Taibaiella chishuiensis
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4946 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P8D3W8|A0A2P8D3W8_9BACT Glutamine--fructose-6-phosphate aminotransferase [isomerizing] OS=Taibaiella chishuiensis OX=1434707 GN=glmS PE=3 SV=1
MM1 pKa = 8.45 DD2 pKa = 3.4 YY3 pKa = 10.69 TKK5 pKa = 10.62 AIRR8 pKa = 11.84 QLYY11 pKa = 9.13 DD12 pKa = 3.09 LPGGQRR18 pKa = 11.84 YY19 pKa = 8.67 ALPEE23 pKa = 3.87 QEE25 pKa = 4.52 LAALEE30 pKa = 4.03 ARR32 pKa = 11.84 IGRR35 pKa = 11.84 PLPALLLDD43 pKa = 3.74 YY44 pKa = 11.16 YY45 pKa = 10.87 RR46 pKa = 11.84 DD47 pKa = 3.68 LGAHH51 pKa = 6.0 EE52 pKa = 4.73 NLNNTHH58 pKa = 6.4 NCLLQPGEE66 pKa = 4.39 IVLSAAGYY74 pKa = 9.61 LVFYY78 pKa = 10.09 EE79 pKa = 4.28 EE80 pKa = 4.32 NQGVVQWAIKK90 pKa = 8.67 QTDD93 pKa = 4.03 LQLADD98 pKa = 3.59 PPVWGNYY105 pKa = 8.6 GSEE108 pKa = 4.1 EE109 pKa = 4.15 TPDD112 pKa = 3.29 WVLEE116 pKa = 4.15 SAALSDD122 pKa = 4.22 FWLLMAVYY130 pKa = 9.24 NGTMGALPYY139 pKa = 9.8 NANAFGPLDD148 pKa = 3.52 QAVATVVAQNWTAVPAISWDD168 pKa = 3.41 KK169 pKa = 10.44 QKK171 pKa = 11.28 VYY173 pKa = 10.4 TRR175 pKa = 11.84 DD176 pKa = 3.37 FRR178 pKa = 11.84 EE179 pKa = 4.33 VISLSFDD186 pKa = 3.76 DD187 pKa = 5.59 EE188 pKa = 4.89 GACTAIFACTADD200 pKa = 3.52 QEE202 pKa = 4.82 RR203 pKa = 11.84 FDD205 pKa = 4.97 ALMDD209 pKa = 4.13 KK210 pKa = 11.14 LPVDD214 pKa = 4.0 WDD216 pKa = 3.71 YY217 pKa = 11.98 CSYY220 pKa = 11.82 DD221 pKa = 3.48 DD222 pKa = 4.39 MEE224 pKa = 4.66 EE225 pKa = 4.16 EE226 pKa = 4.45 EE227 pKa = 5.07 EE228 pKa = 4.2 EE229 pKa = 4.82 DD230 pKa = 4.94 DD231 pKa = 4.3 VV232 pKa = 5.12
Molecular weight: 26.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.656
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.757
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.164
Thurlkill 3.694
EMBOSS 3.77
Sillero 3.973
Patrickios 0.718
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.878
Protein with the highest isoelectric point:
>tr|A0A2P8D447|A0A2P8D447_9BACT Uncharacterized protein OS=Taibaiella chishuiensis OX=1434707 GN=B0I18_10481 PE=4 SV=1
MM1 pKa = 7.69 PKK3 pKa = 10.34 VKK5 pKa = 8.9 THH7 pKa = 5.39 SRR9 pKa = 11.84 AKK11 pKa = 9.54 KK12 pKa = 7.25 TFKK15 pKa = 10.04 VTGTGKK21 pKa = 9.87 ISRR24 pKa = 11.84 RR25 pKa = 11.84 KK26 pKa = 9.83 AFRR29 pKa = 11.84 KK30 pKa = 9.62 HH31 pKa = 6.25 LLTKK35 pKa = 10.28 KK36 pKa = 8.91 SASRR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 SLDD45 pKa = 2.79 GSVFVHH51 pKa = 6.33 SANLPLVKK59 pKa = 10.37 RR60 pKa = 11.84 MLNMRR65 pKa = 4.51
Molecular weight: 7.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.385
IPC2_protein 10.847
IPC_protein 12.384
Toseland 12.574
ProMoST 13.056
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.457
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.179
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.016
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4946
0
4946
1698191
29
6178
343.3
38.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.73 ± 0.043
1.027 ± 0.015
5.225 ± 0.025
5.186 ± 0.042
4.559 ± 0.027
7.33 ± 0.04
1.947 ± 0.016
6.317 ± 0.031
5.578 ± 0.04
9.727 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.358 ± 0.018
4.991 ± 0.042
4.263 ± 0.023
4.176 ± 0.028
4.66 ± 0.029
6.005 ± 0.032
6.181 ± 0.054
6.354 ± 0.027
1.188 ± 0.012
4.198 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here