Campylobacter virus CP21
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 257 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I7II63|I7II63_9CAUD Putative baseplate hub subunit and tail lysozyme (T4-gp05-like) OS=Campylobacter virus CP21 OX=1190451 GN=CP21_040 PE=4 SV=1
MM1 pKa = 6.82 YY2 pKa = 9.99 VARR5 pKa = 11.84 FNEE8 pKa = 4.17 YY9 pKa = 10.17 FGYY12 pKa = 10.27 DD13 pKa = 3.69 YY14 pKa = 11.5 QLMDD18 pKa = 3.94 VEE20 pKa = 4.74 TVDD23 pKa = 3.5 ILEE26 pKa = 4.93 NIYY29 pKa = 11.06 DD30 pKa = 3.83 SLGYY34 pKa = 9.2 MPRR37 pKa = 11.84 DD38 pKa = 3.63 PEE40 pKa = 5.28 PIDD43 pKa = 3.86 LEE45 pKa = 4.12 IVLEE49 pKa = 4.09 EE50 pKa = 4.85 FYY52 pKa = 10.45 NTDD55 pKa = 3.36 KK56 pKa = 11.01 IYY58 pKa = 10.82 VLCEE62 pKa = 3.82 ADD64 pKa = 3.57 DD65 pKa = 4.27 EE66 pKa = 4.81 EE67 pKa = 6.57 IEE69 pKa = 4.29 FCSCIDD75 pKa = 3.44 EE76 pKa = 4.55 VEE78 pKa = 4.22 PEE80 pKa = 4.22 VIIDD84 pKa = 3.58 FLKK87 pKa = 11.01 SKK89 pKa = 10.1 GHH91 pKa = 5.8 TFMDD95 pKa = 4.27 SNAA98 pKa = 3.62
Molecular weight: 11.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.871
IPC2_protein 3.656
IPC_protein 3.605
Toseland 3.414
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.516
Rodwell 3.439
Grimsley 3.325
Solomon 3.579
Lehninger 3.528
Nozaki 3.719
DTASelect 3.884
Thurlkill 3.465
EMBOSS 3.528
Sillero 3.732
Patrickios 0.146
IPC_peptide 3.567
IPC2_peptide 3.706
IPC2.peptide.svr19 3.698
Protein with the highest isoelectric point:
>tr|I7KLL3|I7KLL3_9CAUD Putative transketolase OS=Campylobacter virus CP21 OX=1190451 GN=CP21_041 PE=4 SV=1
MM1 pKa = 7.71 LEE3 pKa = 4.12 LSNFLRR9 pKa = 11.84 SPFLDD14 pKa = 3.36 MPSISFNNKK23 pKa = 5.66 NTSRR27 pKa = 11.84 FIKK30 pKa = 10.21 KK31 pKa = 9.9 CFRR34 pKa = 11.84 NNGNISIISYY44 pKa = 9.49 FVFNNN49 pKa = 3.15
Molecular weight: 5.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.19
IPC2_protein 9.355
IPC_protein 9.516
Toseland 10.248
ProMoST 9.823
Dawson 10.379
Bjellqvist 10.028
Wikipedia 10.511
Rodwell 10.877
Grimsley 10.423
Solomon 10.452
Lehninger 10.438
Nozaki 10.262
DTASelect 9.999
Thurlkill 10.248
EMBOSS 10.628
Sillero 10.306
Patrickios 10.804
IPC_peptide 10.452
IPC2_peptide 8.887
IPC2.peptide.svr19 8.292
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
257
0
257
51106
34
1283
198.9
22.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.303 ± 0.15
1.716 ± 0.242
6.786 ± 0.117
6.514 ± 0.158
5.222 ± 0.135
4.434 ± 0.126
1.299 ± 0.063
8.735 ± 0.156
9.678 ± 0.18
8.729 ± 0.163
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.871 ± 0.066
8.118 ± 0.148
2.779 ± 0.082
2.865 ± 0.108
3.088 ± 0.098
7.05 ± 0.163
5.647 ± 0.154
5.156 ± 0.143
0.677 ± 0.038
5.334 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here