Desulfurispirillum indicum (strain ATCC BAA-1389 / DSM 22839 / S5)

Taxonomy: cellular organisms; Bacteria; Chrysiogenetes; Chrysiogenetes; Chrysiogenales; Chrysiogenaceae; Desulfurispirillum; Desulfurispirillum indicum

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2551 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6W658|E6W658_DESIS Flagellar motor switch protein FliN OS=Desulfurispirillum indicum (strain ATCC BAA-1389 / DSM 22839 / S5) OX=653733 GN=Selin_1359 PE=3 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84AVTLIFSMLFLLVGCSEE19 pKa = 4.85MLPEE23 pKa = 4.82SPGAGKK29 pKa = 10.53ASVNISIKK37 pKa = 10.61RR38 pKa = 11.84PVNQIQEE45 pKa = 4.39DD46 pKa = 4.0RR47 pKa = 11.84LQAAFIDD54 pKa = 4.23DD55 pKa = 3.55RR56 pKa = 11.84TMQLDD61 pKa = 3.35IFIYY65 pKa = 8.83PFPFGSYY72 pKa = 10.89DD73 pKa = 5.25DD74 pKa = 3.54IVKK77 pKa = 9.99LQRR80 pKa = 11.84CIVAAWSMSSEE91 pKa = 4.09GEE93 pKa = 3.85PTYY96 pKa = 11.37GEE98 pKa = 4.53IITPEE103 pKa = 3.61IRR105 pKa = 11.84STCNVPGDD113 pKa = 3.97FDD115 pKa = 4.31SAGQMAMSMSAVRR128 pKa = 11.84EE129 pKa = 3.62VSIPYY134 pKa = 10.43GSGTVNTQISEE145 pKa = 4.46LEE147 pKa = 4.15PGSYY151 pKa = 9.33MIAVIQSGAYY161 pKa = 8.74GEE163 pKa = 4.48EE164 pKa = 4.08MSKK167 pKa = 10.39VRR169 pKa = 11.84TYY171 pKa = 10.42ATLEE175 pKa = 4.17PGPNNIVINLLHH187 pKa = 6.28GKK189 pKa = 8.22WVFVNEE195 pKa = 4.35SDD197 pKa = 4.99DD198 pKa = 3.99PAPLEE203 pKa = 4.05LQLLHH208 pKa = 5.92KK209 pKa = 9.7TEE211 pKa = 4.61FFVEE215 pKa = 4.35DD216 pKa = 4.07SEE218 pKa = 4.64PVGLVEE224 pKa = 5.65SPAAALGFSGSGTVQLEE241 pKa = 3.47AVYY244 pKa = 10.38LHH246 pKa = 6.42EE247 pKa = 4.47TLDD250 pKa = 3.94IPEE253 pKa = 5.22HH254 pKa = 6.6IYY256 pKa = 10.97LDD258 pKa = 3.97AYY260 pKa = 10.75LGYY263 pKa = 10.39HH264 pKa = 6.73GSNLFGQYY272 pKa = 11.05DD273 pKa = 3.85NASARR278 pKa = 11.84YY279 pKa = 8.17YY280 pKa = 11.56GLMDD284 pKa = 3.47NHH286 pKa = 6.87AMSFIAAEE294 pKa = 4.07QVDD297 pKa = 4.47AVASTEE303 pKa = 3.94PAFIMQQYY311 pKa = 8.65FAGASSHH318 pKa = 6.8KK319 pKa = 9.09ITLGEE324 pKa = 3.85LMGWSGIDD332 pKa = 3.23NAFGLIFYY340 pKa = 9.97GADD343 pKa = 2.95GTGFLNSLAKK353 pKa = 7.79TTKK356 pKa = 10.21NLIYY360 pKa = 10.09NTDD363 pKa = 4.09FEE365 pKa = 4.89QIDD368 pKa = 3.73IQHH371 pKa = 4.77YY372 pKa = 8.63WYY374 pKa = 10.63RR375 pKa = 11.84EE376 pKa = 3.94DD377 pKa = 3.94LSEE380 pKa = 4.47PGFPVIEE387 pKa = 4.37LDD389 pKa = 3.9VMVSEE394 pKa = 5.08HH395 pKa = 6.72SISPFADD402 pKa = 3.08TSEE405 pKa = 4.39VVQKK409 pKa = 11.19LMGLDD414 pKa = 3.01SGEE417 pKa = 4.78RR418 pKa = 11.84ITGTLVEE425 pKa = 4.36YY426 pKa = 10.57FSTEE430 pKa = 3.93RR431 pKa = 11.84EE432 pKa = 4.29GGTVGPVSPPVIDD445 pKa = 4.52YY446 pKa = 10.42DD447 pKa = 3.89PKK449 pKa = 11.27DD450 pKa = 3.82GFSVASASSSVVPALLNRR468 pKa = 11.84ATLTRR473 pKa = 11.84MLQQQLALEE482 pKa = 4.16QAEE485 pKa = 4.27KK486 pKa = 11.08DD487 pKa = 3.69LGIGTITAASNQPGPKK503 pKa = 9.75CFTSDD508 pKa = 2.81TSRR511 pKa = 11.84AYY513 pKa = 8.53TWLLYY518 pKa = 10.14RR519 pKa = 11.84YY520 pKa = 9.44NGTSWVPGYY529 pKa = 10.18EE530 pKa = 3.95EE531 pKa = 4.07SGEE534 pKa = 4.23FIASNDD540 pKa = 3.48NLDD543 pKa = 4.02FEE545 pKa = 5.74AEE547 pKa = 4.23EE548 pKa = 4.49FPVIQAEE555 pKa = 4.43ICLHH559 pKa = 6.81PIRR562 pKa = 11.84LAAQQPAPEE571 pKa = 4.75EE572 pKa = 4.63GPSNLIAHH580 pKa = 5.66YY581 pKa = 9.57TFDD584 pKa = 4.55GNVADD589 pKa = 4.98VKK591 pKa = 10.71GAYY594 pKa = 9.89DD595 pKa = 3.68GASSVAGVTFDD606 pKa = 4.22AGRR609 pKa = 11.84FGQAVYY615 pKa = 11.03LDD617 pKa = 3.97GGYY620 pKa = 8.06ITLPHH625 pKa = 7.41EE626 pKa = 4.54YY627 pKa = 10.43NPIISPDD634 pKa = 3.36AEE636 pKa = 4.1EE637 pKa = 4.7FSISTWIKK645 pKa = 9.92VDD647 pKa = 3.91EE648 pKa = 4.8LPEE651 pKa = 6.23ADD653 pKa = 4.54DD654 pKa = 3.23EE655 pKa = 4.58WVIFNHH661 pKa = 5.61GWGEE665 pKa = 3.9FKK667 pKa = 10.82LALNHH672 pKa = 6.18TGSVSFAVNEE682 pKa = 4.07DD683 pKa = 3.01QGYY686 pKa = 8.51GAWRR690 pKa = 11.84TIEE693 pKa = 4.27GPVPLGEE700 pKa = 4.33FVHH703 pKa = 6.69IAAIYY708 pKa = 10.31SRR710 pKa = 11.84DD711 pKa = 3.17NGMQLWVNGAGVSFYY726 pKa = 11.4NSLINGMRR734 pKa = 11.84DD735 pKa = 2.94PDD737 pKa = 4.29GIYY740 pKa = 10.4RR741 pKa = 11.84FIGFGRR747 pKa = 11.84SSDD750 pKa = 3.31EE751 pKa = 4.96DD752 pKa = 4.12GPFFQGAIDD761 pKa = 3.53EE762 pKa = 4.4TRR764 pKa = 11.84IFDD767 pKa = 3.86RR768 pKa = 11.84ALTEE772 pKa = 4.15AEE774 pKa = 4.29VFLLADD780 pKa = 5.75AITGTAGNDD789 pKa = 3.46NLIGTSNNDD798 pKa = 2.64IFIGSAGDD806 pKa = 4.18DD807 pKa = 3.76IIDD810 pKa = 3.86GVGGVNTVVYY820 pKa = 10.22VGNHH824 pKa = 4.68TLYY827 pKa = 10.65QVIDD831 pKa = 3.89NGGGSYY837 pKa = 9.25TVVKK841 pKa = 10.35PDD843 pKa = 3.54GTDD846 pKa = 2.74TLTNIQYY853 pKa = 11.02LSFGDD858 pKa = 3.7VQNIAITDD866 pKa = 4.14LLSEE870 pKa = 4.61NIVVIDD876 pKa = 4.97CGMDD880 pKa = 3.35DD881 pKa = 5.19CSWIPAGRR889 pKa = 11.84FAFPDD894 pKa = 3.76DD895 pKa = 4.88GEE897 pKa = 4.4FSLNDD902 pKa = 3.64PYY904 pKa = 11.62VRR906 pKa = 11.84LVPAQLQCEE915 pKa = 4.48PPNPLCWDD923 pKa = 4.19NIVCSVDD930 pKa = 2.95GSTGEE935 pKa = 4.15FGSGNSTCYY944 pKa = 10.2IFSSDD949 pKa = 4.26PGALQNAVNLSEE961 pKa = 4.8DD962 pKa = 3.39FQLILFDD969 pKa = 4.31NDD971 pKa = 3.72DD972 pKa = 3.83RR973 pKa = 11.84DD974 pKa = 4.16VPNHH978 pKa = 6.29DD979 pKa = 5.17LEE981 pKa = 4.7DD982 pKa = 3.52AVYY985 pKa = 10.85LIMSDD990 pKa = 4.05FLPGEE995 pKa = 4.37TIEE998 pKa = 4.28GAVVVEE1004 pKa = 4.3YY1005 pKa = 10.93EE1006 pKa = 4.32EE1007 pKa = 4.06NRR1009 pKa = 11.84HH1010 pKa = 5.96FISAAEE1016 pKa = 3.78AFLFNFFSSEE1026 pKa = 4.14LDD1028 pKa = 3.41VQTYY1032 pKa = 9.41LWIEE1036 pKa = 3.9AAPGFRR1042 pKa = 11.84YY1043 pKa = 8.8TEE1045 pKa = 3.75TWNPSEE1051 pKa = 4.34VFFGHH1056 pKa = 7.0GGTEE1060 pKa = 4.06SLPLFSGTLDD1070 pKa = 3.43TATSLMGDD1078 pKa = 3.83PFEE1081 pKa = 6.41DD1082 pKa = 2.83IHH1084 pKa = 7.92SSHH1087 pKa = 5.65WVYY1090 pKa = 10.48FRR1092 pKa = 11.84SGSLVFGEE1100 pKa = 4.44NPNVYY1105 pKa = 9.83DD1106 pKa = 5.04VNIHH1110 pKa = 5.56WFSNIPMFAPLNDD1123 pKa = 3.89TGISTCVTDD1132 pKa = 4.91DD1133 pKa = 4.02DD1134 pKa = 6.2LIFGTSDD1141 pKa = 3.28CTVGPEE1147 pKa = 4.75IPLQDD1152 pKa = 4.24AYY1154 pKa = 11.12HH1155 pKa = 6.07GTNSFAFEE1163 pKa = 4.83KK1164 pKa = 9.95IDD1166 pKa = 3.42ASGAVLSEE1174 pKa = 4.68AGPDD1178 pKa = 3.3DD1179 pKa = 4.04WDD1181 pKa = 3.72CVRR1184 pKa = 11.84DD1185 pKa = 3.88NTTGLLWQADD1195 pKa = 4.23GNQSGIWDD1203 pKa = 4.4NFPLPDD1209 pKa = 4.57SGFCGCEE1216 pKa = 3.56KK1217 pKa = 9.89WRR1219 pKa = 11.84VPARR1223 pKa = 11.84EE1224 pKa = 4.1EE1225 pKa = 3.82LRR1227 pKa = 11.84SLIDD1231 pKa = 3.46YY1232 pKa = 11.06QMLLDD1237 pKa = 5.07DD1238 pKa = 4.91EE1239 pKa = 5.37APAISDD1245 pKa = 3.56VFFPDD1250 pKa = 4.65SIMSFYY1256 pKa = 10.34WSSTGDD1262 pKa = 3.62DD1263 pKa = 3.14THH1265 pKa = 7.66AWAVSFEE1272 pKa = 4.11TGMEE1276 pKa = 3.96EE1277 pKa = 4.6LKK1279 pKa = 10.8VRR1281 pKa = 11.84NIAEE1285 pKa = 3.87AWGYY1289 pKa = 10.53RR1290 pKa = 11.84AVCAPYY1296 pKa = 10.69

Molecular weight: 141.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6W2F1|E6W2F1_DESIS Histidinol dehydrogenase OS=Desulfurispirillum indicum (strain ATCC BAA-1389 / DSM 22839 / S5) OX=653733 GN=hisD PE=3 SV=1
MM1 pKa = 6.26QQRR4 pKa = 11.84TFRR7 pKa = 11.84PNNRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.73KK14 pKa = 8.77VKK16 pKa = 9.36QGFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37NGRR29 pKa = 11.84KK30 pKa = 8.96VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.37GRR40 pKa = 11.84AVLAAA45 pKa = 4.31

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2551

0

2551

853656

31

1601

334.6

37.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.907 ± 0.049

1.181 ± 0.021

5.148 ± 0.031

6.58 ± 0.041

4.144 ± 0.03

7.253 ± 0.04

2.622 ± 0.026

6.367 ± 0.042

3.88 ± 0.049

10.396 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.76 ± 0.022

3.257 ± 0.036

4.344 ± 0.034

4.406 ± 0.035

6.371 ± 0.043

6.323 ± 0.036

5.172 ± 0.032

6.909 ± 0.036

1.039 ± 0.016

2.939 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski