Streptococcus satellite phage Javan154

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZFJ5|A0A4D5ZFJ5_9VIRU Integrase OS=Streptococcus satellite phage Javan154 OX=2558546 GN=JavanS154_0001 PE=3 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84TFSDD6 pKa = 3.67TPKK9 pKa = 10.21TFTFHH14 pKa = 5.7YY15 pKa = 8.55TFKK18 pKa = 11.1DD19 pKa = 3.09FDD21 pKa = 4.09TAQVACHH28 pKa = 6.88AILGYY33 pKa = 7.77MTGTYY38 pKa = 7.11EE39 pKa = 4.76QPVIDD44 pKa = 3.97ATYY47 pKa = 10.82HH48 pKa = 6.27NDD50 pKa = 3.67DD51 pKa = 3.19QGGYY55 pKa = 10.61ANQLVLEE62 pKa = 4.22YY63 pKa = 11.33AEE65 pKa = 4.34DD66 pKa = 3.58RR67 pKa = 11.84KK68 pKa = 10.83LNKK71 pKa = 9.29VFKK74 pKa = 10.18RR75 pKa = 11.84ICDD78 pKa = 3.69SFKK81 pKa = 10.93DD82 pKa = 4.21YY83 pKa = 11.52YY84 pKa = 10.51NQPEE88 pKa = 4.31DD89 pKa = 3.62MTDD92 pKa = 3.38EE93 pKa = 4.34EE94 pKa = 5.88LDD96 pKa = 3.72DD97 pKa = 3.78QRR99 pKa = 11.84VVSLSKK105 pKa = 9.84STQEE109 pKa = 4.12KK110 pKa = 10.62VNDD113 pKa = 4.0RR114 pKa = 11.84DD115 pKa = 3.47TFMTFISDD123 pKa = 3.61HH124 pKa = 5.84NQLAEE129 pKa = 4.25HH130 pKa = 6.91LSMNYY135 pKa = 10.19KK136 pKa = 10.52EE137 pKa = 4.5MTPEE141 pKa = 3.92DD142 pKa = 3.6LGAILEE148 pKa = 4.71SISQAFNHH156 pKa = 6.73LYY158 pKa = 11.22DD159 pKa = 3.83MVVEE163 pKa = 4.32GQLLVKK169 pKa = 10.69

Molecular weight: 19.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZFK5|A0A4D5ZFK5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan154 OX=2558546 GN=JavanS154_0011 PE=4 SV=1
MM1 pKa = 7.31IMKK4 pKa = 8.92ITEE7 pKa = 4.11HH8 pKa = 6.3KK9 pKa = 9.71KK10 pKa = 9.75KK11 pKa = 10.59NGTIVYY17 pKa = 9.02RR18 pKa = 11.84ASIYY22 pKa = 10.8LGIDD26 pKa = 2.83QMTGKK31 pKa = 10.05RR32 pKa = 11.84VKK34 pKa = 9.97TSITGRR40 pKa = 11.84TRR42 pKa = 11.84KK43 pKa = 9.11EE44 pKa = 3.7VNQKK48 pKa = 10.29AKK50 pKa = 10.21HH51 pKa = 5.3AQFDD55 pKa = 4.08FLSNGSTIHH64 pKa = 7.08KK65 pKa = 7.74EE66 pKa = 3.81AQIRR70 pKa = 11.84NYY72 pKa = 10.35QEE74 pKa = 5.23LAEE77 pKa = 4.65LWLKK81 pKa = 10.76SYY83 pKa = 11.01QLTVKK88 pKa = 9.5PQTYY92 pKa = 10.31EE93 pKa = 3.93STKK96 pKa = 10.89LLLKK100 pKa = 10.55NHH102 pKa = 7.01ILPVFGNMKK111 pKa = 10.26LEE113 pKa = 4.9RR114 pKa = 11.84ITPSFVQQFANKK126 pKa = 9.47LAHH129 pKa = 5.94TLVNFKK135 pKa = 9.7VACSINRR142 pKa = 11.84RR143 pKa = 11.84ILQYY147 pKa = 11.24AVLLQLIPYY156 pKa = 8.96NPARR160 pKa = 11.84EE161 pKa = 3.87IIIPKK166 pKa = 8.76KK167 pKa = 9.28QKK169 pKa = 10.4KK170 pKa = 9.27SADD173 pKa = 3.3RR174 pKa = 11.84VKK176 pKa = 10.84FINPQNLKK184 pKa = 10.78ALLDD188 pKa = 3.79YY189 pKa = 10.9MEE191 pKa = 4.91TLTPTKK197 pKa = 9.79YY198 pKa = 9.91QYY200 pKa = 11.51YY201 pKa = 8.57YY202 pKa = 11.45DD203 pKa = 3.7NVLYY207 pKa = 10.68CFLLATGCRR216 pKa = 11.84FGEE219 pKa = 4.29AVALEE224 pKa = 4.2WSDD227 pKa = 3.19IDD229 pKa = 5.0LEE231 pKa = 5.16AGTVSISKK239 pKa = 9.17TYY241 pKa = 10.53NRR243 pKa = 11.84QINQISTPKK252 pKa = 8.61TKK254 pKa = 10.09SGKK257 pKa = 10.17RR258 pKa = 11.84IISIDD263 pKa = 3.49NKK265 pKa = 10.74LVLLLKK271 pKa = 10.05QYY273 pKa = 11.05RR274 pKa = 11.84NRR276 pKa = 11.84QRR278 pKa = 11.84LIFLEE283 pKa = 4.2IGARR287 pKa = 11.84APKK290 pKa = 10.42VIFASPTLTYY300 pKa = 10.59ASSDD304 pKa = 3.6VRR306 pKa = 11.84SKK308 pKa = 11.51ALAHH312 pKa = 6.23RR313 pKa = 11.84CKK315 pKa = 10.45EE316 pKa = 3.77VGIPRR321 pKa = 11.84FTFHH325 pKa = 7.74AFRR328 pKa = 11.84HH329 pKa = 4.69THH331 pKa = 6.92ASLLLNAGISYY342 pKa = 10.65KK343 pKa = 10.3EE344 pKa = 3.81LQHH347 pKa = 7.18RR348 pKa = 11.84LGHH351 pKa = 5.95SNIKK355 pKa = 7.93MTLDD359 pKa = 3.46TYY361 pKa = 11.1GHH363 pKa = 6.42ISKK366 pKa = 10.22EE367 pKa = 4.12KK368 pKa = 8.59EE369 pKa = 3.91KK370 pKa = 10.84EE371 pKa = 3.89AVSYY375 pKa = 10.0FEE377 pKa = 5.0KK378 pKa = 10.72AINNLL383 pKa = 3.44

Molecular weight: 44.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

3773

46

464

150.9

17.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.645 ± 0.395

0.822 ± 0.222

5.46 ± 0.457

7.66 ± 0.608

4.055 ± 0.296

4.506 ± 0.366

1.643 ± 0.283

7.13 ± 0.422

9.701 ± 0.353

9.674 ± 0.56

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.855 ± 0.229

5.804 ± 0.375

3.207 ± 0.433

4.612 ± 0.244

5.009 ± 0.397

5.592 ± 0.32

6.387 ± 0.45

5.354 ± 0.337

0.928 ± 0.157

4.956 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski