Tigriopus californicus (Marine copepod)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Crustacea; Multicrustacea; Hexanauplia; Copepoda; Neocopepoda; Podoplea; Harpacticoida; Harpacticidae; Tigriopus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15551 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A553PF11|A0A553PF11_TIGCA Uncharacterized protein OS=Tigriopus californicus OX=6832 GN=TCAL_14718 PE=4 SV=1
MM1 pKa = 7.48NNRR4 pKa = 11.84EE5 pKa = 4.22FILVKK10 pKa = 10.38ISLVLVLLIPPGEE23 pKa = 4.1LSPRR27 pKa = 11.84PLSVDD32 pKa = 3.68DD33 pKa = 3.85EE34 pKa = 4.63FSRR37 pKa = 11.84VDD39 pKa = 3.87FGPIARR45 pKa = 11.84NDD47 pKa = 3.52YY48 pKa = 10.86DD49 pKa = 3.84QGDD52 pKa = 3.98TGDD55 pKa = 3.71EE56 pKa = 4.2TLGGSNTAFGFADD69 pKa = 3.44HH70 pKa = 6.72QEE72 pKa = 4.08DD73 pKa = 4.41SFTSFGAPAASVSDD87 pKa = 4.01YY88 pKa = 11.59YY89 pKa = 11.61GDD91 pKa = 5.42DD92 pKa = 3.2EE93 pKa = 5.01DD94 pKa = 5.66KK95 pKa = 11.03RR96 pKa = 11.84VAGPAYY102 pKa = 10.38GGTAPNNYY110 pKa = 9.92DD111 pKa = 3.59YY112 pKa = 11.45GSEE115 pKa = 4.13GTDD118 pKa = 4.01GIGPQDD124 pKa = 3.47GGQYY128 pKa = 10.25QDD130 pKa = 4.25NLPHH134 pKa = 6.95PGVDD138 pKa = 3.17GQAYY142 pKa = 9.2PNYY145 pKa = 9.21EE146 pKa = 3.91DD147 pKa = 5.3DD148 pKa = 4.2YY149 pKa = 10.91LRR151 pKa = 11.84VGAPSPSDD159 pKa = 3.24PFDD162 pKa = 3.46FF163 pKa = 5.36

Molecular weight: 17.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A553PSW2|A0A553PSW2_TIGCA Glycosyltransferase family 92 protein (Fragment) OS=Tigriopus californicus OX=6832 GN=TCAL_12012 PE=3 SV=1
MM1 pKa = 7.58LNLGISKK8 pKa = 10.05SPRR11 pKa = 11.84GIMSNAPLRR20 pKa = 11.84PRR22 pKa = 11.84SNIHH26 pKa = 6.09PNKK29 pKa = 9.46LTLKK33 pKa = 10.56LLIWLLWIVAWHH45 pKa = 5.59HH46 pKa = 4.96VNRR49 pKa = 11.84RR50 pKa = 11.84MSFFLNQTSLAPP62 pKa = 3.93

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15551

0

15551

8076027

22

9221

519.3

58.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.258 ± 0.016

1.965 ± 0.019

5.644 ± 0.013

6.447 ± 0.024

4.114 ± 0.016

6.232 ± 0.022

2.723 ± 0.01

5.132 ± 0.016

5.78 ± 0.022

9.068 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.009

4.605 ± 0.013

5.704 ± 0.027

4.455 ± 0.015

5.355 ± 0.016

8.699 ± 0.026

5.518 ± 0.014

5.988 ± 0.014

1.172 ± 0.007

2.701 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski