Absiella sp. AM09-45

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Absiella; unclassified Absiella

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4075 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A372SIZ3|A0A372SIZ3_9FIRM Uncharacterized protein (Fragment) OS=Absiella sp. AM09-45 OX=2291993 GN=DW113_19505 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.31HH3 pKa = 5.71TEE5 pKa = 4.43HH6 pKa = 7.25KK7 pKa = 10.79SEE9 pKa = 4.53TIDD12 pKa = 3.81GFQYY16 pKa = 11.38DD17 pKa = 4.62NDD19 pKa = 3.97SQLYY23 pKa = 9.41KK24 pKa = 10.44ATIQNIDD31 pKa = 3.33FMMDD35 pKa = 2.94EE36 pKa = 4.33YY37 pKa = 11.06TDD39 pKa = 3.81EE40 pKa = 4.17NAEE43 pKa = 4.1YY44 pKa = 11.25AMKK47 pKa = 10.41LASAYY52 pKa = 10.09HH53 pKa = 6.73LYY55 pKa = 10.44LSKK58 pKa = 10.93IMDD61 pKa = 3.98FLLEE65 pKa = 4.38DD66 pKa = 3.34EE67 pKa = 5.01AFRR70 pKa = 11.84DD71 pKa = 3.98DD72 pKa = 6.62GIFPDD77 pKa = 3.91QNKK80 pKa = 7.39EE81 pKa = 4.13TLLPALGTPSILLLSNNDD99 pKa = 3.86GQCSYY104 pKa = 11.48CNQTLDD110 pKa = 3.6DD111 pKa = 3.54THH113 pKa = 8.03IISFEE118 pKa = 3.85FSDD121 pKa = 5.13IFDD124 pKa = 4.62DD125 pKa = 6.35LYY127 pKa = 11.63DD128 pKa = 3.75LTIEE132 pKa = 4.32GG133 pKa = 4.04

Molecular weight: 15.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A372SSQ4|A0A372SSQ4_9FIRM FMN-binding protein OS=Absiella sp. AM09-45 OX=2291993 GN=DW113_10765 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 10.05RR10 pKa = 11.84KK11 pKa = 8.47HH12 pKa = 5.31QKK14 pKa = 6.82THH16 pKa = 4.76GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 8.95VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.59GRR39 pKa = 11.84KK40 pKa = 8.44VLSAA44 pKa = 4.05

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4075

0

4075

1244012

22

3940

305.3

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.299 ± 0.035

1.52 ± 0.017

6.076 ± 0.035

7.012 ± 0.042

4.48 ± 0.03

5.877 ± 0.042

2.321 ± 0.023

8.619 ± 0.043

7.842 ± 0.035

9.179 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.448 ± 0.026

4.868 ± 0.035

2.926 ± 0.021

3.681 ± 0.026

3.418 ± 0.026

5.684 ± 0.028

5.094 ± 0.043

6.209 ± 0.031

0.829 ± 0.013

4.619 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski