Erwinia amylovora phage Era103

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Eracentumvirus; Erwinia virus Era103

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A2I800|A2I800_9CAUD Uncharacterized protein OS=Erwinia amylovora phage Era103 OX=418443 GN=Era103g32 PE=4 SV=1
MM1 pKa = 7.25NKK3 pKa = 8.09ITEE6 pKa = 4.32GCTVKK11 pKa = 10.25IVSHH15 pKa = 6.67GYY17 pKa = 9.68VDD19 pKa = 4.53GDD21 pKa = 3.34IDD23 pKa = 3.9THH25 pKa = 6.86LSNYY29 pKa = 8.93PGYY32 pKa = 10.61EE33 pKa = 3.59IGTEE37 pKa = 4.02HH38 pKa = 6.45QVTEE42 pKa = 4.4ISPCGTAIMLGHH54 pKa = 7.59DD55 pKa = 3.14ISVSPEE61 pKa = 3.09EE62 pKa = 3.92VAYY65 pKa = 11.13VNSPDD70 pKa = 3.16

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A2I7Z4|A2I7Z4_9CAUD Uncharacterized protein OS=Erwinia amylovora phage Era103 OX=418443 GN=Era103g26 PE=4 SV=1
MM1 pKa = 7.59SGLSCTGGVIGTEE14 pKa = 3.82GRR16 pKa = 11.84VRR18 pKa = 11.84KK19 pKa = 9.76VLSTWGRR26 pKa = 11.84SGEE29 pKa = 4.38SEE31 pKa = 3.8LTMIKK36 pKa = 9.25TLEE39 pKa = 3.93NLAAFWRR46 pKa = 11.84LNLSPQTEE54 pKa = 4.9YY55 pKa = 10.47IHH57 pKa = 6.25HH58 pKa = 6.08THH60 pKa = 6.09LTGPQRR66 pKa = 11.84MALANKK72 pKa = 8.19EE73 pKa = 3.97ATRR76 pKa = 11.84TSRR79 pKa = 11.84VLRR82 pKa = 11.84RR83 pKa = 11.84KK84 pKa = 9.43NN85 pKa = 3.05

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

13817

37

1294

260.7

28.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.438 ± 0.475

0.753 ± 0.15

6.231 ± 0.247

6.289 ± 0.38

3.358 ± 0.172

7.925 ± 0.321

2.041 ± 0.192

5.008 ± 0.255

5.877 ± 0.263

8.005 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.04 ± 0.148

4.719 ± 0.248

3.915 ± 0.185

4.292 ± 0.296

5.435 ± 0.31

6.051 ± 0.319

5.819 ± 0.383

6.97 ± 0.343

1.469 ± 0.129

3.365 ± 0.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski