Pseudomonas phage MR299-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Bruynoghevirus; unclassified Bruynoghevirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H8ZM39|H8ZM39_9CAUD Uncharacterized protein OS=Pseudomonas phage MR299-2 OX=1158721 GN=I7C_038c PE=4 SV=1
MM1 pKa = 7.82DD2 pKa = 4.91NKK4 pKa = 10.58YY5 pKa = 10.82KK6 pKa = 9.28PTPQDD11 pKa = 3.17EE12 pKa = 4.36ARR14 pKa = 11.84KK15 pKa = 8.76EE16 pKa = 4.03AALEE20 pKa = 3.94AAFSGGWGGPEE31 pKa = 3.25VDD33 pKa = 4.94ADD35 pKa = 4.42DD36 pKa = 5.3FEE38 pKa = 6.5LGNACGLDD46 pKa = 3.55PEE48 pKa = 4.78VCEE51 pKa = 4.52SCQQ54 pKa = 3.52

Molecular weight: 5.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H8ZM67|H8ZM67_9CAUD Uncharacterized protein OS=Pseudomonas phage MR299-2 OX=1158721 GN=I7C_066c PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.34SPYY5 pKa = 9.36EE6 pKa = 4.1AAHH9 pKa = 6.38EE10 pKa = 3.86RR11 pKa = 11.84AQMINRR17 pKa = 11.84LKK19 pKa = 10.78KK20 pKa = 7.41LTRR23 pKa = 11.84MIRR26 pKa = 11.84VHH28 pKa = 7.21PDD30 pKa = 2.8PRR32 pKa = 11.84WIVEE36 pKa = 3.95RR37 pKa = 11.84QEE39 pKa = 4.88LIRR42 pKa = 11.84KK43 pKa = 8.89LSKK46 pKa = 10.75

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13765

26

1056

202.4

22.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.282 ± 0.572

1.024 ± 0.146

6.168 ± 0.242

7.127 ± 0.403

3.923 ± 0.211

8.267 ± 0.35

2.027 ± 0.189

5.274 ± 0.194

5.55 ± 0.33

8.347 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.826 ± 0.199

4.366 ± 0.246

4.228 ± 0.243

3.879 ± 0.276

5.848 ± 0.252

5.717 ± 0.238

5.165 ± 0.264

6.996 ± 0.267

1.656 ± 0.156

3.327 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski