Taterapox virus
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 220 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q0NPH1|Q0NPH1_9POXV Uncharacterized protein OS=Taterapox virus OX=28871 GN=TATV_DAH68_040 PE=4 SV=1
MM1 pKa = 7.92 DD2 pKa = 4.65 NSMLPMFMCNNIVDD16 pKa = 4.18 YY17 pKa = 11.36 VDD19 pKa = 5.92 DD20 pKa = 6.29 DD21 pKa = 4.34 IDD23 pKa = 6.64 DD24 pKa = 4.25 IDD26 pKa = 6.78 DD27 pKa = 4.29 IDD29 pKa = 6.8 DD30 pKa = 4.29 IDD32 pKa = 6.8 DD33 pKa = 4.29 IDD35 pKa = 6.8 DD36 pKa = 4.29 IDD38 pKa = 6.8 DD39 pKa = 4.29 IDD41 pKa = 6.8 DD42 pKa = 4.29 IDD44 pKa = 6.8 DD45 pKa = 4.29 IDD47 pKa = 6.8 DD48 pKa = 4.29 IDD50 pKa = 6.8 DD51 pKa = 4.29 IDD53 pKa = 6.8 DD54 pKa = 4.29 IDD56 pKa = 6.8 DD57 pKa = 4.29 IDD59 pKa = 6.8 DD60 pKa = 4.29 IDD62 pKa = 6.8 DD63 pKa = 4.29 IDD65 pKa = 6.8 DD66 pKa = 4.29 IDD68 pKa = 6.8 DD69 pKa = 4.29 IDD71 pKa = 6.8 DD72 pKa = 4.29 IDD74 pKa = 6.8 DD75 pKa = 4.29 IDD77 pKa = 6.8 DD78 pKa = 4.29 IDD80 pKa = 6.8 DD81 pKa = 4.29 IDD83 pKa = 6.8 DD84 pKa = 4.29 IDD86 pKa = 6.8 DD87 pKa = 4.29 IDD89 pKa = 6.8 DD90 pKa = 4.29 IDD92 pKa = 6.8 DD93 pKa = 4.29 IDD95 pKa = 6.97 DD96 pKa = 4.83 IDD98 pKa = 6.85 DD99 pKa = 3.74 IDD101 pKa = 4.28 NDD103 pKa = 3.53 IVQDD107 pKa = 3.91 IEE109 pKa = 5.67 DD110 pKa = 3.87 EE111 pKa = 4.28 ASNNDD116 pKa = 3.26 DD117 pKa = 3.51 HH118 pKa = 8.36 NYY120 pKa = 10.23 VYY122 pKa = 10.27 PLPEE126 pKa = 3.83 NMVYY130 pKa = 10.53 RR131 pKa = 11.84 FDD133 pKa = 3.76 KK134 pKa = 10.0 STNILDD140 pKa = 3.9 YY141 pKa = 11.21 LSTEE145 pKa = 4.1 RR146 pKa = 11.84 DD147 pKa = 3.57 HH148 pKa = 10.24 VMMAVQYY155 pKa = 10.31 YY156 pKa = 8.63 MSKK159 pKa = 10.26 QRR161 pKa = 11.84 LDD163 pKa = 3.35 DD164 pKa = 4.85 LYY166 pKa = 11.09 RR167 pKa = 11.84 QLPTKK172 pKa = 8.67 TRR174 pKa = 11.84 SYY176 pKa = 10.88 IDD178 pKa = 4.43 IINMYY183 pKa = 9.31 CDD185 pKa = 3.99 KK186 pKa = 11.33 VNNDD190 pKa = 3.6 YY191 pKa = 10.97 NRR193 pKa = 11.84 EE194 pKa = 3.94 MNIMYY199 pKa = 10.7 DD200 pKa = 3.47 MASTEE205 pKa = 4.21 SFTVYY210 pKa = 10.63 DD211 pKa = 3.93 INNEE215 pKa = 3.95 VNTILMDD222 pKa = 3.83 NKK224 pKa = 10.79 GLGVRR229 pKa = 11.84 LATISFITEE238 pKa = 4.16 LGRR241 pKa = 11.84 RR242 pKa = 11.84 CMNPVEE248 pKa = 4.32 TIKK251 pKa = 10.52 MFTLLSHH258 pKa = 6.92 TICDD262 pKa = 3.94 DD263 pKa = 3.66 CFVDD267 pKa = 4.46 YY268 pKa = 9.54 ITDD271 pKa = 3.95 ISPQDD276 pKa = 3.29 NTIPNTSTRR285 pKa = 11.84 EE286 pKa = 3.86 YY287 pKa = 10.49 LKK289 pKa = 10.99 LIGITAIMFATYY301 pKa = 7.95 KK302 pKa = 8.27 TLKK305 pKa = 10.56 YY306 pKa = 10.15 MIGG309 pKa = 3.33
Molecular weight: 36.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.818
IPC2_protein 3.363
IPC_protein 3.439
Toseland 3.185
ProMoST 3.63
Dawson 3.465
Bjellqvist 3.617
Wikipedia 3.452
Rodwell 3.249
Grimsley 3.083
Solomon 3.452
Lehninger 3.414
Nozaki 3.567
DTASelect 3.923
Thurlkill 3.249
EMBOSS 3.452
Sillero 3.567
Patrickios 1.049
IPC_peptide 3.439
IPC2_peptide 3.528
IPC2.peptide.svr19 3.638
Protein with the highest isoelectric point:
>tr|Q0NP56|Q0NP56_9POXV Uncharacterized protein OS=Taterapox virus OX=28871 GN=TATV_DAH68_155 PE=4 SV=1
MM1 pKa = 7.68 KK2 pKa = 10.3 SLNRR6 pKa = 11.84 QTVSRR11 pKa = 11.84 FKK13 pKa = 10.83 KK14 pKa = 10.2 LSVPAAIMMILSTIISGIGTFLHH37 pKa = 6.2 YY38 pKa = 10.29 KK39 pKa = 9.66 EE40 pKa = 5.07 EE41 pKa = 4.23 LMPSACANGWIQYY54 pKa = 9.45 DD55 pKa = 3.41 KK56 pKa = 11.16 HH57 pKa = 7.66 CYY59 pKa = 10.14 LDD61 pKa = 3.76 TNIKK65 pKa = 9.77 MSTDD69 pKa = 2.97 NAVYY73 pKa = 9.87 QCRR76 pKa = 11.84 KK77 pKa = 9.5 LRR79 pKa = 11.84 ARR81 pKa = 11.84 LPRR84 pKa = 11.84 PDD86 pKa = 3.46 TRR88 pKa = 11.84 HH89 pKa = 5.59 LRR91 pKa = 11.84 VLFSIFYY98 pKa = 9.15 KK99 pKa = 10.52 DD100 pKa = 3.24 YY101 pKa = 9.86 WVSLKK106 pKa = 9.25 KK107 pKa = 9.24 TNNKK111 pKa = 8.56 WLDD114 pKa = 3.23 INNDD118 pKa = 3.01 KK119 pKa = 11.26 DD120 pKa = 3.45 IDD122 pKa = 3.75 ISKK125 pKa = 8.87 LTNFKK130 pKa = 10.39 QLNSTTDD137 pKa = 3.03 AEE139 pKa = 3.84 ACYY142 pKa = 9.72 IYY144 pKa = 10.87 KK145 pKa = 10.25 SGKK148 pKa = 8.99 LVKK151 pKa = 8.76 TVCKK155 pKa = 9.32 STQSVLCVKK164 pKa = 10.35 RR165 pKa = 11.84 FYY167 pKa = 10.92 KK168 pKa = 10.71
Molecular weight: 19.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.104
IPC2_protein 9.121
IPC_protein 9.019
Toseland 9.794
ProMoST 9.531
Dawson 10.014
Bjellqvist 9.721
Wikipedia 10.175
Rodwell 10.482
Grimsley 10.087
Solomon 10.043
Lehninger 10.014
Nozaki 9.897
DTASelect 9.677
Thurlkill 9.867
EMBOSS 10.204
Sillero 9.955
Patrickios 9.736
IPC_peptide 10.043
IPC2_peptide 8.448
IPC2.peptide.svr19 8.01
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
220
0
220
58050
47
1913
263.9
30.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.802 ± 0.12
2.114 ± 0.084
6.815 ± 0.172
5.433 ± 0.147
4.506 ± 0.118
3.974 ± 0.114
2.124 ± 0.089
9.587 ± 0.181
7.239 ± 0.109
8.798 ± 0.132
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.848 ± 0.084
6.973 ± 0.121
3.318 ± 0.114
2.226 ± 0.086
4.289 ± 0.121
7.817 ± 0.15
6.045 ± 0.117
6.062 ± 0.106
0.708 ± 0.047
5.323 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here