Salipaludibacillus aurantiacus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Salipaludibacillus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4227 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9TBN7|A0A1H9TBN7_9BACI Uncharacterized protein OS=Salipaludibacillus aurantiacus OX=1601833 GN=SAMN05518684_105206 PE=4 SV=1
MM1 pKa = 7.21KK2 pKa = 10.28KK3 pKa = 10.23KK4 pKa = 8.2YY5 pKa = 9.16TSMFALLLTGSLILAACGEE24 pKa = 4.33GDD26 pKa = 5.07DD27 pKa = 5.6NGNDD31 pKa = 3.56GNNAGGNNNAEE42 pKa = 4.06EE43 pKa = 4.52NEE45 pKa = 4.04ANDD48 pKa = 4.65NGGGDD53 pKa = 4.28DD54 pKa = 4.27NAAADD59 pKa = 3.95TDD61 pKa = 3.98VGNLQLGTGSTGGTYY76 pKa = 10.26YY77 pKa = 10.54PLGQEE82 pKa = 3.91MATVINNNIEE92 pKa = 3.77VDD94 pKa = 3.83GFDD97 pKa = 4.05LSAVSSGASVDD108 pKa = 3.82NIISIFQGEE117 pKa = 4.5MDD119 pKa = 4.37LGMSVHH125 pKa = 6.72LPALDD130 pKa = 3.74SLTGDD135 pKa = 3.53GDD137 pKa = 4.1FEE139 pKa = 4.43GVEE142 pKa = 3.88VDD144 pKa = 3.19NFGFMGHH151 pKa = 7.23IYY153 pKa = 9.81PEE155 pKa = 4.03VMQIVTGADD164 pKa = 3.09SGIEE168 pKa = 4.16SIGDD172 pKa = 3.66LKK174 pKa = 11.21DD175 pKa = 3.29KK176 pKa = 10.78SVAIGPPGSGTQAAAKK192 pKa = 9.84LVLEE196 pKa = 4.77AYY198 pKa = 10.07GLEE201 pKa = 4.52DD202 pKa = 3.69GDD204 pKa = 3.65YY205 pKa = 11.03DD206 pKa = 4.22AYY208 pKa = 10.73EE209 pKa = 4.29EE210 pKa = 4.81GFGDD214 pKa = 3.58AAGRR218 pKa = 11.84IQDD221 pKa = 3.99GNLDD225 pKa = 3.52ASFGLLGLPAGSIEE239 pKa = 4.13EE240 pKa = 4.16LSISRR245 pKa = 11.84DD246 pKa = 2.97IRR248 pKa = 11.84LLPISDD254 pKa = 4.12EE255 pKa = 4.05GLDD258 pKa = 3.81YY259 pKa = 11.05IEE261 pKa = 5.04EE262 pKa = 4.17NSGYY266 pKa = 10.08EE267 pKa = 4.11ALNIPADD274 pKa = 3.76SYY276 pKa = 12.01DD277 pKa = 4.52FMDD280 pKa = 4.76EE281 pKa = 3.92DD282 pKa = 4.04VQAITAYY289 pKa = 10.16AILVGSTDD297 pKa = 3.67TIDD300 pKa = 3.63EE301 pKa = 4.16EE302 pKa = 4.47LGYY305 pKa = 10.46EE306 pKa = 3.68ITKK309 pKa = 10.82ALFEE313 pKa = 4.37HH314 pKa = 6.63SGDD317 pKa = 3.31ISHH320 pKa = 7.27PQGDD324 pKa = 4.27HH325 pKa = 5.11LTKK328 pKa = 10.74EE329 pKa = 3.95NALNGSNGLPIHH341 pKa = 6.9PGAEE345 pKa = 3.52RR346 pKa = 11.84YY347 pKa = 9.37FEE349 pKa = 4.27EE350 pKa = 4.92EE351 pKa = 4.99GITEE355 pKa = 4.02

Molecular weight: 37.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9VN47|A0A1H9VN47_9BACI Putative sporulation protein YyaC OS=Salipaludibacillus aurantiacus OX=1601833 GN=SAMN05518684_111103 PE=4 SV=1
MM1 pKa = 7.69GKK3 pKa = 7.98PTFNPNNRR11 pKa = 11.84KK12 pKa = 9.34RR13 pKa = 11.84KK14 pKa = 8.34KK15 pKa = 9.1NHH17 pKa = 4.73GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37NGRR29 pKa = 11.84KK30 pKa = 9.17VLANRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.69GRR40 pKa = 11.84KK41 pKa = 8.69VLSAA45 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4227

0

4227

1241661

29

2742

293.7

32.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.282 ± 0.052

0.669 ± 0.011

5.528 ± 0.06

8.189 ± 0.053

4.518 ± 0.03

7.197 ± 0.043

2.173 ± 0.019

6.984 ± 0.036

6.388 ± 0.045

9.56 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.855 ± 0.019

4.225 ± 0.027

3.803 ± 0.021

3.497 ± 0.025

4.284 ± 0.029

5.984 ± 0.029

5.358 ± 0.024

7.016 ± 0.032

1.062 ± 0.016

3.427 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski