Gambusia affinis (Western mosquitofish) (Heterandria affinis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19443 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A315V7N6|A0A315V7N6_GAMAF Uncharacterized protein OS=Gambusia affinis OX=33528 GN=CCH79_00018424 PE=4 SV=1
MM1 pKa = 8.08DD2 pKa = 5.43APSQVWMGDD11 pKa = 3.54PTSGPSTSGPLATPAQADD29 pKa = 3.74SLIIEE34 pKa = 4.8PGSSISVSFTVSSVEE49 pKa = 3.77TGTFIIQASNDD60 pKa = 3.08RR61 pKa = 11.84DD62 pKa = 3.36FAMVQTPSVTIGAGDD77 pKa = 4.15GGQASDD83 pKa = 4.07MVVLIAPASAASGTDD98 pKa = 3.19VSLTIVAEE106 pKa = 4.22NADD109 pKa = 4.27GTEE112 pKa = 3.8MNYY115 pKa = 9.96VVLRR119 pKa = 11.84FSVAIKK125 pKa = 9.63VTDD128 pKa = 3.61HH129 pKa = 6.87SSPVCQVDD137 pKa = 3.68RR138 pKa = 11.84MSGVCPASPLLCASVQWEE156 pKa = 4.2LVVNFTDD163 pKa = 5.67GINGTGIEE171 pKa = 4.57TISLRR176 pKa = 11.84QGNGTFSTSNVVGVGGEE193 pKa = 4.13NITVVSYY200 pKa = 11.53SSTCCSQSVQLAAVDD215 pKa = 3.64RR216 pKa = 11.84VGNVGICTEE225 pKa = 4.14TAITLTTTAPSSTTSLVTNINHH247 pKa = 6.02ITTTIPP253 pKa = 2.99

Molecular weight: 25.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A315V7Z1|A0A315V7Z1_GAMAF Uncharacterized protein (Fragment) OS=Gambusia affinis OX=33528 GN=CCH79_00007011 PE=3 SV=1
MM1 pKa = 7.22MIIRR5 pKa = 11.84GTQTLLLVLVAWKK18 pKa = 10.55LFLIRR23 pKa = 11.84SKK25 pKa = 10.79LRR27 pKa = 11.84QLKK30 pKa = 9.95LHH32 pKa = 6.42LLHH35 pKa = 7.25RR36 pKa = 11.84IMNGRR41 pKa = 11.84SSGSWRR47 pKa = 11.84KK48 pKa = 9.31

Molecular weight: 5.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19443

0

19443

11554075

12

11644

594.3

66.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.624 ± 0.017

2.302 ± 0.012

5.085 ± 0.013

6.823 ± 0.019

3.632 ± 0.012

6.305 ± 0.019

2.653 ± 0.01

4.206 ± 0.014

5.54 ± 0.019

9.652 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.007

3.776 ± 0.01

5.786 ± 0.019

4.766 ± 0.015

5.902 ± 0.015

9.053 ± 0.022

5.57 ± 0.012

6.342 ± 0.015

1.185 ± 0.005

2.518 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski