa = 4.27 YY1153 pKa = 8.56 KK1154 pKa = 8.37 TVKK1157 pKa = 9.98 GSRR1160 pKa = 11.84 VEE1162 pKa = 3.79 WLVNKK1167 pKa = 9.64 VRR1169 pKa = 11.84 GYY1171 pKa = 10.55 HH1172 pKa = 5.14 VLLVSEE1178 pKa = 4.81 YY1179 pKa = 11.04 NLALPRR1185 pKa = 11.84 RR1186 pKa = 11.84 RR1187 pKa = 11.84 VTWLSPLNVTGADD1200 pKa = 3.01 RR1201 pKa = 11.84 CYY1203 pKa = 10.89 DD1204 pKa = 3.78 LSLGLPADD1212 pKa = 3.92 AGRR1215 pKa = 11.84 FDD1217 pKa = 4.5 LVFVNIHH1224 pKa = 4.31 TEE1226 pKa = 4.0 FRR1228 pKa = 11.84 IHH1230 pKa = 7.58 HH1231 pKa = 5.41 YY1232 pKa = 7.31 QQCVDD1237 pKa = 3.83 HH1238 pKa = 6.91 AMKK1241 pKa = 10.65 LQMLGGDD1248 pKa = 4.2 ALRR1251 pKa = 11.84 LLKK1254 pKa = 10.65 PGGSLLMRR1262 pKa = 11.84 AYY1264 pKa = 10.4 GYY1266 pKa = 11.07 ADD1268 pKa = 4.41 KK1269 pKa = 10.72 ISEE1272 pKa = 4.33 AVVSSLSRR1280 pKa = 11.84 KK1281 pKa = 8.87 FSSARR1286 pKa = 11.84 VLRR1289 pKa = 11.84 PDD1291 pKa = 3.49 CVTSNTEE1298 pKa = 3.76 VFLLFSNFDD1307 pKa = 3.31 NGKK1310 pKa = 9.74 RR1311 pKa = 11.84 PSTLHH1316 pKa = 5.61 QMNTKK1321 pKa = 10.39 LSAVYY1326 pKa = 10.1 AGEE1329 pKa = 4.06 AMHH1332 pKa = 6.72 TAGCAPSYY1340 pKa = 9.56 RR1341 pKa = 11.84 VKK1343 pKa = 10.66 RR1344 pKa = 11.84 ADD1346 pKa = 2.92 IATCTEE1352 pKa = 3.92 AAVVNAANARR1362 pKa = 11.84 GTVGDD1367 pKa = 4.28 GVCRR1371 pKa = 11.84 AVAKK1375 pKa = 9.9 KK1376 pKa = 9.48 WPSAFKK1382 pKa = 10.75 GAATPVGTIKK1392 pKa = 9.95 TVMCGSYY1399 pKa = 10.31 PVIHH1403 pKa = 6.65 AVAPNFSATTEE1414 pKa = 4.15 AEE1416 pKa = 4.08 GDD1418 pKa = 3.59 RR1419 pKa = 11.84 EE1420 pKa = 3.87 LAAVYY1425 pKa = 9.82 RR1426 pKa = 11.84 AVAAEE1431 pKa = 4.03 VNRR1434 pKa = 11.84 LSLSSVAIPLLSTGVFSGGRR1454 pKa = 11.84 DD1455 pKa = 3.02 RR1456 pKa = 11.84 LQQSLNHH1463 pKa = 6.74 LFTAMDD1469 pKa = 3.6 ATDD1472 pKa = 4.21 ADD1474 pKa = 3.8 VTIYY1478 pKa = 10.82 CRR1480 pKa = 11.84 DD1481 pKa = 3.47 KK1482 pKa = 10.78 SWEE1485 pKa = 4.05 KK1486 pKa = 10.56 KK1487 pKa = 8.41 IQEE1490 pKa = 4.7 AIDD1493 pKa = 3.35 MRR1495 pKa = 11.84 TAVEE1499 pKa = 4.23 LLNDD1503 pKa = 4.02 DD1504 pKa = 4.63 VEE1506 pKa = 4.51 LTTDD1510 pKa = 4.22 LVRR1513 pKa = 11.84 VHH1515 pKa = 7.22 PDD1517 pKa = 2.53 SSLVGRR1523 pKa = 11.84 KK1524 pKa = 9.54 GYY1526 pKa = 8.43 STTDD1530 pKa = 3.0 GSLYY1534 pKa = 10.63 SYY1536 pKa = 11.4 FEE1538 pKa = 4.03 GTKK1541 pKa = 10.16 FNQAAIDD1548 pKa = 3.62 MAEE1551 pKa = 4.9 ILTLWPRR1558 pKa = 11.84 LQEE1561 pKa = 4.21 ANEE1564 pKa = 4.11 QICLYY1569 pKa = 11.0 ALGEE1573 pKa = 4.2 TMDD1576 pKa = 4.97 NIRR1579 pKa = 11.84 SKK1581 pKa = 11.37 CPVNDD1586 pKa = 4.19 SDD1588 pKa = 5.14 SSTPPRR1594 pKa = 11.84 TVPCLCRR1601 pKa = 11.84 YY1602 pKa = 10.52 AMTAEE1607 pKa = 4.69 RR1608 pKa = 11.84 IARR1611 pKa = 11.84 LRR1613 pKa = 11.84 SHH1615 pKa = 5.78 QVKK1618 pKa = 10.83 SMVVCSSFPLPKK1630 pKa = 9.88 YY1631 pKa = 10.39 HH1632 pKa = 7.21 VDD1634 pKa = 3.16 GVQKK1638 pKa = 9.98 VKK1640 pKa = 10.87 CEE1642 pKa = 3.82 KK1643 pKa = 10.81 GLLFDD1648 pKa = 3.74 PTVPSVVSPRR1658 pKa = 11.84 KK1659 pKa = 9.08 YY1660 pKa = 9.92 AASTTDD1666 pKa = 2.7 HH1667 pKa = 7.18 SDD1669 pKa = 3.05 RR1670 pKa = 11.84 SLRR1673 pKa = 11.84 GFDD1676 pKa = 5.28 LDD1678 pKa = 3.33 WTTDD1682 pKa = 3.36 SSSTASDD1689 pKa = 3.5 TMSLPSLQSCDD1700 pKa = 2.67 IDD1702 pKa = 4.81 SIYY1705 pKa = 11.04 EE1706 pKa = 3.7 PMAPIVVTADD1716 pKa = 3.19 VHH1718 pKa = 6.11 PEE1720 pKa = 3.55 PAGIADD1726 pKa = 4.74 LAADD1730 pKa = 3.93 VHH1732 pKa = 6.51 PEE1734 pKa = 3.94 PADD1737 pKa = 3.56 HH1738 pKa = 7.25 VDD1740 pKa = 4.17 LEE1742 pKa = 4.57 NPIPPPRR1749 pKa = 11.84 PKK1751 pKa = 10.1 RR1752 pKa = 11.84 AAYY1755 pKa = 9.15 LASRR1759 pKa = 11.84 AAEE1762 pKa = 3.89 RR1763 pKa = 11.84 PVPAPRR1769 pKa = 11.84 KK1770 pKa = 6.39 PTPAPRR1776 pKa = 11.84 TAFRR1780 pKa = 11.84 NKK1782 pKa = 10.32 LPLTFGDD1789 pKa = 3.9 FDD1791 pKa = 3.71 EE1792 pKa = 6.23 HH1793 pKa = 6.77 EE1794 pKa = 4.31 VDD1796 pKa = 4.77 ALASGITFGDD1806 pKa = 3.4 FDD1808 pKa = 4.35 DD1809 pKa = 3.87 VLRR1812 pKa = 11.84 LGRR1815 pKa = 11.84 AGAYY1819 pKa = 9.31 IFSSDD1824 pKa = 3.4 TGSGHH1829 pKa = 6.97 LQQKK1833 pKa = 7.61 SVRR1836 pKa = 11.84 QHH1838 pKa = 6.07 NLQCAQLDD1846 pKa = 3.81 AVEE1849 pKa = 4.3 EE1850 pKa = 4.2 EE1851 pKa = 4.39 KK1852 pKa = 10.26 MYY1854 pKa = 10.41 PPKK1857 pKa = 10.63 LDD1859 pKa = 3.44 TEE1861 pKa = 4.32 RR1862 pKa = 11.84 EE1863 pKa = 4.0 KK1864 pKa = 11.42 LLLLKK1869 pKa = 9.81 MQMHH1873 pKa = 7.16 PSEE1876 pKa = 4.58 ANKK1879 pKa = 10.19 SRR1881 pKa = 11.84 YY1882 pKa = 7.27 QSRR1885 pKa = 11.84 KK1886 pKa = 7.62 VEE1888 pKa = 4.03 NMKK1891 pKa = 9.67 ATVVDD1896 pKa = 4.27 RR1897 pKa = 11.84 LTSGARR1903 pKa = 11.84 LYY1905 pKa = 10.44 TGADD1909 pKa = 3.16 VGRR1912 pKa = 11.84 IPTYY1916 pKa = 9.62 AVRR1919 pKa = 11.84 YY1920 pKa = 5.92 PRR1922 pKa = 11.84 PVYY1925 pKa = 10.41 SPTVIEE1931 pKa = 4.73 RR1932 pKa = 11.84 FSSPDD1937 pKa = 3.21 VAIAACNEE1945 pKa = 4.08 YY1946 pKa = 10.71 LSRR1949 pKa = 11.84 NYY1951 pKa = 8.4 PTVASYY1957 pKa = 10.99 QITDD1961 pKa = 3.64 EE1962 pKa = 4.04 YY1963 pKa = 11.39 DD1964 pKa = 3.05 AYY1966 pKa = 11.6 LDD1968 pKa = 3.91 MVDD1971 pKa = 5.75 GSDD1974 pKa = 3.51 SCLDD1978 pKa = 3.32 RR1979 pKa = 11.84 ATFCPAKK1986 pKa = 10.1 LRR1988 pKa = 11.84 CYY1990 pKa = 8.97 PKK1992 pKa = 9.72 HH1993 pKa = 6.07 HH1994 pKa = 7.36 AYY1996 pKa = 9.69 HH1997 pKa = 5.9 QPTVRR2002 pKa = 11.84 SAVPSPFQNTLQNVLAAATKK2022 pKa = 9.91 RR2023 pKa = 11.84 NCNVTQMRR2031 pKa = 11.84 EE2032 pKa = 4.17 LPTMDD2037 pKa = 3.1 SAVFNVEE2044 pKa = 3.6 CFKK2047 pKa = 10.9 RR2048 pKa = 11.84 YY2049 pKa = 9.7 ACSGEE2054 pKa = 3.93 YY2055 pKa = 9.07 WEE2057 pKa = 5.88 EE2058 pKa = 4.15 YY2059 pKa = 9.73 AKK2061 pKa = 10.52 QPIRR2065 pKa = 11.84 ITTEE2069 pKa = 3.66 NITTYY2074 pKa = 8.1 VTKK2077 pKa = 10.96 LKK2079 pKa = 10.51 GPKK2082 pKa = 9.28 AAALFAKK2089 pKa = 8.13 THH2091 pKa = 5.99 NLVPLQEE2098 pKa = 4.17 VPMDD2102 pKa = 3.95 RR2103 pKa = 11.84 FTVDD2107 pKa = 2.78 MKK2109 pKa = 11.01 RR2110 pKa = 11.84 DD2111 pKa = 3.64 VKK2113 pKa = 9.76 VTPGTKK2119 pKa = 8.49 HH2120 pKa = 4.48 TEE2122 pKa = 3.91 EE2123 pKa = 4.12 RR2124 pKa = 11.84 PKK2126 pKa = 10.92 VQVIQAAEE2134 pKa = 3.99 PLATAYY2140 pKa = 10.83 LCGIHH2145 pKa = 6.74 RR2146 pKa = 11.84 EE2147 pKa = 4.01 LVRR2150 pKa = 11.84 RR2151 pKa = 11.84 LNAVLRR2157 pKa = 11.84 PNVHH2161 pKa = 6.01 TLFDD2165 pKa = 3.67 MSAEE2169 pKa = 4.19 DD2170 pKa = 3.84 FDD2172 pKa = 6.61 AIIASHH2178 pKa = 6.31 FHH2180 pKa = 6.64 PGDD2183 pKa = 3.57 PVLEE2187 pKa = 3.96 TDD2189 pKa = 3.41 IASFDD2194 pKa = 3.58 KK2195 pKa = 11.43 SQDD2198 pKa = 3.29 DD2199 pKa = 3.96 SLALTGLMILEE2210 pKa = 4.67 DD2211 pKa = 4.28 LGVDD2215 pKa = 3.88 QYY2217 pKa = 12.13 LLDD2220 pKa = 5.14 LIEE2223 pKa = 4.37 AAFGEE2228 pKa = 4.67 ISSCHH2233 pKa = 6.67 LPTGTRR2239 pKa = 11.84 FKK2241 pKa = 10.46 FGAMMKK2247 pKa = 10.15 SGMFLTLFINTVLNITIASRR2267 pKa = 11.84 VLEE2270 pKa = 3.91 QRR2272 pKa = 11.84 LTDD2275 pKa = 3.72 SACAAFIGDD2284 pKa = 4.09 DD2285 pKa = 4.12 NIVHH2289 pKa = 6.37 GVISDD2294 pKa = 3.24 KK2295 pKa = 11.39 LMAEE2299 pKa = 4.95 RR2300 pKa = 11.84 CASWVNMEE2308 pKa = 4.29 VKK2310 pKa = 10.31 IIDD2313 pKa = 3.47 AVMGEE2318 pKa = 4.34 KK2319 pKa = 10.1 PPYY2322 pKa = 8.65 FCGGFIVFDD2331 pKa = 4.12 SVTQTACRR2339 pKa = 11.84 VSDD2342 pKa = 3.64 PLKK2345 pKa = 10.81 RR2346 pKa = 11.84 LFKK2349 pKa = 10.55 LGKK2352 pKa = 9.2 PLTAEE2357 pKa = 4.49 DD2358 pKa = 4.2 KK2359 pKa = 10.5 QDD2361 pKa = 3.36 EE2362 pKa = 4.31 DD2363 pKa = 3.63 RR2364 pKa = 11.84 RR2365 pKa = 11.84 RR2366 pKa = 11.84 ALSDD2370 pKa = 3.64 EE2371 pKa = 3.9 VSKK2374 pKa = 10.54 WFRR2377 pKa = 11.84 TGLGAEE2383 pKa = 4.45 LEE2385 pKa = 4.48 VALTSRR2391 pKa = 11.84 YY2392 pKa = 8.32 EE2393 pKa = 3.95 VEE2395 pKa = 4.09 GCKK2398 pKa = 10.54 SILIAMATLARR2409 pKa = 11.84 DD2410 pKa = 3.3 IKK2412 pKa = 10.9 AFKK2415 pKa = 10.14 KK2416 pKa = 10.37 LRR2418 pKa = 11.84 GPVIHH2423 pKa = 7.18 LYY2425 pKa = 10.45 GGPRR2429 pKa = 11.84 LVRR2432 pKa = 3.89
Molecular weight: 269.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.929
IPC2_protein 7.293
IPC_protein 7.059
Toseland 6.825
ProMoST 7.732
Dawson 7.775
Bjellqvist 8.258
Wikipedia 7.673
Rodwell 7.805
Grimsley 6.912
Solomon 7.775
Lehninger 7.805
Nozaki 8.492
DTASelect 7.951
Thurlkill 8.009
EMBOSS 7.995
Sillero 8.346
Patrickios 4.266
IPC_peptide 7.775
IPC2_peptide 7.351
IPC2.peptide.svr19 7.235
Protein with the highest isoelectric point:
>sp|P08411|POLN_SFV Polyprotein P1234 OS=Semliki forest virus OX=11033 PE=1 SV=2
MM1 pKa = 7.57 NYY3 pKa = 9.73 IPTQTFYY10 pKa = 11.22 GRR12 pKa = 11.84 RR13 pKa = 11.84 WRR15 pKa = 11.84 PRR17 pKa = 11.84 PAARR21 pKa = 11.84 PWPLQATPVAPVVPDD36 pKa = 4.5 FQAQQMQQLISAVNALTMRR55 pKa = 11.84 QNAIAPARR63 pKa = 11.84 PPKK66 pKa = 9.73 PKK68 pKa = 9.89 KK69 pKa = 10.42 KK70 pKa = 9.2 KK71 pKa = 4.82 TTKK74 pKa = 9.93 PKK76 pKa = 10.59 PKK78 pKa = 8.95 TQPKK82 pKa = 9.21 KK83 pKa = 10.74 INGKK87 pKa = 6.47 TQQQKK92 pKa = 10.93 KK93 pKa = 9.73 KK94 pKa = 10.68 DD95 pKa = 3.53 KK96 pKa = 10.97 QADD99 pKa = 3.66 KK100 pKa = 10.94 KK101 pKa = 10.57 KK102 pKa = 10.03 KK103 pKa = 10.11 KK104 pKa = 8.28 PGKK107 pKa = 9.32 RR108 pKa = 11.84 EE109 pKa = 3.84 RR110 pKa = 11.84 MCMKK114 pKa = 10.12 IEE116 pKa = 3.99 NDD118 pKa = 4.0 CIFEE122 pKa = 4.27 VKK124 pKa = 10.55 HH125 pKa = 5.59 EE126 pKa = 4.41 GKK128 pKa = 8.61 VTGYY132 pKa = 10.98 ACLVGDD138 pKa = 4.21 KK139 pKa = 10.61 VMKK142 pKa = 8.7 PAHH145 pKa = 5.82 VKK147 pKa = 10.64 GVIDD151 pKa = 3.79 NADD154 pKa = 3.68 LAKK157 pKa = 10.68 LAFKK161 pKa = 10.65 KK162 pKa = 10.39 SSKK165 pKa = 10.6 YY166 pKa = 10.35 DD167 pKa = 3.62 LEE169 pKa = 4.45 CAQIPVHH176 pKa = 5.68 MRR178 pKa = 11.84 SDD180 pKa = 3.26 ASKK183 pKa = 8.2 YY184 pKa = 6.47 THH186 pKa = 6.76 EE187 pKa = 4.89 KK188 pKa = 10.56 PEE190 pKa = 3.88 GHH192 pKa = 6.34 YY193 pKa = 9.58 NWHH196 pKa = 6.8 HH197 pKa = 6.34 GAVQYY202 pKa = 11.02 SGGRR206 pKa = 11.84 FTIPTGAGKK215 pKa = 9.46 PGDD218 pKa = 3.72 SGRR221 pKa = 11.84 PIFDD225 pKa = 3.06 NKK227 pKa = 9.91 GRR229 pKa = 11.84 VVAIVLGGANEE240 pKa = 4.36 GSRR243 pKa = 11.84 TALSVVTWNKK253 pKa = 11.14 DD254 pKa = 2.71 MVTRR258 pKa = 11.84 VTPEE262 pKa = 3.7 GSEE265 pKa = 4.03 EE266 pKa = 3.74 WSAPLITAMCVLANATFPCFQPPCVPCCYY295 pKa = 10.03 EE296 pKa = 4.03 NNAEE300 pKa = 3.98 ATLRR304 pKa = 11.84 MLEE307 pKa = 4.44 DD308 pKa = 3.5 NVDD311 pKa = 3.39 RR312 pKa = 11.84 PGYY315 pKa = 10.45 YY316 pKa = 10.31 DD317 pKa = 4.58 LLQAALTCRR326 pKa = 11.84 NGTRR330 pKa = 11.84 HH331 pKa = 5.8 RR332 pKa = 11.84 RR333 pKa = 11.84 SVSQHH338 pKa = 4.65 FNVYY342 pKa = 9.9 KK343 pKa = 10.03 ATRR346 pKa = 11.84 PYY348 pKa = 10.02 IAYY351 pKa = 10.04 CADD354 pKa = 3.51 CGAGHH359 pKa = 6.46 SCHH362 pKa = 6.81 SPVAIEE368 pKa = 4.08 AVRR371 pKa = 11.84 SEE373 pKa = 4.33 ATDD376 pKa = 3.33 GMLKK380 pKa = 9.73 IQFSAQIGIDD390 pKa = 3.02 KK391 pKa = 10.61 SDD393 pKa = 3.05 NHH395 pKa = 7.4 DD396 pKa = 3.57 YY397 pKa = 11.22 TKK399 pKa = 10.33 IRR401 pKa = 11.84 YY402 pKa = 8.89 ADD404 pKa = 3.14 GHH406 pKa = 6.61 AIEE409 pKa = 4.3 NAVRR413 pKa = 11.84 SSLKK417 pKa = 10.13 VATSGDD423 pKa = 4.2 CFVHH427 pKa = 5.62 GTMGHH432 pKa = 6.92 FILAKK437 pKa = 9.87 CPPGEE442 pKa = 3.91 FLQVSIQDD450 pKa = 3.12 TRR452 pKa = 11.84 NAVRR456 pKa = 11.84 ACRR459 pKa = 11.84 IQYY462 pKa = 9.47 HH463 pKa = 7.02 HH464 pKa = 7.76 DD465 pKa = 3.69 PQPVGRR471 pKa = 11.84 EE472 pKa = 3.67 KK473 pKa = 10.26 FTIRR477 pKa = 11.84 PHH479 pKa = 5.64 YY480 pKa = 10.09 GKK482 pKa = 10.36 EE483 pKa = 4.26 IPCTTYY489 pKa = 10.56 QQTTAEE495 pKa = 4.3 TVEE498 pKa = 4.69 EE499 pKa = 4.81 IDD501 pKa = 3.15 MHH503 pKa = 6.15 MPPDD507 pKa = 3.96 TPDD510 pKa = 3.18 RR511 pKa = 11.84 TLLSQQSGNVKK522 pKa = 8.56 ITVGGKK528 pKa = 8.7 KK529 pKa = 9.85 VKK531 pKa = 10.44 YY532 pKa = 10.23 NCTCGTGNVGTTNSDD547 pKa = 3.17 MTINTCLIEE556 pKa = 4.06 QCHH559 pKa = 5.71 VSVTDD564 pKa = 3.47 HH565 pKa = 6.9 KK566 pKa = 10.65 KK567 pKa = 8.74 WQFNSPFVPRR577 pKa = 11.84 ADD579 pKa = 3.41 EE580 pKa = 3.93 PARR583 pKa = 11.84 KK584 pKa = 9.45 GKK586 pKa = 8.83 VHH588 pKa = 7.01 IPFPLDD594 pKa = 3.73 NITCRR599 pKa = 11.84 VPMARR604 pKa = 11.84 EE605 pKa = 3.63 PTVIHH610 pKa = 6.73 GKK612 pKa = 10.14 RR613 pKa = 11.84 EE614 pKa = 4.27 VTLHH618 pKa = 6.31 LHH620 pKa = 6.88 PDD622 pKa = 3.19 HH623 pKa = 6.99 PTLFSYY629 pKa = 8.72 RR630 pKa = 11.84 TLGEE634 pKa = 4.09 DD635 pKa = 3.51 PQYY638 pKa = 10.93 HH639 pKa = 5.81 EE640 pKa = 4.77 EE641 pKa = 4.19 WVTAAVEE648 pKa = 4.08 RR649 pKa = 11.84 TIPVPVDD656 pKa = 2.65 GMEE659 pKa = 4.04 YY660 pKa = 9.84 HH661 pKa = 7.16 WGNNDD666 pKa = 3.59 PVRR669 pKa = 11.84 LWSQLTTEE677 pKa = 5.27 GKK679 pKa = 8.23 PHH681 pKa = 6.75 GWPHH685 pKa = 6.65 QIVQYY690 pKa = 10.99 YY691 pKa = 8.98 YY692 pKa = 10.96 GLYY695 pKa = 9.55 PAATVSAVVGMSLLALISIFASCYY719 pKa = 7.8 MLVAARR725 pKa = 11.84 SKK727 pKa = 10.98 CLTPYY732 pKa = 11.25 ALTPGAAVPWTLGILCCAPRR752 pKa = 11.84 AHH754 pKa = 6.76 AASVAEE760 pKa = 4.11 TMAYY764 pKa = 10.36 LWDD767 pKa = 3.63 QNQALFWLEE776 pKa = 3.44 FAAPVACILIITYY789 pKa = 8.79 CLRR792 pKa = 11.84 NVLCCCKK799 pKa = 10.39 SLSFLVLLSLGATARR814 pKa = 11.84 AYY816 pKa = 9.2 EE817 pKa = 4.51 HH818 pKa = 6.14 STVMPNVVGFPYY830 pKa = 9.7 KK831 pKa = 10.72 AHH833 pKa = 6.97 IEE835 pKa = 4.17 RR836 pKa = 11.84 PGYY839 pKa = 10.49 SPLTLQMQVVEE850 pKa = 4.57 TSLEE854 pKa = 3.89 PTLNLEE860 pKa = 4.93 YY861 pKa = 9.64 ITCEE865 pKa = 4.01 YY866 pKa = 8.89 KK867 pKa = 10.34 TVVPSPYY874 pKa = 10.04 VKK876 pKa = 10.44 CCGASEE882 pKa = 4.58 CSTKK886 pKa = 10.47 EE887 pKa = 4.07 KK888 pKa = 10.25 PDD890 pKa = 3.82 YY891 pKa = 9.37 QCKK894 pKa = 10.31 VYY896 pKa = 10.29 TGVYY900 pKa = 8.44 PFMWGGAYY908 pKa = 9.35 CFCDD912 pKa = 3.74 SEE914 pKa = 4.29 NTQLSEE920 pKa = 4.39 AYY922 pKa = 9.93 VDD924 pKa = 3.72 RR925 pKa = 11.84 SDD927 pKa = 4.22 VCRR930 pKa = 11.84 HH931 pKa = 5.59 DD932 pKa = 3.53 HH933 pKa = 6.08 ASAYY937 pKa = 8.88 KK938 pKa = 10.52 AHH940 pKa = 6.24 TASLKK945 pKa = 9.92 AKK947 pKa = 9.38 VRR949 pKa = 11.84 VMYY952 pKa = 10.86 GNVNQTVDD960 pKa = 3.35 VYY962 pKa = 11.88 VNGDD966 pKa = 3.5 HH967 pKa = 7.05 AVTIGGTQFIFGPLSSAWTPFDD989 pKa = 3.59 NKK991 pKa = 10.09 IVVYY995 pKa = 10.06 KK996 pKa = 10.99 DD997 pKa = 3.19 EE998 pKa = 4.67 VFNQDD1003 pKa = 3.32 FPPYY1007 pKa = 10.63 GSGQPGRR1014 pKa = 11.84 FGDD1017 pKa = 3.53 IQSRR1021 pKa = 11.84 TVEE1024 pKa = 4.43 SNDD1027 pKa = 3.38 LYY1029 pKa = 11.6 ANTALKK1035 pKa = 10.25 LARR1038 pKa = 11.84 PSPGMVHH1045 pKa = 6.16 VPYY1048 pKa = 9.18 TQTPSGFKK1056 pKa = 10.14 YY1057 pKa = 9.05 WLKK1060 pKa = 11.03 EE1061 pKa = 3.53 KK1062 pKa = 9.68 GTALNTKK1069 pKa = 10.09 APFGCQIKK1077 pKa = 8.87 TNPVRR1082 pKa = 11.84 AMNCAVGNIPVSMNLPDD1099 pKa = 3.47 SAFTRR1104 pKa = 11.84 IVEE1107 pKa = 4.22 APTIIDD1113 pKa = 3.85 LTCTVATCTHH1123 pKa = 6.42 SSDD1126 pKa = 3.51 FGGVLTLTYY1135 pKa = 8.8 KK1136 pKa = 9.63 TNKK1139 pKa = 10.3 NGDD1142 pKa = 4.08 CSVHH1146 pKa = 5.16 SHH1148 pKa = 6.15 SNVATLQEE1156 pKa = 3.85 ATAKK1160 pKa = 10.48 VKK1162 pKa = 9.41 TAGKK1166 pKa = 8.12 VTLHH1170 pKa = 6.7 FSTASASPSFVVSLCSARR1188 pKa = 11.84 ATCSASCEE1196 pKa = 4.12 PPKK1199 pKa = 10.95 DD1200 pKa = 3.7 HH1201 pKa = 6.89 IVPYY1205 pKa = 10.09 AASHH1209 pKa = 6.14 SNVVFPDD1216 pKa = 3.3 MSGTALSWVQKK1227 pKa = 10.45 ISGGLGAFAIGAILVLVVVTCIGLRR1252 pKa = 11.84 RR1253 pKa = 3.66
Molecular weight: 138.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.777
IPC2_protein 7.936
IPC_protein 7.673
Toseland 7.263
ProMoST 8.346
Dawson 8.434
Bjellqvist 8.858
Wikipedia 8.346
Rodwell 8.478
Grimsley 7.322
Solomon 8.448
Lehninger 8.478
Nozaki 9.092
DTASelect 8.536
Thurlkill 8.609
EMBOSS 8.624
Sillero 8.946
Patrickios 3.935
IPC_peptide 8.448
IPC2_peptide 7.878
IPC2.peptide.svr19 8.029
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3685
1253
2432
1842.5
203.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.227 ± 0.215
2.985 ± 0.495
5.346 ± 0.607
5.075 ± 0.588
3.202 ± 0.053
5.726 ± 0.433
2.904 ± 0.356
4.288 ± 0.06
6.133 ± 0.147
7.924 ± 1.135
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.198 ± 0.021
3.474 ± 0.256
6.106 ± 0.584
3.229 ± 0.399
5.482 ± 0.64
6.214 ± 0.087
7.273 ± 0.275
8.521 ± 0.129
1.085 ± 0.159
3.609 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here