Bacillus subtilis (strain 168)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus subtilis group; Bacillus subtilis; Bacillus subtilis subsp. subtilis

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P94529|ARAP_BACSU Arabinooligosaccharides transport system permease protein AraP OS=Bacillus subtilis (strain 168) OX=224308 GN=araP PE=1 SV=1
MM1 pKa = 7.77EE2 pKa = 6.06DD3 pKa = 3.04ATFHH7 pKa = 6.49FKK9 pKa = 10.96DD10 pKa = 3.35PASPQEE16 pKa = 3.91ISDD19 pKa = 3.81IEE21 pKa = 4.23QKK23 pKa = 10.99LGVTFPNDD31 pKa = 3.24YY32 pKa = 11.09KK33 pKa = 11.26EE34 pKa = 5.61FLLQHH39 pKa = 6.47NGMEE43 pKa = 4.14MFDD46 pKa = 4.21GIEE49 pKa = 3.91ILSLEE54 pKa = 4.54GIIEE58 pKa = 4.18YY59 pKa = 11.06NEE61 pKa = 3.95VQDD64 pKa = 4.29FPEE67 pKa = 5.74GYY69 pKa = 10.64VLIGYY74 pKa = 8.93HH75 pKa = 6.12FDD77 pKa = 2.93GRR79 pKa = 11.84YY80 pKa = 10.3VIDD83 pKa = 3.62TNKK86 pKa = 10.49SKK88 pKa = 11.31NGLGYY93 pKa = 9.49MLYY96 pKa = 10.33LDD98 pKa = 5.3SIDD101 pKa = 5.55DD102 pKa = 3.85IEE104 pKa = 5.03DD105 pKa = 4.67AINLDD110 pKa = 4.0SNFEE114 pKa = 3.71IWFDD118 pKa = 3.57MLVSLNGTKK127 pKa = 10.17YY128 pKa = 9.76WEE130 pKa = 4.26VNPNLQEE137 pKa = 4.05YY138 pKa = 9.06YY139 pKa = 10.91KK140 pKa = 10.7LVSEE144 pKa = 4.68

Molecular weight: 16.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P06628|SP0F_BACSU Sporulation initiation phosphotransferase F OS=Bacillus subtilis (strain 168) OX=224308 GN=spo0F PE=1 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.27RR12 pKa = 11.84SKK14 pKa = 9.34VHH16 pKa = 5.82GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.55NGRR28 pKa = 11.84LVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4260

7

4267

1236478

20

5488

289.8

32.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.681 ± 0.04

0.787 ± 0.011

5.185 ± 0.037

7.26 ± 0.058

4.5 ± 0.033

6.912 ± 0.039

2.268 ± 0.017

7.371 ± 0.038

7.071 ± 0.044

9.657 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.789 ± 0.018

3.954 ± 0.029

3.664 ± 0.024

3.835 ± 0.027

4.092 ± 0.03

6.278 ± 0.034

5.422 ± 0.023

6.751 ± 0.03

1.033 ± 0.013

3.49 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski