Shuangao insect virus 7
Average proteome isoelectric point is 7.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P0QL37|A0A0P0QL37_9VIRU Putative glycoprotein OS=Shuangao insect virus 7 OX=1746063 GN=VP1 PE=4 SV=1
MM1 pKa = 7.96 DD2 pKa = 4.91 SEE4 pKa = 4.44 TSYY7 pKa = 11.6 VEE9 pKa = 4.45 GWKK12 pKa = 10.38 LWGCQSFRR20 pKa = 11.84 FGFYY24 pKa = 10.27 VRR26 pKa = 11.84 QTDD29 pKa = 3.59 DD30 pKa = 4.27 GEE32 pKa = 4.34 PSAATEE38 pKa = 3.93 PPIQEE43 pKa = 4.33 RR44 pKa = 11.84 KK45 pKa = 10.23 LEE47 pKa = 4.18 EE48 pKa = 4.04 PWEE51 pKa = 4.17 VGVDD55 pKa = 3.4 RR56 pKa = 11.84 EE57 pKa = 4.23 EE58 pKa = 3.93 MEE60 pKa = 3.7 KK61 pKa = 10.55 ARR63 pKa = 11.84 RR64 pKa = 11.84 IRR66 pKa = 11.84 EE67 pKa = 3.67 EE68 pKa = 3.95 AEE70 pKa = 4.0 AIEE73 pKa = 4.65 EE74 pKa = 3.95 KK75 pKa = 10.81 RR76 pKa = 11.84 KK77 pKa = 10.31 IKK79 pKa = 9.38 WSKK82 pKa = 10.74 DD83 pKa = 3.11 LNAVANFNKK92 pKa = 9.66 IWFPPLLEE100 pKa = 4.12 EE101 pKa = 5.55 PIVHH105 pKa = 6.15 VLRR108 pKa = 11.84 TKK110 pKa = 10.8 LPNLDD115 pKa = 3.53 RR116 pKa = 11.84 DD117 pKa = 3.97 YY118 pKa = 11.5 LAWTVLTAEE127 pKa = 4.15 VLLYY131 pKa = 11.24 VLMSYY136 pKa = 9.47 MVGKK140 pKa = 10.17 VSHH143 pKa = 5.84 VLVFIMHH150 pKa = 7.36 LGLSLYY156 pKa = 10.58 FEE158 pKa = 4.85 EE159 pKa = 5.49 NGVFMAFAAFWILNKK174 pKa = 9.95 ACRR177 pKa = 11.84 IFTRR181 pKa = 11.84 PKK183 pKa = 8.71 EE184 pKa = 3.88 YY185 pKa = 9.96 RR186 pKa = 11.84 IYY188 pKa = 11.07 EE189 pKa = 4.19 EE190 pKa = 4.08 LHH192 pKa = 5.83 YY193 pKa = 9.45 ATPRR197 pKa = 11.84 DD198 pKa = 3.62 GPFIKK203 pKa = 10.23 AYY205 pKa = 10.13 EE206 pKa = 4.0 PLIYY210 pKa = 10.42 LAVLLGTSYY219 pKa = 11.15 LLHH222 pKa = 5.81 GTSFGSTFGSVWILVVIIVMSKK244 pKa = 8.77 MVPGVGGNSTMGLMTFIVVLIIGIATVPEE273 pKa = 4.09 IWSSLILVTKK283 pKa = 10.39 RR284 pKa = 11.84 AIAPAEE290 pKa = 4.27 VASGPPKK297 pKa = 9.85 WYY299 pKa = 10.62 GGVASWATSLPVIGYY314 pKa = 8.86 SFNTLWDD321 pKa = 3.79 VLRR324 pKa = 11.84 KK325 pKa = 7.44 MCCYY329 pKa = 10.59 VPLIWIAIDD338 pKa = 4.39 DD339 pKa = 3.68 IRR341 pKa = 11.84 GPGLGISKK349 pKa = 10.04 AWEE352 pKa = 3.65 THH354 pKa = 4.92 AKK356 pKa = 9.7 KK357 pKa = 10.5 DD358 pKa = 3.48 SLLGHH363 pKa = 6.17 GTAALYY369 pKa = 10.31 KK370 pKa = 9.56 GAGWLPVVVEE380 pKa = 5.58 CVLLWWRR387 pKa = 11.84 GSMVEE392 pKa = 5.35 IVILWVVAVLTWLAWDD408 pKa = 3.42 VFGRR412 pKa = 11.84 NEE414 pKa = 4.1 WIGRR418 pKa = 11.84 GAGMTVTTVRR428 pKa = 11.84 PGWRR432 pKa = 11.84 TVFGHH437 pKa = 6.12 GPEE440 pKa = 4.18 GLRR443 pKa = 11.84 VMVFQISTLAAWLILFISTMSQVSMLGVAFLLTVLNKK480 pKa = 9.65 EE481 pKa = 3.82 RR482 pKa = 11.84 ALALMLGIGTLSPVWLWRR500 pKa = 11.84 AWKK503 pKa = 9.76 SDD505 pKa = 2.99 KK506 pKa = 11.2 PMTEE510 pKa = 4.02 SLNQNSVDD518 pKa = 3.39 AHH520 pKa = 6.32 PPGAEE525 pKa = 4.04 YY526 pKa = 10.35 IEE528 pKa = 4.31
Molecular weight: 59.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.155
IPC2_protein 6.173
IPC_protein 6.262
Toseland 6.547
ProMoST 6.453
Dawson 6.576
Bjellqvist 6.547
Wikipedia 6.576
Rodwell 6.561
Grimsley 6.678
Solomon 6.576
Lehninger 6.576
Nozaki 6.825
DTASelect 7.0
Thurlkill 7.029
EMBOSS 7.0
Sillero 6.956
Patrickios 4.266
IPC_peptide 6.59
IPC2_peptide 6.766
IPC2.peptide.svr19 6.796
Protein with the highest isoelectric point:
>tr|A0A0P0QKD5|A0A0P0QKD5_9VIRU Genome polyprotein OS=Shuangao insect virus 7 OX=1746063 PE=4 SV=1
MM1 pKa = 7.84 IIYY4 pKa = 9.87 IILVGIVGIILEE16 pKa = 4.15 ILDD19 pKa = 3.31 RR20 pKa = 11.84 RR21 pKa = 11.84 GANRR25 pKa = 11.84 EE26 pKa = 4.03 SPLEE30 pKa = 3.88 VLLRR34 pKa = 11.84 LSDD37 pKa = 3.72 IFAKK41 pKa = 10.51 PDD43 pKa = 3.07 VTRR46 pKa = 11.84 WGGEE50 pKa = 3.81 YY51 pKa = 10.47 KK52 pKa = 10.41 EE53 pKa = 4.18 ALRR56 pKa = 11.84 GLGARR61 pKa = 11.84 AFNAFKK67 pKa = 10.74 NRR69 pKa = 11.84 GVRR72 pKa = 11.84 RR73 pKa = 11.84 VDD75 pKa = 3.26 KK76 pKa = 10.41 DD77 pKa = 3.09 PHH79 pKa = 5.67 VASRR83 pKa = 11.84 GYY85 pKa = 11.01 DD86 pKa = 3.15 KK87 pKa = 11.13 LLEE90 pKa = 3.91 ISIAARR96 pKa = 11.84 VKK98 pKa = 10.33 PRR100 pKa = 11.84 GAVLSLCCGRR110 pKa = 11.84 GGWEE114 pKa = 3.68 QLYY117 pKa = 10.37 AASPDD122 pKa = 3.49 VTRR125 pKa = 11.84 ITAVTLGKK133 pKa = 10.91 GPGHH137 pKa = 6.64 EE138 pKa = 4.15 GHH140 pKa = 7.07 EE141 pKa = 4.66 DD142 pKa = 3.16 FTDD145 pKa = 3.27 NYY147 pKa = 10.22 FPGRR151 pKa = 11.84 EE152 pKa = 4.05 KK153 pKa = 10.88 VCLRR157 pKa = 11.84 FADD160 pKa = 3.66 ATSYY164 pKa = 10.79 PITAHH169 pKa = 6.43 DD170 pKa = 3.79 TLLFDD175 pKa = 5.08 GGEE178 pKa = 4.16 SHH180 pKa = 7.99 PDD182 pKa = 3.21 FSVEE186 pKa = 3.93 ANRR189 pKa = 11.84 FNQLFAGAVMRR200 pKa = 11.84 QITPNTKK207 pKa = 8.92 TFILKK212 pKa = 9.96 ILTPTSPEE220 pKa = 4.32 TIRR223 pKa = 11.84 MMQAIQTMTGRR234 pKa = 11.84 GSLYY238 pKa = 9.39 RR239 pKa = 11.84 ASVSRR244 pKa = 11.84 NSTLEE249 pKa = 3.91 LYY251 pKa = 10.08 FVSTAPTNIRR261 pKa = 11.84 NQIRR265 pKa = 11.84 EE266 pKa = 4.2 LMQDD270 pKa = 2.99 MARR273 pKa = 11.84 RR274 pKa = 11.84 GIEE277 pKa = 3.78 NRR279 pKa = 11.84 KK280 pKa = 9.81 LEE282 pKa = 3.99 ARR284 pKa = 11.84 RR285 pKa = 11.84 AGPQYY290 pKa = 10.69 LYY292 pKa = 10.97 ARR294 pKa = 11.84 EE295 pKa = 4.43 EE296 pKa = 4.01 IQEE299 pKa = 4.23 TCVPLLATPDD309 pKa = 3.62 MSDD312 pKa = 3.53 SIAGLGEE319 pKa = 3.93 PLPEE323 pKa = 3.66 VGRR326 pKa = 11.84 AYY328 pKa = 10.89 NHH330 pKa = 6.45 WEE332 pKa = 3.96 SLGVYY337 pKa = 9.75 PMGVSGSKK345 pKa = 9.85 GMKK348 pKa = 7.42 YY349 pKa = 10.52 NKK351 pKa = 10.06 YY352 pKa = 10.76 GMACATRR359 pKa = 11.84 MLSSIDD365 pKa = 5.81 GFDD368 pKa = 3.03 QWKK371 pKa = 8.6 LTDD374 pKa = 3.49 TTPEE378 pKa = 4.01 GFVSVFNTKK387 pKa = 8.64 IDD389 pKa = 3.6 KK390 pKa = 10.5 PPTEE394 pKa = 3.68 NHH396 pKa = 6.66 PYY398 pKa = 10.39 EE399 pKa = 4.46 KK400 pKa = 10.43 RR401 pKa = 11.84 LWAVYY406 pKa = 10.57 KK407 pKa = 10.85 GMSSHH412 pKa = 5.79 FQARR416 pKa = 11.84 GFRR419 pKa = 11.84 LRR421 pKa = 11.84 EE422 pKa = 3.68 LSYY425 pKa = 11.56 DD426 pKa = 3.88 EE427 pKa = 5.85 IEE429 pKa = 4.27 KK430 pKa = 10.42 QVNNAGAPGYY440 pKa = 10.45 ADD442 pKa = 3.07 MMEE445 pKa = 4.32 GVNDD449 pKa = 3.27 IKK451 pKa = 11.17 DD452 pKa = 3.65 YY453 pKa = 10.35 MSRR456 pKa = 11.84 PDD458 pKa = 3.02 WRR460 pKa = 11.84 KK461 pKa = 9.31 KK462 pKa = 9.09 VEE464 pKa = 4.11 KK465 pKa = 10.29 LRR467 pKa = 11.84 QALLSGRR474 pKa = 11.84 PKK476 pKa = 10.17 EE477 pKa = 4.97 AIFNTMGKK485 pKa = 9.24 RR486 pKa = 11.84 EE487 pKa = 4.04 KK488 pKa = 10.35 KK489 pKa = 10.01 KK490 pKa = 10.91 SGGKK494 pKa = 8.06 GSRR497 pKa = 11.84 MIAYY501 pKa = 8.27 MSIGMRR507 pKa = 11.84 LLEE510 pKa = 4.12 MKK512 pKa = 10.84 LFGNLMKK519 pKa = 9.84 LTKK522 pKa = 9.67 PEE524 pKa = 3.91 YY525 pKa = 8.48 MHH527 pKa = 6.79 FGVGGFGLHH536 pKa = 7.23 DD537 pKa = 4.33 LGEE540 pKa = 4.14 RR541 pKa = 11.84 LRR543 pKa = 11.84 RR544 pKa = 11.84 VWKK547 pKa = 10.95 GEE549 pKa = 3.7 GSSDD553 pKa = 4.6 DD554 pKa = 3.16 IAGFDD559 pKa = 3.57 TRR561 pKa = 11.84 VSAKK565 pKa = 9.37 TLAFEE570 pKa = 4.08 AAFVQEE576 pKa = 4.31 LGGNTGHH583 pKa = 7.02 RR584 pKa = 11.84 EE585 pKa = 3.89 MYY587 pKa = 9.47 EE588 pKa = 4.5 VYY590 pKa = 10.32 ASPHH594 pKa = 5.53 ILIPVPSPHH603 pKa = 6.82 RR604 pKa = 11.84 RR605 pKa = 11.84 VQLLVGRR612 pKa = 11.84 GQRR615 pKa = 11.84 MSGCQVTYY623 pKa = 10.75 PMNTLTRR630 pKa = 11.84 VALFLVQASLAEE642 pKa = 4.39 GVDD645 pKa = 3.87 EE646 pKa = 4.89 NLVQAWVMRR655 pKa = 11.84 VMRR658 pKa = 11.84 GKK660 pKa = 10.47 EE661 pKa = 4.02 DD662 pKa = 2.7 IGGAVSGDD670 pKa = 3.33 DD671 pKa = 4.46 AVFTSSKK678 pKa = 10.17 KK679 pKa = 8.42 MKK681 pKa = 10.22 EE682 pKa = 4.28 IINHH686 pKa = 5.96 SDD688 pKa = 3.06 VLEE691 pKa = 4.59 DD692 pKa = 3.08 MGFPRR697 pKa = 11.84 KK698 pKa = 10.03 NMARR702 pKa = 11.84 GVRR705 pKa = 11.84 SPLMHH710 pKa = 7.37 DD711 pKa = 3.17 VRR713 pKa = 11.84 EE714 pKa = 4.23 VEE716 pKa = 4.7 FCSHH720 pKa = 6.96 RR721 pKa = 11.84 YY722 pKa = 9.28 EE723 pKa = 4.54 PVTFMDD729 pKa = 3.51 THH731 pKa = 6.02 TGKK734 pKa = 10.01 IVTKK738 pKa = 9.95 YY739 pKa = 9.88 MPTRR743 pKa = 11.84 DD744 pKa = 3.72 FGEE747 pKa = 4.1 IAAKK751 pKa = 10.41 SLIRR755 pKa = 11.84 TGGDD759 pKa = 3.14 VSDD762 pKa = 5.23 LDD764 pKa = 3.69 ALAWISAQGNNLLVNYY780 pKa = 7.9 PHH782 pKa = 6.92 LRR784 pKa = 11.84 SARR787 pKa = 11.84 ALGFAFKK794 pKa = 10.65 SVAPPNLVPDD804 pKa = 3.81 NKK806 pKa = 10.63 GGFMRR811 pKa = 11.84 PRR813 pKa = 11.84 PWMQPGDD820 pKa = 3.64 ILTVFNDD827 pKa = 3.2 VHH829 pKa = 6.75 FGNSSKK835 pKa = 11.05 YY836 pKa = 8.83 PIPGFEE842 pKa = 3.55 VRR844 pKa = 11.84 AWRR847 pKa = 11.84 NVGYY851 pKa = 9.94 LKK853 pKa = 10.68 PKK855 pKa = 10.28 IEE857 pKa = 4.2 TLYY860 pKa = 10.79 DD861 pKa = 3.62 PPSFTRR867 pKa = 11.84 RR868 pKa = 11.84 RR869 pKa = 11.84 SIWRR873 pKa = 11.84 DD874 pKa = 2.98 NLHH877 pKa = 7.01 RR878 pKa = 11.84 DD879 pKa = 3.4 VTKK882 pKa = 10.73 AIYY885 pKa = 10.52 DD886 pKa = 3.98 FGTGGNLIPLDD897 pKa = 3.58 LWRR900 pKa = 11.84 EE901 pKa = 3.79 RR902 pKa = 11.84 RR903 pKa = 11.84 LDD905 pKa = 3.33
Molecular weight: 101.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.823
IPC2_protein 8.799
IPC_protein 8.726
Toseland 9.37
ProMoST 9.209
Dawson 9.677
Bjellqvist 9.428
Wikipedia 9.867
Rodwell 9.882
Grimsley 9.765
Solomon 9.706
Lehninger 9.648
Nozaki 9.472
DTASelect 9.385
Thurlkill 9.516
EMBOSS 9.823
Sillero 9.619
Patrickios 4.978
IPC_peptide 9.692
IPC2_peptide 8.024
IPC2.peptide.svr19 7.768
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2996
136
905
499.3
56.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.308 ± 0.566
1.368 ± 0.38
4.64 ± 0.47
5.741 ± 0.324
3.772 ± 0.239
9.012 ± 0.354
2.336 ± 0.207
6.275 ± 0.461
5.107 ± 0.336
9.446 ± 0.449
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.672 ± 0.421
3.605 ± 0.278
4.439 ± 0.408
2.069 ± 0.382
6.208 ± 0.852
5.908 ± 0.386
5.941 ± 0.437
7.844 ± 0.716
2.47 ± 0.567
3.838 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here