Wuhan pillworm virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KPP4|A0A1L3KPP4_9VIRU Uncharacterized protein OS=Wuhan pillworm virus 1 OX=1923744 PE=4 SV=1
MM1 pKa = 7.25YY2 pKa = 9.67SSSSASVNDD11 pKa = 3.6SVYY14 pKa = 11.02SEE16 pKa = 5.54DD17 pKa = 5.56DD18 pKa = 3.46LGPVCKK24 pKa = 9.98EE25 pKa = 3.86SMSIPDD31 pKa = 3.77EE32 pKa = 3.96QFLKK36 pKa = 11.06YY37 pKa = 10.45KK38 pKa = 10.33LANFQNFNSHH48 pKa = 5.12NNKK51 pKa = 8.98VLKK54 pKa = 10.48LIEE57 pKa = 4.46GMSKK61 pKa = 10.18DD62 pKa = 3.25SKK64 pKa = 10.79YY65 pKa = 10.88FRR67 pKa = 11.84DD68 pKa = 4.17DD69 pKa = 3.3VLNSQLRR76 pKa = 11.84FHH78 pKa = 7.41PNLEE82 pKa = 4.74SISEE86 pKa = 4.35LYY88 pKa = 8.48FTLSDD93 pKa = 3.26IKK95 pKa = 11.39VKK97 pKa = 10.63ISYY100 pKa = 10.68DD101 pKa = 3.15STANLLEE108 pKa = 4.34ITSDD112 pKa = 3.17ACKK115 pKa = 10.43GGGMKK120 pKa = 10.32SIHH123 pKa = 6.84LNDD126 pKa = 3.95TEE128 pKa = 5.67DD129 pKa = 3.78KK130 pKa = 11.1VDD132 pKa = 3.4YY133 pKa = 10.53NLNIGKK139 pKa = 9.77GLINYY144 pKa = 7.73LWPLFDD150 pKa = 4.26RR151 pKa = 11.84VEE153 pKa = 4.18VCQINCINTFKK164 pKa = 10.89SKK166 pKa = 10.34KK167 pKa = 9.68DD168 pKa = 3.47CMCIEE173 pKa = 4.28SKK175 pKa = 11.15YY176 pKa = 10.8LVLDD180 pKa = 3.72RR181 pKa = 11.84CDD183 pKa = 3.95EE184 pKa = 3.99MDD186 pKa = 3.55EE187 pKa = 4.48LRR189 pKa = 11.84FKK191 pKa = 10.08ATQANGDD198 pKa = 3.87VNDD201 pKa = 4.48MDD203 pKa = 5.47FLLQKK208 pKa = 9.81EE209 pKa = 4.16RR210 pKa = 11.84SIVTMLALVMLAKK223 pKa = 10.16EE224 pKa = 4.46KK225 pKa = 10.85PEE227 pKa = 4.07DD228 pKa = 3.63MCEE231 pKa = 3.82EE232 pKa = 4.14TASKK236 pKa = 10.27IARR239 pKa = 11.84LLTKK243 pKa = 10.09KK244 pKa = 10.15HH245 pKa = 5.91NNVLNGIPDD254 pKa = 3.53VLQFCMIEE262 pKa = 3.85HH263 pKa = 7.45LYY265 pKa = 10.84NEE267 pKa = 5.34SILSDD272 pKa = 3.62YY273 pKa = 10.53KK274 pKa = 10.72SEE276 pKa = 4.19YY277 pKa = 8.68VLEE280 pKa = 4.17YY281 pKa = 10.23MMNPASYY288 pKa = 10.7LSAKK292 pKa = 9.94ISAISDD298 pKa = 3.23ICDD301 pKa = 2.9KK302 pKa = 11.13KK303 pKa = 11.14GFNIGYY309 pKa = 8.67SSYY312 pKa = 10.52RR313 pKa = 11.84PGYY316 pKa = 10.56ASLITHH322 pKa = 7.18ANYY325 pKa = 9.99INEE328 pKa = 4.3CKK330 pKa = 9.96IGHH333 pKa = 6.26LTSLSDD339 pKa = 4.9KK340 pKa = 10.71ILTEE344 pKa = 4.68KK345 pKa = 10.81GFALCKK351 pKa = 9.98TDD353 pKa = 3.21GTLLKK358 pKa = 10.57KK359 pKa = 9.75RR360 pKa = 11.84TLKK363 pKa = 10.55PRR365 pKa = 11.84TFALDD370 pKa = 3.07IALDD374 pKa = 3.45WRR376 pKa = 11.84NLYY379 pKa = 10.35DD380 pKa = 5.22KK381 pKa = 11.18SIFYY385 pKa = 10.93CFDD388 pKa = 5.04LISFKK393 pKa = 10.67IGGLSDD399 pKa = 3.31QALINPVNFRR409 pKa = 11.84CINSEE414 pKa = 4.08KK415 pKa = 10.66VSLLRR420 pKa = 11.84DD421 pKa = 3.54LNYY424 pKa = 10.12ISLTSADD431 pKa = 4.11VIEE434 pKa = 5.13DD435 pKa = 3.39EE436 pKa = 4.81CKK438 pKa = 10.71VRR440 pKa = 11.84NQPKK444 pKa = 10.09SVVVKK449 pKa = 10.52IKK451 pKa = 10.93NNSSLVKK458 pKa = 10.11VHH460 pKa = 6.18SEE462 pKa = 3.89CLEE465 pKa = 3.97RR466 pKa = 11.84AHH468 pKa = 7.08IRR470 pKa = 3.5

Molecular weight: 53.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KPQ5|A0A1L3KPQ5_9VIRU Putative glycoprotein OS=Wuhan pillworm virus 1 OX=1923744 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.32VCQFKK7 pKa = 9.28LTPRR11 pKa = 11.84NGKK14 pKa = 9.36FISGIFPGKK23 pKa = 9.72IIKK26 pKa = 9.93SLYY29 pKa = 9.4DD30 pKa = 3.16VSVATGYY37 pKa = 11.13LEE39 pKa = 4.21LAAYY43 pKa = 8.89PDD45 pKa = 5.05GYY47 pKa = 10.46QDD49 pKa = 3.7RR50 pKa = 11.84SDD52 pKa = 3.81YY53 pKa = 11.07MIDD56 pKa = 3.33TVVKK60 pKa = 9.93VIQGLGKK67 pKa = 9.41KK68 pKa = 9.94ALAAVHH74 pKa = 6.97KK75 pKa = 9.11IQSSSEE81 pKa = 4.08LNSSEE86 pKa = 4.21LDD88 pKa = 3.37EE89 pKa = 4.34MNITIQEE96 pKa = 4.15RR97 pKa = 11.84LHH99 pKa = 7.4DD100 pKa = 4.52EE101 pKa = 4.9LNDD104 pKa = 3.49DD105 pKa = 5.04HH106 pKa = 7.13LWPMPTGNVEE116 pKa = 4.03STLNVPDD123 pKa = 4.26DD124 pKa = 4.17TIKK127 pKa = 10.14TLNEE131 pKa = 2.94IAAFMKK137 pKa = 10.42EE138 pKa = 4.17MSPSANTNNKK148 pKa = 9.34VSDD151 pKa = 3.8RR152 pKa = 11.84DD153 pKa = 3.51ALEE156 pKa = 3.88QLEE159 pKa = 4.63SDD161 pKa = 4.08LMAYY165 pKa = 8.51EE166 pKa = 3.77QFGIIGCSNLIKK178 pKa = 10.0RR179 pKa = 11.84TVAGKK184 pKa = 9.3QAAGTLVTGILRR196 pKa = 11.84MKK198 pKa = 10.65NSGMDD203 pKa = 3.52DD204 pKa = 3.43NSIKK208 pKa = 10.44RR209 pKa = 11.84WIKK212 pKa = 10.43NKK214 pKa = 9.73SDD216 pKa = 3.14KK217 pKa = 11.6AMDD220 pKa = 3.86EE221 pKa = 4.48LGDD224 pKa = 3.62EE225 pKa = 4.62AFASAEE231 pKa = 4.02VLSRR235 pKa = 11.84EE236 pKa = 4.22GAAGQLAAQEE246 pKa = 4.45KK247 pKa = 9.52NISVMAWLTIVGILGGYY264 pKa = 9.84DD265 pKa = 3.58SEE267 pKa = 4.88GKK269 pKa = 10.53ALTTTNKK276 pKa = 9.84LSKK279 pKa = 10.11DD280 pKa = 2.95IKK282 pKa = 10.15EE283 pKa = 4.39FCDD286 pKa = 3.64VMRR289 pKa = 11.84KK290 pKa = 9.59CLMIDD295 pKa = 3.21RR296 pKa = 11.84SKK298 pKa = 11.04RR299 pKa = 11.84RR300 pKa = 11.84VIIEE304 pKa = 3.81ASGSSFTKK312 pKa = 10.43KK313 pKa = 8.08VWTMARR319 pKa = 11.84TSLRR323 pKa = 11.84LNCCTLILSEE333 pKa = 3.97TRR335 pKa = 11.84MVQTCVVTGLGTALSNIRR353 pKa = 11.84NVKK356 pKa = 10.13ADD358 pKa = 3.26TGFVRR363 pKa = 11.84FLCEE367 pKa = 3.1MWLAIEE373 pKa = 4.8SVPEE377 pKa = 3.9DD378 pKa = 3.65PRR380 pKa = 11.84LKK382 pKa = 10.63KK383 pKa = 10.37DD384 pKa = 3.48DD385 pKa = 4.41FKK387 pKa = 11.26MQVASLLQHH396 pKa = 6.14SLVSAGNCFHH406 pKa = 7.32FSIKK410 pKa = 9.69MSGRR414 pKa = 11.84ASDD417 pKa = 3.91VRR419 pKa = 11.84IKK421 pKa = 10.66QGNN424 pKa = 3.16

Molecular weight: 46.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

5541

424

3387

1385.3

157.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.385 ± 0.582

2.581 ± 0.608

6.641 ± 0.317

6.136 ± 0.221

3.447 ± 0.444

4.656 ± 0.653

1.66 ± 0.082

7.472 ± 0.117

7.219 ± 0.468

10.341 ± 0.384

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.267 ± 0.154

5.829 ± 0.254

2.527 ± 0.252

2.581 ± 0.131

5.45 ± 0.49

10.161 ± 0.586

5.198 ± 0.419

5.938 ± 0.565

1.191 ± 0.131

3.321 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski