Oat chlorotic stunt virus (isolate United Kingdom) (OCSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Avenavirus; Oat chlorotic stunt virus

Average proteome isoelectric point is 8.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|Q83929|P8_OCSVU Uncharacterized protein p8 OS=Oat chlorotic stunt virus (isolate United Kingdom) OX=652110 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84WLSRR6 pKa = 11.84TVKK9 pKa = 10.16CWVPLILAPLHH20 pKa = 6.38RR21 pKa = 11.84MSPLSVALATEE32 pKa = 5.51LILGCQLSSLGCLQLPLITRR52 pKa = 11.84STNFVGSASLLSRR65 pKa = 11.84WFLPIIAEE73 pKa = 4.19EE74 pKa = 4.04

Molecular weight:
8.22 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|Q83928|CAPSD_OCSVU Capsid protein OS=Oat chlorotic stunt virus (isolate United Kingdom) OX=652110 GN=ORF2 PE=3 SV=1
MM1 pKa = 7.49SWPSLSGVPNGSSSVSTWLSVTHH24 pKa = 6.37PRR26 pKa = 11.84RR27 pKa = 11.84SLPKK31 pKa = 9.65EE32 pKa = 3.69LLTAFDD38 pKa = 3.97TCNVAPEE45 pKa = 4.16ALLVLRR51 pKa = 11.84STSLMILEE59 pKa = 4.38EE60 pKa = 4.24TCVVVGAAEE69 pKa = 4.13MPTAEE74 pKa = 4.62DD75 pKa = 3.31NSGRR79 pKa = 11.84EE80 pKa = 4.1LFIGSNGDD88 pKa = 3.08PMEE91 pKa = 4.67RR92 pKa = 11.84KK93 pKa = 7.71TRR95 pKa = 11.84TAHH98 pKa = 5.87HH99 pKa = 7.14AIKK102 pKa = 9.36KK103 pKa = 5.84TVRR106 pKa = 11.84IKK108 pKa = 10.48KK109 pKa = 7.85GHH111 pKa = 5.79RR112 pKa = 11.84TTFAMTVANGAYY124 pKa = 9.69VKK126 pKa = 10.69FGARR130 pKa = 11.84PLTEE134 pKa = 4.45ANVLVVRR141 pKa = 11.84KK142 pKa = 9.44WIVKK146 pKa = 9.94LIADD150 pKa = 4.41EE151 pKa = 4.33YY152 pKa = 11.2KK153 pKa = 10.61DD154 pKa = 5.61LRR156 pKa = 11.84VCDD159 pKa = 3.51QALVIDD165 pKa = 4.49RR166 pKa = 11.84ATFLSFIPTMAWNNYY181 pKa = 8.53KK182 pKa = 10.66FIFHH186 pKa = 6.98GKK188 pKa = 8.61NAVTDD193 pKa = 4.26RR194 pKa = 11.84VAGEE198 pKa = 4.06NLFSRR203 pKa = 11.84IAQWANPGKK212 pKa = 7.85XGCPVVVTGQGCVISRR228 pKa = 11.84APDD231 pKa = 3.54CAQLRR236 pKa = 11.84VKK238 pKa = 10.59RR239 pKa = 11.84LLGVTKK245 pKa = 10.48NRR247 pKa = 11.84TCMRR251 pKa = 11.84VSGVSPNIQIIPFNNDD267 pKa = 1.5ITTLEE272 pKa = 3.95RR273 pKa = 11.84AIKK276 pKa = 10.1EE277 pKa = 3.67RR278 pKa = 11.84VFFVKK283 pKa = 10.52NLDD286 pKa = 3.47KK287 pKa = 11.15GSPTKK292 pKa = 10.3FVSPPRR298 pKa = 11.84PAPGVFAQRR307 pKa = 11.84LSNTLGLLVPFLPSTAPMSHH327 pKa = 5.09QQFVDD332 pKa = 3.51STPSRR337 pKa = 11.84KK338 pKa = 9.59RR339 pKa = 11.84KK340 pKa = 9.58VYY342 pKa = 9.88QQALEE347 pKa = 5.08DD348 pKa = 4.04ISCHH352 pKa = 5.09GLNLEE357 pKa = 4.14TDD359 pKa = 3.77SKK361 pKa = 11.55VKK363 pKa = 10.8VFVKK367 pKa = 10.33YY368 pKa = 10.48EE369 pKa = 4.19KK370 pKa = 9.81TDD372 pKa = 3.44HH373 pKa = 6.22TSKK376 pKa = 10.94ADD378 pKa = 3.39PVPRR382 pKa = 11.84VISPRR387 pKa = 11.84DD388 pKa = 3.33PKK390 pKa = 11.46YY391 pKa = 10.65NLALGRR397 pKa = 11.84YY398 pKa = 6.96LRR400 pKa = 11.84PMEE403 pKa = 3.9EE404 pKa = 4.57RR405 pKa = 11.84IFKK408 pKa = 10.79ALGKK412 pKa = 10.23LFGHH416 pKa = 5.7RR417 pKa = 11.84TVMKK421 pKa = 10.77GMDD424 pKa = 3.57TDD426 pKa = 3.39VTARR430 pKa = 11.84VIQEE434 pKa = 3.64KK435 pKa = 10.16WNMFNKK441 pKa = 9.85PVAIGLDD448 pKa = 3.26ASRR451 pKa = 11.84FDD453 pKa = 3.31QHH455 pKa = 7.58VSLEE459 pKa = 4.06ALEE462 pKa = 4.92FEE464 pKa = 4.21HH465 pKa = 7.49SVYY468 pKa = 10.45LKK470 pKa = 10.21CVRR473 pKa = 11.84RR474 pKa = 11.84MVDD477 pKa = 2.93KK478 pKa = 11.0RR479 pKa = 11.84KK480 pKa = 10.14LGNILRR486 pKa = 11.84HH487 pKa = 5.24QLLNKK492 pKa = 10.3CYY494 pKa = 10.78GNTPDD499 pKa = 4.16GAVSYY504 pKa = 9.09TIEE507 pKa = 4.19GTRR510 pKa = 11.84MSGDD514 pKa = 3.41MNTSLGNCVLMCMMIHH530 pKa = 7.54AYY532 pKa = 9.94GLHH535 pKa = 6.25KK536 pKa = 10.63SVNIQLANNGDD547 pKa = 3.8DD548 pKa = 3.28CVVFLEE554 pKa = 4.22QSDD557 pKa = 4.08LATFSEE563 pKa = 5.53GLFEE567 pKa = 4.6WFLEE571 pKa = 4.15MGFNMAIEE579 pKa = 4.37EE580 pKa = 4.0PSYY583 pKa = 10.47EE584 pKa = 3.98LEE586 pKa = 4.77HH587 pKa = 8.84IEE589 pKa = 4.13FCQCRR594 pKa = 11.84PVFDD598 pKa = 3.85GVKK601 pKa = 9.18YY602 pKa = 8.6TMCRR606 pKa = 11.84NPRR609 pKa = 11.84TAIAKK614 pKa = 9.78DD615 pKa = 3.53SVYY618 pKa = 10.64LKK620 pKa = 10.75HH621 pKa = 6.66VDD623 pKa = 3.41QFVTYY628 pKa = 10.05SSWLNAVGTGGLALAGGLPIFDD650 pKa = 5.14AFYY653 pKa = 10.05TCYY656 pKa = 10.39KK657 pKa = 10.17RR658 pKa = 11.84NSNSHH663 pKa = 5.22WFSGRR668 pKa = 11.84KK669 pKa = 9.2GRR671 pKa = 11.84LKK673 pKa = 9.97TLSSVDD679 pKa = 4.78DD680 pKa = 3.78SLPWFMRR687 pKa = 11.84EE688 pKa = 4.12LGLKK692 pKa = 9.72GKK694 pKa = 10.25RR695 pKa = 11.84SSAEE699 pKa = 3.61PLPASRR705 pKa = 11.84ASFYY709 pKa = 10.74LAWGVTPCEE718 pKa = 3.68QLEE721 pKa = 4.18LEE723 pKa = 4.61KK724 pKa = 10.86YY725 pKa = 9.31YY726 pKa = 11.11KK727 pKa = 10.29SFKK730 pKa = 10.61LDD732 pKa = 3.03TSTLLEE738 pKa = 3.81EE739 pKa = 5.48HH740 pKa = 6.77LWQPRR745 pKa = 11.84GVFPDD750 pKa = 3.38EE751 pKa = 4.4DD752 pKa = 3.52

Molecular weight:
84.34 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1280

74

752

426.7

46.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.187 ± 0.489

2.344 ± 0.246

3.828 ± 0.846

4.375 ± 0.811

4.297 ± 0.523

7.109 ± 0.848

2.109 ± 0.239

4.297 ± 0.428

5.391 ± 0.879

9.844 ± 1.519

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.656 ± 0.237

4.688 ± 0.509

5.391 ± 0.176

2.344 ± 0.179

5.391 ± 0.934

8.281 ± 0.95

7.734 ± 1.529

8.438 ± 0.56

1.406 ± 0.433

2.813 ± 0.435

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski