Bean golden yellow mosaic virus (isolate Puerto Rico) (BGYMV) (Bean golden mosaic virus (isolate Puerto Rico))

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Bean golden yellow mosaic virus

Average proteome isoelectric point is 8.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P0CK41|NSP_BGYMV Nuclear shuttle protein OS=Bean golden yellow mosaic virus (isolate Puerto Rico) OX=222448 GN=BR1 PE=3 SV=1
MM1 pKa = 7.94PPPQRR6 pKa = 11.84FRR8 pKa = 11.84VQSKK12 pKa = 10.67NYY14 pKa = 8.0FLTYY18 pKa = 9.27PRR20 pKa = 11.84CTIPKK25 pKa = 9.85EE26 pKa = 3.98EE27 pKa = 5.24ALSQLQKK34 pKa = 10.6IHH36 pKa = 5.55TTTNKK41 pKa = 10.13KK42 pKa = 9.85FIKK45 pKa = 10.08VCEE48 pKa = 3.97EE49 pKa = 3.6RR50 pKa = 11.84HH51 pKa = 6.79DD52 pKa = 3.77NGEE55 pKa = 4.06PHH57 pKa = 6.77LHH59 pKa = 6.7ALIQFEE65 pKa = 4.82GKK67 pKa = 9.97FICTNKK73 pKa = 10.33RR74 pKa = 11.84LFDD77 pKa = 3.98LVSTTRR83 pKa = 11.84SAHH86 pKa = 4.94FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.04SSSDD99 pKa = 3.07VKK101 pKa = 10.98EE102 pKa = 4.53YY103 pKa = 10.36IDD105 pKa = 3.91KK106 pKa = 11.23DD107 pKa = 3.59GVTIEE112 pKa = 3.92WGQFQVDD119 pKa = 3.41GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQSANDD132 pKa = 3.98SYY134 pKa = 12.06AKK136 pKa = 10.56ALNADD141 pKa = 4.31SIEE144 pKa = 4.23SALTILKK151 pKa = 10.12EE152 pKa = 4.2EE153 pKa = 4.03QPKK156 pKa = 10.91DD157 pKa = 3.6YY158 pKa = 11.08VLQNHH163 pKa = 6.96NIRR166 pKa = 11.84SNLEE170 pKa = 3.8RR171 pKa = 11.84IFFKK175 pKa = 11.01VPEE178 pKa = 4.16PWVPPFPLSSFVNIPVVMQDD198 pKa = 2.6WVDD201 pKa = 3.64DD202 pKa = 3.86YY203 pKa = 11.56FGRR206 pKa = 11.84GSAARR211 pKa = 11.84PEE213 pKa = 4.12RR214 pKa = 11.84PISIIVEE221 pKa = 4.05GDD223 pKa = 3.08SRR225 pKa = 11.84TGKK228 pKa = 8.55TMWARR233 pKa = 11.84ALGPHH238 pKa = 6.63NYY240 pKa = 10.14LSGHH244 pKa = 6.94LDD246 pKa = 3.72FNSLVYY252 pKa = 10.91SNSVEE257 pKa = 4.08YY258 pKa = 10.88NVIDD262 pKa = 6.05DD263 pKa = 3.75ITPNYY268 pKa = 10.34LKK270 pKa = 10.99LKK272 pKa = 9.79DD273 pKa = 3.39WKK275 pKa = 10.79EE276 pKa = 4.0LIGEE280 pKa = 4.19QKK282 pKa = 10.57DD283 pKa = 3.37WQSNCKK289 pKa = 9.38YY290 pKa = 10.21GKK292 pKa = 9.18PVQIKK297 pKa = 10.19GGIPSIVLCNPGEE310 pKa = 4.21GSSYY314 pKa = 11.32KK315 pKa = 10.77DD316 pKa = 3.31FLNKK320 pKa = 9.79EE321 pKa = 4.28EE322 pKa = 5.48KK323 pKa = 9.88PALHH327 pKa = 6.55NWTIHH332 pKa = 4.93NAIFVTLTAPLYY344 pKa = 10.54QSTAQDD350 pKa = 3.73CQTT353 pKa = 3.7

Molecular weight: 40.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0CK43|MVP_BGYMV Movement protein BC1 OS=Bean golden yellow mosaic virus (isolate Puerto Rico) OX=222448 GN=BC1 PE=3 SV=1
MM1 pKa = 7.13YY2 pKa = 10.12ASKK5 pKa = 10.81YY6 pKa = 9.98KK7 pKa = 10.37RR8 pKa = 11.84GSSNYY13 pKa = 6.9QRR15 pKa = 11.84RR16 pKa = 11.84GYY18 pKa = 10.58SRR20 pKa = 11.84YY21 pKa = 9.13QGFRR25 pKa = 11.84RR26 pKa = 11.84TAIVTRR32 pKa = 11.84HH33 pKa = 5.61DD34 pKa = 3.63GKK36 pKa = 10.7RR37 pKa = 11.84RR38 pKa = 11.84QHH40 pKa = 5.91QSNKK44 pKa = 10.01SNEE47 pKa = 4.02DD48 pKa = 2.92PKK50 pKa = 11.01MLVQCIRR57 pKa = 11.84EE58 pKa = 4.17NQFGPDD64 pKa = 3.09FVMSHH69 pKa = 5.33NTAISTFINYY79 pKa = 7.5PQLGKK84 pKa = 10.05IEE86 pKa = 4.35PNRR89 pKa = 11.84CRR91 pKa = 11.84SYY93 pKa = 11.15IKK95 pKa = 10.52LKK97 pKa = 10.32RR98 pKa = 11.84LRR100 pKa = 11.84FKK102 pKa = 10.51GTVKK106 pKa = 10.01IEE108 pKa = 4.01RR109 pKa = 11.84MHH111 pKa = 6.73TDD113 pKa = 2.51VNMDD117 pKa = 3.49GLSPKK122 pKa = 10.01IEE124 pKa = 4.04GVFSIVIVVDD134 pKa = 4.65RR135 pKa = 11.84KK136 pKa = 9.74PHH138 pKa = 6.42LSPSGCLHH146 pKa = 6.31TFDD149 pKa = 4.85EE150 pKa = 5.14LFGARR155 pKa = 11.84INSHH159 pKa = 6.04GNLAVMPSLKK169 pKa = 10.14DD170 pKa = 3.09RR171 pKa = 11.84FYY173 pKa = 10.87IRR175 pKa = 11.84HH176 pKa = 5.63LLKK179 pKa = 10.59RR180 pKa = 11.84VLSVDD185 pKa = 3.52KK186 pKa = 9.54DD187 pKa = 3.38TTMIDD192 pKa = 3.63VEE194 pKa = 4.59GSTLLSNKK202 pKa = 9.27RR203 pKa = 11.84YY204 pKa = 10.63NMWSTFNDD212 pKa = 3.55FDD214 pKa = 4.67HH215 pKa = 7.53DD216 pKa = 4.32SCNGVYY222 pKa = 10.93ANIAKK227 pKa = 10.07NALLVYY233 pKa = 7.78YY234 pKa = 9.96CWMSDD239 pKa = 3.01IMSKK243 pKa = 11.0ASTFVSYY250 pKa = 11.27DD251 pKa = 3.2LDD253 pKa = 4.02YY254 pKa = 11.74VGG256 pKa = 5.53

Molecular weight: 29.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1535

79

353

219.3

25.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.821 ± 0.369

1.824 ± 0.302

5.537 ± 0.412

4.039 ± 0.457

4.365 ± 0.299

5.081 ± 0.485

3.909 ± 0.394

6.71 ± 0.547

6.84 ± 0.251

6.515 ± 0.696

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.215 ± 0.632

6.059 ± 0.786

4.951 ± 0.54

4.625 ± 0.588

6.254 ± 0.641

9.577 ± 1.184

5.212 ± 0.516

5.863 ± 0.722

1.433 ± 0.219

4.169 ± 0.566
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski