Kocuria sp. WRN011

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Kocuria; unclassified Kocuria

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2905 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A3AGW7|A0A2A3AGW7_9MICC Uncharacterized protein OS=Kocuria sp. WRN011 OX=2029858 GN=CKW39_05680 PE=4 SV=1
MM1 pKa = 7.08RR2 pKa = 11.84TALIIVDD9 pKa = 3.91VQNDD13 pKa = 3.63FCEE16 pKa = 4.89GGSLAVTGGAAVAAQISEE34 pKa = 4.21FLEE37 pKa = 4.37DD38 pKa = 3.25HH39 pKa = 6.05HH40 pKa = 7.12TDD42 pKa = 3.75YY43 pKa = 11.33QVVAATQDD51 pKa = 3.06WHH53 pKa = 7.02IDD55 pKa = 3.67PGSHH59 pKa = 7.33FSDD62 pKa = 4.66DD63 pKa = 3.82PDD65 pKa = 4.49FVSSWPVHH73 pKa = 5.68CVAEE77 pKa = 4.36TEE79 pKa = 4.37GAEE82 pKa = 4.08THH84 pKa = 7.11SNFDD88 pKa = 3.08TDD90 pKa = 3.77YY91 pKa = 8.95VTEE94 pKa = 4.64FFRR97 pKa = 11.84KK98 pKa = 8.88GAYY101 pKa = 9.18DD102 pKa = 3.23AAYY105 pKa = 10.55SGFEE109 pKa = 4.2GLSAPDD115 pKa = 2.99VDD117 pKa = 4.22VPMGEE122 pKa = 4.4DD123 pKa = 3.6PEE125 pKa = 5.38DD126 pKa = 4.12ADD128 pKa = 4.43EE129 pKa = 4.55EE130 pKa = 4.77RR131 pKa = 11.84ISLDD135 pKa = 3.25DD136 pKa = 3.58WLQEE140 pKa = 3.92HH141 pKa = 7.5DD142 pKa = 3.93IEE144 pKa = 5.34AVDD147 pKa = 4.12VVGLALDD154 pKa = 3.78YY155 pKa = 10.94CVQATAKK162 pKa = 10.31DD163 pKa = 3.76AVDD166 pKa = 3.21AGYY169 pKa = 7.5EE170 pKa = 4.22TRR172 pKa = 11.84VLLPLTAPVAKK183 pKa = 10.22EE184 pKa = 3.91SGEE187 pKa = 4.13GATAEE192 pKa = 4.13LADD195 pKa = 4.02AGVAVVEE202 pKa = 4.6HH203 pKa = 7.05LDD205 pKa = 3.3

Molecular weight: 21.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A3AHA8|A0A2A3AHA8_9MICC Uncharacterized protein OS=Kocuria sp. WRN011 OX=2029858 GN=CKW39_06485 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.09RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84KK16 pKa = 8.24HH17 pKa = 4.59GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILNARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.44GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2905

0

2905

959224

32

1927

330.2

35.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.617 ± 0.051

0.644 ± 0.011

6.058 ± 0.039

6.197 ± 0.048

3.108 ± 0.027

8.554 ± 0.043

2.283 ± 0.023

4.409 ± 0.031

2.721 ± 0.035

9.731 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.232 ± 0.017

2.596 ± 0.026

5.296 ± 0.03

3.443 ± 0.023

6.696 ± 0.045

6.049 ± 0.03

6.137 ± 0.03

8.597 ± 0.04

1.506 ± 0.022

2.128 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski