Halogeometricum pleomorphic virus 1
Average proteome isoelectric point is 5.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H9ABQ4|H9ABQ4_9VIRU VP3 OS=Halogeometricum pleomorphic virus 1 OX=1156722 GN=3 PE=4 SV=1
MM1 pKa = 7.61 SKK3 pKa = 7.89 MTIDD7 pKa = 4.11 PEE9 pKa = 4.61 DD10 pKa = 3.49 VASTVATFIGTSSMAGIAQWSLFDD34 pKa = 4.95 VSLSDD39 pKa = 4.72 QAFQLAGNSVTLATVLTAVALAAIVFTNDD68 pKa = 2.71 NGSIDD73 pKa = 3.94 EE74 pKa = 4.4 LHH76 pKa = 6.97 AKK78 pKa = 9.53 AKK80 pKa = 10.7 KK81 pKa = 9.83 LDD83 pKa = 3.16 NYY85 pKa = 10.25 YY86 pKa = 10.11 YY87 pKa = 9.57 YY88 pKa = 10.93 SIVGSVALLVGWVFVGDD105 pKa = 3.46 VSSFITSSDD114 pKa = 3.03 LWGVGYY120 pKa = 10.49 VAVSMVAHH128 pKa = 6.69 FALGWMLL135 pKa = 3.96
Molecular weight: 14.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.843
IPC2_protein 4.126
IPC_protein 4.05
Toseland 3.834
ProMoST 4.253
Dawson 4.062
Bjellqvist 4.228
Wikipedia 4.05
Rodwell 3.884
Grimsley 3.745
Solomon 4.05
Lehninger 4.012
Nozaki 4.202
DTASelect 4.482
Thurlkill 3.91
EMBOSS 4.062
Sillero 4.177
Patrickios 1.977
IPC_peptide 4.05
IPC2_peptide 4.151
IPC2.peptide.svr19 4.066
Protein with the highest isoelectric point:
>tr|H9ABR1|H9ABR1_9VIRU ORF10 OS=Halogeometricum pleomorphic virus 1 OX=1156722 GN=ORF10 PE=4 SV=1
MM1 pKa = 7.67 AKK3 pKa = 9.91 NRR5 pKa = 11.84 SKK7 pKa = 10.76 KK8 pKa = 9.7 AARR11 pKa = 11.84 FGAFAEE17 pKa = 4.29 RR18 pKa = 11.84 AAFGRR23 pKa = 11.84 YY24 pKa = 8.5 GLEE27 pKa = 3.93 PDD29 pKa = 3.3 RR30 pKa = 11.84 DD31 pKa = 3.73 SWHH34 pKa = 7.44 DD35 pKa = 3.25 ARR37 pKa = 11.84 DD38 pKa = 3.53 RR39 pKa = 11.84 DD40 pKa = 3.8 GRR42 pKa = 11.84 PWDD45 pKa = 3.78 VKK47 pKa = 11.23 ACMLSRR53 pKa = 11.84 DD54 pKa = 3.62 SARR57 pKa = 11.84 FRR59 pKa = 11.84 LWADD63 pKa = 2.78 QHH65 pKa = 6.27 SRR67 pKa = 11.84 LRR69 pKa = 11.84 SEE71 pKa = 3.83 GGGYY75 pKa = 10.22 VFVGYY80 pKa = 10.11 LPRR83 pKa = 11.84 GSGIQVKK90 pKa = 7.51 QMRR93 pKa = 11.84 TVRR96 pKa = 11.84 AASITAEE103 pKa = 4.43 FYY105 pKa = 9.92 TAGNHH110 pKa = 6.63 AKK112 pKa = 10.26 GDD114 pKa = 3.7 QLKK117 pKa = 9.74 IPPRR121 pKa = 11.84 AVFVRR126 pKa = 3.95
Molecular weight: 14.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.765
IPC_protein 10.76
Toseland 10.847
ProMoST 10.965
Dawson 10.935
Bjellqvist 10.701
Wikipedia 11.199
Rodwell 11.038
Grimsley 10.979
Solomon 11.125
Lehninger 11.082
Nozaki 10.833
DTASelect 10.701
Thurlkill 10.847
EMBOSS 11.272
Sillero 10.877
Patrickios 10.76
IPC_peptide 11.125
IPC2_peptide 9.75
IPC2.peptide.svr19 8.716
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
2950
58
544
196.7
21.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.102 ± 0.714
0.678 ± 0.207
7.729 ± 0.633
7.254 ± 0.589
3.288 ± 0.351
8.644 ± 0.448
2.034 ± 0.288
3.593 ± 0.275
3.322 ± 0.378
7.356 ± 0.39
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.203 ± 0.263
3.627 ± 0.878
3.695 ± 0.573
3.051 ± 0.229
6.102 ± 0.876
7.593 ± 0.729
6.949 ± 1.174
7.831 ± 0.629
1.695 ± 0.203
3.254 ± 0.431
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here