Oat chlorotic stunt virus (isolate United Kingdom) (OCSV)
Average proteome isoelectric point is 8.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q83929|P8_OCSVU Uncharacterized protein p8 OS=Oat chlorotic stunt virus (isolate United Kingdom) OX=652110 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.68 RR2 pKa = 11.84 WLSRR6 pKa = 11.84 TVKK9 pKa = 10.16 CWVPLILAPLHH20 pKa = 6.38 RR21 pKa = 11.84 MSPLSVALATEE32 pKa = 5.51 LILGCQLSSLGCLQLPLITRR52 pKa = 11.84 STNFVGSASLLSRR65 pKa = 11.84 WFLPIIAEE73 pKa = 4.19 EE74 pKa = 4.04
Molecular weight: 8.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.321
IPC2_protein 8.126
IPC_protein 8.419
Toseland 8.083
ProMoST 8.551
Dawson 8.741
Bjellqvist 9.209
Wikipedia 8.653
Rodwell 8.726
Grimsley 7.644
Solomon 9.194
Lehninger 9.253
Nozaki 9.692
DTASelect 8.843
Thurlkill 8.902
EMBOSS 8.99
Sillero 9.297
Patrickios 7.585
IPC_peptide 9.194
IPC2_peptide 9.224
IPC2.peptide.svr19 8.812
Protein with the highest isoelectric point:
>sp|Q83928|CAPSD_OCSVU Capsid protein OS=Oat chlorotic stunt virus (isolate United Kingdom) OX=652110 GN=ORF2 PE=3 SV=1
MM1 pKa = 7.49 SWPSLSGVPNGSSSVSTWLSVTHH24 pKa = 6.37 PRR26 pKa = 11.84 RR27 pKa = 11.84 SLPKK31 pKa = 9.65 EE32 pKa = 3.69 LLTAFDD38 pKa = 3.97 TCNVAPEE45 pKa = 4.16 ALLVLRR51 pKa = 11.84 STSLMILEE59 pKa = 4.38 EE60 pKa = 4.24 TCVVVGAAEE69 pKa = 4.13 MPTAEE74 pKa = 4.62 DD75 pKa = 3.31 NSGRR79 pKa = 11.84 EE80 pKa = 4.1 LFIGSNGDD88 pKa = 3.08 PMEE91 pKa = 4.67 RR92 pKa = 11.84 KK93 pKa = 7.71 TRR95 pKa = 11.84 TAHH98 pKa = 5.87 HH99 pKa = 7.14 AIKK102 pKa = 9.36 KK103 pKa = 5.84 TVRR106 pKa = 11.84 IKK108 pKa = 10.48 KK109 pKa = 7.85 GHH111 pKa = 5.79 RR112 pKa = 11.84 TTFAMTVANGAYY124 pKa = 9.69 VKK126 pKa = 10.69 FGARR130 pKa = 11.84 PLTEE134 pKa = 4.45 ANVLVVRR141 pKa = 11.84 KK142 pKa = 9.44 WIVKK146 pKa = 9.94 LIADD150 pKa = 4.41 EE151 pKa = 4.33 YY152 pKa = 11.2 KK153 pKa = 10.61 DD154 pKa = 5.61 LRR156 pKa = 11.84 VCDD159 pKa = 3.51 QALVIDD165 pKa = 4.49 RR166 pKa = 11.84 ATFLSFIPTMAWNNYY181 pKa = 8.53 KK182 pKa = 10.66 FIFHH186 pKa = 6.98 GKK188 pKa = 8.61 NAVTDD193 pKa = 4.26 RR194 pKa = 11.84 VAGEE198 pKa = 4.06 NLFSRR203 pKa = 11.84 IAQWANPGKK212 pKa = 7.85 XGCPVVVTGQGCVISRR228 pKa = 11.84 APDD231 pKa = 3.54 CAQLRR236 pKa = 11.84 VKK238 pKa = 10.59 RR239 pKa = 11.84 LLGVTKK245 pKa = 10.48 NRR247 pKa = 11.84 TCMRR251 pKa = 11.84 VSGVSPNIQIIPFNNDD267 pKa = 1.5 ITTLEE272 pKa = 3.95 RR273 pKa = 11.84 AIKK276 pKa = 10.1 EE277 pKa = 3.67 RR278 pKa = 11.84 VFFVKK283 pKa = 10.52 NLDD286 pKa = 3.47 KK287 pKa = 11.15 GSPTKK292 pKa = 10.3 FVSPPRR298 pKa = 11.84 PAPGVFAQRR307 pKa = 11.84 LSNTLGLLVPFLPSTAPMSHH327 pKa = 5.09 QQFVDD332 pKa = 3.51 STPSRR337 pKa = 11.84 KK338 pKa = 9.59 RR339 pKa = 11.84 KK340 pKa = 9.58 VYY342 pKa = 9.88 QQALEE347 pKa = 5.08 DD348 pKa = 4.04 ISCHH352 pKa = 5.09 GLNLEE357 pKa = 4.14 TDD359 pKa = 3.77 SKK361 pKa = 11.55 VKK363 pKa = 10.8 VFVKK367 pKa = 10.33 YY368 pKa = 10.48 EE369 pKa = 4.19 KK370 pKa = 9.81 TDD372 pKa = 3.44 HH373 pKa = 6.22 TSKK376 pKa = 10.94 ADD378 pKa = 3.39 PVPRR382 pKa = 11.84 VISPRR387 pKa = 11.84 DD388 pKa = 3.33 PKK390 pKa = 11.46 YY391 pKa = 10.65 NLALGRR397 pKa = 11.84 YY398 pKa = 6.96 LRR400 pKa = 11.84 PMEE403 pKa = 3.9 EE404 pKa = 4.57 RR405 pKa = 11.84 IFKK408 pKa = 10.79 ALGKK412 pKa = 10.23 LFGHH416 pKa = 5.7 RR417 pKa = 11.84 TVMKK421 pKa = 10.77 GMDD424 pKa = 3.57 TDD426 pKa = 3.39 VTARR430 pKa = 11.84 VIQEE434 pKa = 3.64 KK435 pKa = 10.16 WNMFNKK441 pKa = 9.85 PVAIGLDD448 pKa = 3.26 ASRR451 pKa = 11.84 FDD453 pKa = 3.31 QHH455 pKa = 7.58 VSLEE459 pKa = 4.06 ALEE462 pKa = 4.92 FEE464 pKa = 4.21 HH465 pKa = 7.49 SVYY468 pKa = 10.45 LKK470 pKa = 10.21 CVRR473 pKa = 11.84 RR474 pKa = 11.84 MVDD477 pKa = 2.93 KK478 pKa = 11.0 RR479 pKa = 11.84 KK480 pKa = 10.14 LGNILRR486 pKa = 11.84 HH487 pKa = 5.24 QLLNKK492 pKa = 10.3 CYY494 pKa = 10.78 GNTPDD499 pKa = 4.16 GAVSYY504 pKa = 9.09 TIEE507 pKa = 4.19 GTRR510 pKa = 11.84 MSGDD514 pKa = 3.41 MNTSLGNCVLMCMMIHH530 pKa = 7.54 AYY532 pKa = 9.94 GLHH535 pKa = 6.25 KK536 pKa = 10.63 SVNIQLANNGDD547 pKa = 3.8 DD548 pKa = 3.28 CVVFLEE554 pKa = 4.22 QSDD557 pKa = 4.08 LATFSEE563 pKa = 5.53 GLFEE567 pKa = 4.6 WFLEE571 pKa = 4.15 MGFNMAIEE579 pKa = 4.37 EE580 pKa = 4.0 PSYY583 pKa = 10.47 EE584 pKa = 3.98 LEE586 pKa = 4.77 HH587 pKa = 8.84 IEE589 pKa = 4.13 FCQCRR594 pKa = 11.84 PVFDD598 pKa = 3.85 GVKK601 pKa = 9.18 YY602 pKa = 8.6 TMCRR606 pKa = 11.84 NPRR609 pKa = 11.84 TAIAKK614 pKa = 9.78 DD615 pKa = 3.53 SVYY618 pKa = 10.64 LKK620 pKa = 10.75 HH621 pKa = 6.66 VDD623 pKa = 3.41 QFVTYY628 pKa = 10.05 SSWLNAVGTGGLALAGGLPIFDD650 pKa = 5.14 AFYY653 pKa = 10.05 TCYY656 pKa = 10.39 KK657 pKa = 10.17 RR658 pKa = 11.84 NSNSHH663 pKa = 5.22 WFSGRR668 pKa = 11.84 KK669 pKa = 9.2 GRR671 pKa = 11.84 LKK673 pKa = 9.97 TLSSVDD679 pKa = 4.78 DD680 pKa = 3.78 SLPWFMRR687 pKa = 11.84 EE688 pKa = 4.12 LGLKK692 pKa = 9.72 GKK694 pKa = 10.25 RR695 pKa = 11.84 SSAEE699 pKa = 3.61 PLPASRR705 pKa = 11.84 ASFYY709 pKa = 10.74 LAWGVTPCEE718 pKa = 3.68 QLEE721 pKa = 4.18 LEE723 pKa = 4.61 KK724 pKa = 10.86 YY725 pKa = 9.31 YY726 pKa = 11.11 KK727 pKa = 10.29 SFKK730 pKa = 10.61 LDD732 pKa = 3.03 TSTLLEE738 pKa = 3.81 EE739 pKa = 5.48 HH740 pKa = 6.77 LWQPRR745 pKa = 11.84 GVFPDD750 pKa = 3.38 EE751 pKa = 4.4 DD752 pKa = 3.52
Molecular weight: 84.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.411
IPC2_protein 8.331
IPC_protein 8.156
Toseland 8.521
ProMoST 8.756
Dawson 9.092
Bjellqvist 9.151
Wikipedia 9.151
Rodwell 9.238
Grimsley 8.756
Solomon 9.136
Lehninger 9.121
Nozaki 9.355
DTASelect 8.96
Thurlkill 9.077
EMBOSS 9.253
Sillero 9.311
Patrickios 4.711
IPC_peptide 9.136
IPC2_peptide 7.951
IPC2.peptide.svr19 7.798
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1280
74
752
426.7
46.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.187 ± 0.489
2.344 ± 0.246
3.828 ± 0.846
4.375 ± 0.811
4.297 ± 0.523
7.109 ± 0.848
2.109 ± 0.239
4.297 ± 0.428
5.391 ± 0.879
9.844 ± 1.519
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.656 ± 0.237
4.688 ± 0.509
5.391 ± 0.176
2.344 ± 0.179
5.391 ± 0.934
8.281 ± 0.95
7.734 ± 1.529
8.438 ± 0.56
1.406 ± 0.433
2.813 ± 0.435
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here