Halorubrum pleomorphic virus 3

Taxonomy: Viruses; Monodnaviria; Trapavirae; Saleviricota; Huolimaviricetes; Haloruvirales; Pleolipoviridae; Betapleolipovirus; Betapleolipovirus HRPV3

Average proteome isoelectric point is 5.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9ABN1|H9ABN1_9VIRU VP2 OS=Halorubrum pleomorphic virus 3 OX=1156720 GN=2 PE=4 SV=1
MM1 pKa = 7.3ATSKK5 pKa = 10.99LSGFAGGPGEE15 pKa = 4.9IDD17 pKa = 4.62PIDD20 pKa = 3.78LAALIGIPWFAATIFGVFTFNINVFGGYY48 pKa = 10.17DD49 pKa = 3.86FSAPIWTVAGADD61 pKa = 3.23ISVALLLVLMGTVWVVATNEE81 pKa = 4.09LTKK84 pKa = 10.4TDD86 pKa = 4.02YY87 pKa = 11.55DD88 pKa = 3.84PVYY91 pKa = 10.49LALIGFALLSPLLFVFVPAFEE112 pKa = 5.17SLVTWSDD119 pKa = 3.26LTRR122 pKa = 11.84LAFTIAVSAATTAVSYY138 pKa = 10.19LAA140 pKa = 4.77

Molecular weight: 14.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9ABN6|H9ABN6_9VIRU ORF7 OS=Halorubrum pleomorphic virus 3 OX=1156720 GN=ORF7 PE=4 SV=1
MM1 pKa = 7.67PSHH4 pKa = 7.2RR5 pKa = 11.84ANAHH9 pKa = 4.52GTACEE14 pKa = 3.62YY15 pKa = 10.97HH16 pKa = 6.69LADD19 pKa = 4.19KK20 pKa = 11.2YY21 pKa = 10.92GVRR24 pKa = 11.84LKK26 pKa = 11.04DD27 pKa = 3.43GDD29 pKa = 3.67GTRR32 pKa = 11.84IDD34 pKa = 3.85TSWFDD39 pKa = 3.52GLKK42 pKa = 10.82DD43 pKa = 3.49GTPWEE48 pKa = 4.75FKK50 pKa = 9.54ATARR54 pKa = 11.84RR55 pKa = 11.84HH56 pKa = 6.04ADD58 pKa = 3.3GNPGNFKK65 pKa = 10.12IYY67 pKa = 10.49KK68 pKa = 8.91KK69 pKa = 10.76YY70 pKa = 10.23HH71 pKa = 6.36GKK73 pKa = 9.16LQSNGGRR80 pKa = 11.84YY81 pKa = 8.84GFAVYY86 pKa = 10.03RR87 pKa = 11.84VRR89 pKa = 11.84GTGTQVLGTKK99 pKa = 8.84TVAASRR105 pKa = 11.84LPTVRR110 pKa = 11.84WHH112 pKa = 6.64GGGSHH117 pKa = 7.62RR118 pKa = 11.84DD119 pKa = 3.42TQQAKK124 pKa = 9.94IPISAIFF131 pKa = 3.47

Molecular weight: 14.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

2661

41

613

221.8

24.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.794 ± 0.67

0.338 ± 0.163

7.065 ± 0.441

7.967 ± 0.681

3.157 ± 0.401

10.071 ± 0.713

2.292 ± 0.656

4.848 ± 0.347

3.457 ± 0.603

7.065 ± 0.565

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.992 ± 0.412

3.382 ± 0.625

4.66 ± 0.447

2.518 ± 0.289

6.05 ± 0.771

7.14 ± 0.546

6.84 ± 0.858

7.441 ± 0.408

2.029 ± 0.162

2.894 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski