Vernonia crinkle betasatellite
Average proteome isoelectric point is 4.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L4AB17|A0A1L4AB17_9VIRU BetaC1 protein OS=Vernonia crinkle betasatellite OX=1925152 GN=betaC1 PE=4 SV=1
MM1 pKa = 7.56 PHH3 pKa = 6.39 TATTLTMIKK12 pKa = 9.28 FTTNTTLPSPNICIIKK28 pKa = 10.58 LEE30 pKa = 4.14 ITSANQPYY38 pKa = 9.93 LVKK41 pKa = 10.54 HH42 pKa = 6.57 KK43 pKa = 10.64 ISIPYY48 pKa = 8.53 TYY50 pKa = 10.13 RR51 pKa = 11.84 DD52 pKa = 3.51 IITPFDD58 pKa = 3.62 LQGNDD63 pKa = 3.49 TLISLALRR71 pKa = 11.84 FAFNAKK77 pKa = 9.95 EE78 pKa = 3.86 YY79 pKa = 11.14 GEE81 pKa = 4.99 LKK83 pKa = 10.38 IEE85 pKa = 5.13 DD86 pKa = 4.11 IIEE89 pKa = 4.28 LVDD92 pKa = 5.6 LMVLQNDD99 pKa = 3.92 SFVDD103 pKa = 3.3 IDD105 pKa = 4.05 VEE107 pKa = 3.94 ASYY110 pKa = 10.9 TNRR113 pKa = 11.84 RR114 pKa = 11.84 TITQQ118 pKa = 3.21
Molecular weight: 13.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.905
IPC2_protein 5.029
IPC_protein 4.863
Toseland 4.762
ProMoST 5.003
Dawson 4.851
Bjellqvist 4.991
Wikipedia 4.749
Rodwell 4.749
Grimsley 4.673
Solomon 4.851
Lehninger 4.813
Nozaki 4.978
DTASelect 5.156
Thurlkill 4.774
EMBOSS 4.774
Sillero 5.029
Patrickios 3.897
IPC_peptide 4.851
IPC2_peptide 5.016
IPC2.peptide.svr19 5.037
Protein with the highest isoelectric point:
>tr|A0A1L4AB17|A0A1L4AB17_9VIRU BetaC1 protein OS=Vernonia crinkle betasatellite OX=1925152 GN=betaC1 PE=4 SV=1
MM1 pKa = 7.56 PHH3 pKa = 6.39 TATTLTMIKK12 pKa = 9.28 FTTNTTLPSPNICIIKK28 pKa = 10.58 LEE30 pKa = 4.14 ITSANQPYY38 pKa = 9.93 LVKK41 pKa = 10.54 HH42 pKa = 6.57 KK43 pKa = 10.64 ISIPYY48 pKa = 8.53 TYY50 pKa = 10.13 RR51 pKa = 11.84 DD52 pKa = 3.51 IITPFDD58 pKa = 3.62 LQGNDD63 pKa = 3.49 TLISLALRR71 pKa = 11.84 FAFNAKK77 pKa = 9.95 EE78 pKa = 3.86 YY79 pKa = 11.14 GEE81 pKa = 4.99 LKK83 pKa = 10.38 IEE85 pKa = 5.13 DD86 pKa = 4.11 IIEE89 pKa = 4.28 LVDD92 pKa = 5.6 LMVLQNDD99 pKa = 3.92 SFVDD103 pKa = 3.3 IDD105 pKa = 4.05 VEE107 pKa = 3.94 ASYY110 pKa = 10.9 TNRR113 pKa = 11.84 RR114 pKa = 11.84 TITQQ118 pKa = 3.21
Molecular weight: 13.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.905
IPC2_protein 5.029
IPC_protein 4.863
Toseland 4.762
ProMoST 5.003
Dawson 4.851
Bjellqvist 4.991
Wikipedia 4.749
Rodwell 4.749
Grimsley 4.673
Solomon 4.851
Lehninger 4.813
Nozaki 4.978
DTASelect 5.156
Thurlkill 4.774
EMBOSS 4.774
Sillero 5.029
Patrickios 3.897
IPC_peptide 4.851
IPC2_peptide 5.016
IPC2.peptide.svr19 5.037
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
118
118
118
118.0
13.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.085 ± 0.0
0.847 ± 0.0
6.78 ± 0.0
5.085 ± 0.0
4.237 ± 0.0
1.695 ± 0.0
1.695 ± 0.0
12.712 ± 0.0
5.085 ± 0.0
10.169 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.542 ± 0.0
5.932 ± 0.0
5.085 ± 0.0
3.39 ± 0.0
3.39 ± 0.0
5.085 ± 0.0
12.712 ± 0.0
4.237 ± 0.0
0.0 ± 0.0
4.237 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here