Paenibacillus yonginensis
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4173 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B1N1N7|A0A1B1N1N7_9BACL SAF domain-containing protein OS=Paenibacillus yonginensis OX=1462996 GN=AWM70_12655 PE=4 SV=1
MM1 pKa = 7.08 NWEE4 pKa = 3.74 GAYY7 pKa = 10.5 GKK9 pKa = 8.61 TYY11 pKa = 10.1 QVQVSQAEE19 pKa = 4.13 QPGDD23 pKa = 4.11 DD24 pKa = 5.65 DD25 pKa = 3.47 WTDD28 pKa = 2.98 WYY30 pKa = 10.21 TEE32 pKa = 3.8 NAGNGGQDD40 pKa = 4.54 LIFEE44 pKa = 4.58 EE45 pKa = 4.65 PAEE48 pKa = 3.97 ARR50 pKa = 11.84 YY51 pKa = 10.26 VRR53 pKa = 11.84 ILGTARR59 pKa = 11.84 GTKK62 pKa = 9.79 YY63 pKa = 10.68 GYY65 pKa = 9.28 SLWGMEE71 pKa = 4.72 VYY73 pKa = 8.01 GTPAGDD79 pKa = 3.43 SGEE82 pKa = 4.22 TGEE85 pKa = 6.06 DD86 pKa = 3.49 GDD88 pKa = 4.41 NPGSGTADD96 pKa = 3.46 PLAPPTLTADD106 pKa = 3.51 TYY108 pKa = 11.91 DD109 pKa = 3.47 NYY111 pKa = 11.67 ADD113 pKa = 3.86 RR114 pKa = 11.84 TT115 pKa = 3.98
Molecular weight: 12.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.719
IPC_protein 3.681
Toseland 3.478
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.389
Solomon 3.656
Lehninger 3.617
Nozaki 3.808
DTASelect 4.012
Thurlkill 3.541
EMBOSS 3.63
Sillero 3.808
Patrickios 0.54
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.73
Protein with the highest isoelectric point:
>tr|A0A1B1N4S3|A0A1B1N4S3_9BACL Uncharacterized protein OS=Paenibacillus yonginensis OX=1462996 GN=AWM70_18990 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 8.7 PTFKK6 pKa = 10.81 PNVSKK11 pKa = 10.8 RR12 pKa = 11.84 KK13 pKa = 8.95 KK14 pKa = 8.25 VHH16 pKa = 5.49 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTKK25 pKa = 10.18 NGRR28 pKa = 11.84 KK29 pKa = 8.87 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.2 GRR39 pKa = 11.84 KK40 pKa = 8.79 VLSAA44 pKa = 4.05
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.261
Rodwell 12.705
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.793
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.427
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.035
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4173
0
4173
1336961
26
1897
320.4
35.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.599 ± 0.049
0.713 ± 0.01
5.036 ± 0.029
6.981 ± 0.052
4.045 ± 0.029
7.759 ± 0.037
1.993 ± 0.018
6.294 ± 0.035
5.283 ± 0.037
10.365 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.683 ± 0.018
3.654 ± 0.027
4.165 ± 0.025
4.037 ± 0.03
5.199 ± 0.036
6.296 ± 0.035
5.216 ± 0.029
7.017 ± 0.029
1.246 ± 0.015
3.421 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here