Streptomyces phage SF1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1Y5S2|A0A0K1Y5S2_9CAUD Uncharacterized protein OS=Streptomyces phage SF1 OX=1690817 GN=SF1_210 PE=4 SV=1
MM1 pKa = 7.84AFPNPNLLPDD11 pKa = 4.53DD12 pKa = 4.66ASQFEE17 pKa = 4.85GATHH21 pKa = 6.26SWIDD25 pKa = 3.47PSNATLSIVSGQYY38 pKa = 8.58MAGSKK43 pKa = 8.77SLRR46 pKa = 11.84LTATAAGTASASTPYY61 pKa = 10.29VLSGLAPGKK70 pKa = 9.17TYY72 pKa = 10.44VARR75 pKa = 11.84IPIRR79 pKa = 11.84VSTASAGKK87 pKa = 7.58TATVRR92 pKa = 11.84MAFYY96 pKa = 10.62ADD98 pKa = 3.8SGPSIGTMDD107 pKa = 3.57AVVNLSATGTGWLPDD122 pKa = 3.61NFPTVSMVAPPAAAKK137 pKa = 10.29VRR139 pKa = 11.84MVVTVTGMAAGEE151 pKa = 4.19YY152 pKa = 10.51VNFDD156 pKa = 3.36DD157 pKa = 6.11CYY159 pKa = 10.9LGEE162 pKa = 4.84APLIPGNLYY171 pKa = 9.97PYY173 pKa = 8.69TVQSMEE179 pKa = 4.58SGVSTWGATGATTLWGDD196 pKa = 3.69GTRR199 pKa = 11.84SDD201 pKa = 3.75GFRR204 pKa = 11.84NLGLTATSAGVIAARR219 pKa = 11.84PNTYY223 pKa = 10.28VPVSAGAEE231 pKa = 4.13YY232 pKa = 10.75VGEE235 pKa = 4.0AWVYY239 pKa = 9.87SAVATTVDD247 pKa = 4.53ALIVWADD254 pKa = 3.43STGTEE259 pKa = 3.94VGRR262 pKa = 11.84TLVTRR267 pKa = 11.84DD268 pKa = 3.37ATSGAWSYY276 pKa = 10.49FIAVGVAPPNATQARR291 pKa = 11.84LFIQPKK297 pKa = 10.02ALATGDD303 pKa = 3.47TFYY306 pKa = 11.56VDD308 pKa = 3.51EE309 pKa = 5.1AALKK313 pKa = 9.11TSPNPTTNLLTYY325 pKa = 10.38DD326 pKa = 3.92EE327 pKa = 5.13YY328 pKa = 11.05STEE331 pKa = 4.08STLPPWTWDD340 pKa = 2.84GGVADD345 pKa = 4.74RR346 pKa = 11.84SYY348 pKa = 10.94FYY350 pKa = 10.7STITDD355 pKa = 2.75GRR357 pKa = 11.84YY358 pKa = 8.16IQRR361 pKa = 11.84VTPDD365 pKa = 3.07GPGYY369 pKa = 10.79LSGFMDD375 pKa = 3.76RR376 pKa = 11.84LVPVTEE382 pKa = 3.96GTTYY386 pKa = 10.45QLRR389 pKa = 11.84TLVLRR394 pKa = 11.84RR395 pKa = 11.84NPTAEE400 pKa = 4.85AISLTARR407 pKa = 11.84VRR409 pKa = 11.84IDD411 pKa = 2.87WYY413 pKa = 10.57DD414 pKa = 3.65DD415 pKa = 3.18NGTMVQADD423 pKa = 4.01VPDD426 pKa = 3.64QYY428 pKa = 11.77ATVTEE433 pKa = 4.38SVTYY437 pKa = 10.47SGVLLGEE444 pKa = 4.39TRR446 pKa = 11.84TCPPGATRR454 pKa = 11.84ARR456 pKa = 11.84LGVDD460 pKa = 2.69IDD462 pKa = 3.79HH463 pKa = 6.61TQTLAEE469 pKa = 4.77FYY471 pKa = 10.78YY472 pKa = 10.31VDD474 pKa = 3.41KK475 pKa = 10.99FQFYY479 pKa = 11.08VSDD482 pKa = 3.27SLYY485 pKa = 10.94AVTVSDD491 pKa = 3.63SRR493 pKa = 11.84GSIALQVNYY502 pKa = 10.2SPAGATSVSIRR513 pKa = 11.84RR514 pKa = 11.84MDD516 pKa = 3.64EE517 pKa = 4.53DD518 pKa = 4.08GSSTYY523 pKa = 10.2IRR525 pKa = 11.84GYY527 pKa = 9.17GQEE530 pKa = 4.16FNKK533 pKa = 10.39APYY536 pKa = 10.12SPGPIVAEE544 pKa = 4.71DD545 pKa = 3.72YY546 pKa = 7.97EE547 pKa = 5.6APLGTRR553 pKa = 11.84VWYY556 pKa = 10.17ALEE559 pKa = 3.94WFGASGSPVARR570 pKa = 11.84VFTQTVTGPVLEE582 pKa = 5.43DD583 pKa = 3.32GDD585 pKa = 4.53YY586 pKa = 11.52VWFKK590 pKa = 11.52SPGLPALNVQVMMEE604 pKa = 4.09TAIKK608 pKa = 9.47WEE610 pKa = 3.75RR611 pKa = 11.84AARR614 pKa = 11.84KK615 pKa = 7.64TSLTIVGRR623 pKa = 11.84KK624 pKa = 9.18NPVDD628 pKa = 3.18ITDD631 pKa = 3.31VRR633 pKa = 11.84GGRR636 pKa = 11.84TGSLSILVWDD646 pKa = 4.77EE647 pKa = 4.01SSHH650 pKa = 6.09VLFDD654 pKa = 3.57QLLDD658 pKa = 3.52SGLPVLIQAMPGYY671 pKa = 9.99GVAGNLFLSIGDD683 pKa = 3.53VSAEE687 pKa = 3.98NVLGVANEE695 pKa = 4.21PGWRR699 pKa = 11.84WALAVSEE706 pKa = 4.47IDD708 pKa = 3.84RR709 pKa = 11.84PDD711 pKa = 3.74GGLQGSAGRR720 pKa = 11.84TWQDD724 pKa = 2.98VMDD727 pKa = 5.39EE728 pKa = 4.23NADD731 pKa = 3.45WAAVDD736 pKa = 4.36AKK738 pKa = 9.91FATWAGVLTNNTT750 pKa = 3.56

Molecular weight: 80.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1Y5T7|A0A0K1Y5T7_9CAUD Uncharacterized protein OS=Streptomyces phage SF1 OX=1690817 GN=SF1_360 PE=4 SV=1
MM1 pKa = 7.11TMARR5 pKa = 11.84PSFRR9 pKa = 11.84PNRR12 pKa = 11.84RR13 pKa = 11.84NIAGFLKK20 pKa = 10.16TPATHH25 pKa = 7.25ALIEE29 pKa = 4.43RR30 pKa = 11.84KK31 pKa = 7.64TRR33 pKa = 11.84AAEE36 pKa = 4.14SAASAAARR44 pKa = 11.84SDD46 pKa = 2.9GWGGQFRR53 pKa = 11.84TDD55 pKa = 3.23VEE57 pKa = 4.57TGDD60 pKa = 3.2KK61 pKa = 9.89RR62 pKa = 11.84ARR64 pKa = 11.84GAVIGDD70 pKa = 3.53YY71 pKa = 8.43STPYY75 pKa = 9.98PEE77 pKa = 4.13VSRR80 pKa = 11.84RR81 pKa = 11.84ALLRR85 pKa = 11.84ALDD88 pKa = 3.81AARR91 pKa = 11.84GAEE94 pKa = 3.93

Molecular weight: 10.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

13540

32

1190

260.4

27.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.888 ± 0.447

0.598 ± 0.104

6.551 ± 0.302

5.244 ± 0.422

2.386 ± 0.187

10.074 ± 0.513

1.396 ± 0.2

3.538 ± 0.143

3.405 ± 0.383

8.235 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.92 ± 0.136

2.563 ± 0.191

5.643 ± 0.325

3.124 ± 0.161

7.186 ± 0.433

5.465 ± 0.247

6.78 ± 0.415

8.227 ± 0.282

2.157 ± 0.149

2.622 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski