Amino acid dipepetide frequency for Mycobacterium phage Roy17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.092AlaAla: 18.092 ± 1.431
1.16AlaCys: 1.16 ± 0.279
7.469AlaAsp: 7.469 ± 0.519
7.701AlaGlu: 7.701 ± 0.809
2.32AlaPhe: 2.32 ± 0.414
10.716AlaGly: 10.716 ± 1.244
2.366AlaHis: 2.366 ± 0.38
4.314AlaIle: 4.314 ± 0.578
3.526AlaLys: 3.526 ± 0.466
10.438AlaLeu: 10.438 ± 1.025
3.247AlaMet: 3.247 ± 0.354
3.062AlaAsn: 3.062 ± 0.416
6.727AlaPro: 6.727 ± 0.486
4.036AlaGln: 4.036 ± 0.446
8.768AlaArg: 8.768 ± 0.759
5.567AlaSer: 5.567 ± 0.5
8.165AlaThr: 8.165 ± 0.562
9.232AlaVal: 9.232 ± 0.928
2.088AlaTrp: 2.088 ± 0.333
3.062AlaTyr: 3.062 ± 0.382
0.0AlaXaa: 0.0 ± 0.0
Cys
0.696CysAla: 0.696 ± 0.206
0.0CysCys: 0.0 ± 0.0
0.881CysAsp: 0.881 ± 0.222
0.696CysGlu: 0.696 ± 0.203
0.325CysPhe: 0.325 ± 0.11
1.392CysGly: 1.392 ± 0.25
0.325CysHis: 0.325 ± 0.114
0.325CysIle: 0.325 ± 0.141
0.232CysLys: 0.232 ± 0.102
0.603CysLeu: 0.603 ± 0.163
0.232CysMet: 0.232 ± 0.088
0.278CysAsn: 0.278 ± 0.112
0.835CysPro: 0.835 ± 0.248
0.278CysGln: 0.278 ± 0.111
0.835CysArg: 0.835 ± 0.256
0.557CysSer: 0.557 ± 0.173
0.557CysThr: 0.557 ± 0.171
0.789CysVal: 0.789 ± 0.198
0.232CysTrp: 0.232 ± 0.123
0.325CysTyr: 0.325 ± 0.121
0.0CysXaa: 0.0 ± 0.0
Asp
7.191AspAla: 7.191 ± 0.556
0.928AspCys: 0.928 ± 0.25
4.871AspAsp: 4.871 ± 0.702
4.825AspGlu: 4.825 ± 0.62
1.624AspPhe: 1.624 ± 0.246
6.031AspGly: 6.031 ± 0.651
1.206AspHis: 1.206 ± 0.28
3.201AspIle: 3.201 ± 0.377
1.995AspLys: 1.995 ± 0.342
5.66AspLeu: 5.66 ± 0.445
1.067AspMet: 1.067 ± 0.275
2.134AspAsn: 2.134 ± 0.304
5.242AspPro: 5.242 ± 0.539
1.531AspGln: 1.531 ± 0.258
4.314AspArg: 4.314 ± 0.58
2.876AspSer: 2.876 ± 0.364
3.758AspThr: 3.758 ± 0.422
3.943AspVal: 3.943 ± 0.348
1.16AspTrp: 1.16 ± 0.252
1.902AspTyr: 1.902 ± 0.254
0.0AspXaa: 0.0 ± 0.0
Glu
7.376GluAla: 7.376 ± 0.773
0.696GluCys: 0.696 ± 0.202
3.479GluAsp: 3.479 ± 0.551
2.134GluGlu: 2.134 ± 0.262
2.18GluPhe: 2.18 ± 0.31
4.361GluGly: 4.361 ± 0.536
1.206GluHis: 1.206 ± 0.222
3.85GluIle: 3.85 ± 0.524
1.716GluLys: 1.716 ± 0.285
6.309GluLeu: 6.309 ± 0.599
0.881GluMet: 0.881 ± 0.197
0.974GluAsn: 0.974 ± 0.202
3.015GluPro: 3.015 ± 0.517
2.459GluGln: 2.459 ± 0.296
4.407GluArg: 4.407 ± 0.465
1.809GluSer: 1.809 ± 0.335
3.665GluThr: 3.665 ± 0.411
5.799GluVal: 5.799 ± 0.615
0.974GluTrp: 0.974 ± 0.21
1.067GluTyr: 1.067 ± 0.2
0.0GluXaa: 0.0 ± 0.0
Phe
2.876PheAla: 2.876 ± 0.361
0.278PheCys: 0.278 ± 0.11
1.624PheAsp: 1.624 ± 0.277
1.345PheGlu: 1.345 ± 0.243
0.418PhePhe: 0.418 ± 0.135
2.923PheGly: 2.923 ± 0.498
0.464PheHis: 0.464 ± 0.142
0.649PheIle: 0.649 ± 0.173
1.113PheLys: 1.113 ± 0.217
1.438PheLeu: 1.438 ± 0.283
0.278PheMet: 0.278 ± 0.097
0.881PheAsn: 0.881 ± 0.226
1.299PhePro: 1.299 ± 0.243
0.603PheGln: 0.603 ± 0.155
1.438PheArg: 1.438 ± 0.285
1.206PheSer: 1.206 ± 0.242
1.485PheThr: 1.485 ± 0.224
1.485PheVal: 1.485 ± 0.201
0.325PheTrp: 0.325 ± 0.126
0.603PheTyr: 0.603 ± 0.16
0.0PheXaa: 0.0 ± 0.0
Gly
9.557GlyAla: 9.557 ± 1.557
1.16GlyCys: 1.16 ± 0.295
5.196GlyAsp: 5.196 ± 0.503
5.335GlyGlu: 5.335 ± 0.604
2.041GlyPhe: 2.041 ± 0.385
13.314GlyGly: 13.314 ± 2.855
1.856GlyHis: 1.856 ± 0.351
4.825GlyIle: 4.825 ± 0.432
3.433GlyLys: 3.433 ± 0.456
7.886GlyLeu: 7.886 ± 0.948
2.273GlyMet: 2.273 ± 0.364
2.691GlyAsn: 2.691 ± 0.478
4.407GlyPro: 4.407 ± 0.544
3.433GlyGln: 3.433 ± 0.534
5.892GlyArg: 5.892 ± 0.617
5.103GlySer: 5.103 ± 0.54
6.866GlyThr: 6.866 ± 0.676
6.727GlyVal: 6.727 ± 0.557
1.809GlyTrp: 1.809 ± 0.316
2.598GlyTyr: 2.598 ± 0.356
0.0GlyXaa: 0.0 ± 0.0
His
1.995HisAla: 1.995 ± 0.348
0.232HisCys: 0.232 ± 0.108
1.206HisAsp: 1.206 ± 0.303
1.485HisGlu: 1.485 ± 0.301
0.464HisPhe: 0.464 ± 0.139
1.577HisGly: 1.577 ± 0.258
0.835HisHis: 0.835 ± 0.277
1.438HisIle: 1.438 ± 0.307
0.418HisLys: 0.418 ± 0.173
1.299HisLeu: 1.299 ± 0.289
0.464HisMet: 0.464 ± 0.15
0.418HisAsn: 0.418 ± 0.133
1.206HisPro: 1.206 ± 0.249
0.278HisGln: 0.278 ± 0.083
2.273HisArg: 2.273 ± 0.426
0.928HisSer: 0.928 ± 0.172
1.856HisThr: 1.856 ± 0.305
0.928HisVal: 0.928 ± 0.219
0.186HisTrp: 0.186 ± 0.092
0.557HisTyr: 0.557 ± 0.174
0.0HisXaa: 0.0 ± 0.0
Ile
5.799IleAla: 5.799 ± 0.453
0.557IleCys: 0.557 ± 0.145
3.943IleAsp: 3.943 ± 0.4
4.685IleGlu: 4.685 ± 0.494
0.881IlePhe: 0.881 ± 0.18
5.196IleGly: 5.196 ± 0.505
0.742IleHis: 0.742 ± 0.204
1.67IleIle: 1.67 ± 0.338
1.438IleLys: 1.438 ± 0.282
2.783IleLeu: 2.783 ± 0.341
0.881IleMet: 0.881 ± 0.212
1.345IleAsn: 1.345 ± 0.264
2.644IlePro: 2.644 ± 0.329
0.881IleGln: 0.881 ± 0.234
2.18IleArg: 2.18 ± 0.359
2.505IleSer: 2.505 ± 0.351
3.387IleThr: 3.387 ± 0.358
3.108IleVal: 3.108 ± 0.394
0.278IleTrp: 0.278 ± 0.106
0.928IleTyr: 0.928 ± 0.18
0.0IleXaa: 0.0 ± 0.0
Lys
4.268LysAla: 4.268 ± 0.539
0.139LysCys: 0.139 ± 0.081
1.485LysAsp: 1.485 ± 0.216
0.696LysGlu: 0.696 ± 0.267
0.649LysPhe: 0.649 ± 0.156
2.691LysGly: 2.691 ± 0.336
0.51LysHis: 0.51 ± 0.162
1.577LysIle: 1.577 ± 0.27
1.021LysLys: 1.021 ± 0.288
2.923LysLeu: 2.923 ± 0.372
0.418LysMet: 0.418 ± 0.133
0.742LysAsn: 0.742 ± 0.196
1.299LysPro: 1.299 ± 0.303
1.438LysGln: 1.438 ± 0.242
2.041LysArg: 2.041 ± 0.294
1.253LysSer: 1.253 ± 0.219
2.32LysThr: 2.32 ± 0.32
2.783LysVal: 2.783 ± 0.338
0.371LysTrp: 0.371 ± 0.106
0.603LysTyr: 0.603 ± 0.17
0.0LysXaa: 0.0 ± 0.0
Leu
11.644LeuAla: 11.644 ± 0.884
0.974LeuCys: 0.974 ± 0.25
6.263LeuAsp: 6.263 ± 0.693
3.201LeuGlu: 3.201 ± 0.355
1.902LeuPhe: 1.902 ± 0.366
7.33LeuGly: 7.33 ± 1.075
1.021LeuHis: 1.021 ± 0.201
3.758LeuIle: 3.758 ± 0.461
2.412LeuLys: 2.412 ± 0.4
5.289LeuLeu: 5.289 ± 0.615
1.67LeuMet: 1.67 ± 0.25
3.247LeuAsn: 3.247 ± 0.599
5.892LeuPro: 5.892 ± 0.528
1.577LeuGln: 1.577 ± 0.265
4.222LeuArg: 4.222 ± 0.485
4.082LeuSer: 4.082 ± 0.407
7.144LeuThr: 7.144 ± 0.629
4.964LeuVal: 4.964 ± 0.467
1.206LeuTrp: 1.206 ± 0.256
1.716LeuTyr: 1.716 ± 0.333
0.0LeuXaa: 0.0 ± 0.0
Met
2.412MetAla: 2.412 ± 0.328
0.278MetCys: 0.278 ± 0.108
0.603MetAsp: 0.603 ± 0.159
0.696MetGlu: 0.696 ± 0.158
0.835MetPhe: 0.835 ± 0.197
1.392MetGly: 1.392 ± 0.214
0.418MetHis: 0.418 ± 0.162
0.881MetIle: 0.881 ± 0.195
0.649MetLys: 0.649 ± 0.148
1.392MetLeu: 1.392 ± 0.222
0.464MetMet: 0.464 ± 0.15
0.464MetAsn: 0.464 ± 0.153
1.485MetPro: 1.485 ± 0.304
0.371MetGln: 0.371 ± 0.141
1.485MetArg: 1.485 ± 0.254
1.995MetSer: 1.995 ± 0.283
2.783MetThr: 2.783 ± 0.354
1.392MetVal: 1.392 ± 0.219
0.649MetTrp: 0.649 ± 0.169
0.51MetTyr: 0.51 ± 0.142
0.0MetXaa: 0.0 ± 0.0
Asn
3.34AsnAla: 3.34 ± 0.416
0.232AsnCys: 0.232 ± 0.112
1.809AsnAsp: 1.809 ± 0.358
1.253AsnGlu: 1.253 ± 0.217
0.325AsnPhe: 0.325 ± 0.118
3.433AsnGly: 3.433 ± 0.449
0.603AsnHis: 0.603 ± 0.15
1.345AsnIle: 1.345 ± 0.321
0.974AsnLys: 0.974 ± 0.275
1.948AsnLeu: 1.948 ± 0.297
0.464AsnMet: 0.464 ± 0.101
1.206AsnAsn: 1.206 ± 0.296
1.902AsnPro: 1.902 ± 0.324
0.464AsnGln: 0.464 ± 0.129
2.041AsnArg: 2.041 ± 0.356
1.577AsnSer: 1.577 ± 0.292
2.551AsnThr: 2.551 ± 0.426
1.902AsnVal: 1.902 ± 0.287
0.371AsnTrp: 0.371 ± 0.16
0.881AsnTyr: 0.881 ± 0.183
0.0AsnXaa: 0.0 ± 0.0
Pro
7.469ProAla: 7.469 ± 0.633
0.325ProCys: 0.325 ± 0.129
4.314ProAsp: 4.314 ± 0.53
5.289ProGlu: 5.289 ± 0.656
1.253ProPhe: 1.253 ± 0.228
5.984ProGly: 5.984 ± 0.724
1.299ProHis: 1.299 ± 0.278
2.459ProIle: 2.459 ± 0.41
1.113ProLys: 1.113 ± 0.21
3.479ProLeu: 3.479 ± 0.361
1.206ProMet: 1.206 ± 0.188
1.995ProAsn: 1.995 ± 0.245
4.685ProPro: 4.685 ± 0.66
1.809ProGln: 1.809 ± 0.269
3.85ProArg: 3.85 ± 0.534
2.366ProSer: 2.366 ± 0.392
4.175ProThr: 4.175 ± 0.382
5.057ProVal: 5.057 ± 0.454
1.345ProTrp: 1.345 ± 0.211
1.067ProTyr: 1.067 ± 0.239
0.0ProXaa: 0.0 ± 0.0
Gln
4.129GlnAla: 4.129 ± 0.676
0.325GlnCys: 0.325 ± 0.129
1.067GlnAsp: 1.067 ± 0.245
0.881GlnGlu: 0.881 ± 0.205
1.021GlnPhe: 1.021 ± 0.208
2.18GlnGly: 2.18 ± 0.367
0.835GlnHis: 0.835 ± 0.186
2.134GlnIle: 2.134 ± 0.295
0.789GlnLys: 0.789 ± 0.243
2.412GlnLeu: 2.412 ± 0.273
0.603GlnMet: 0.603 ± 0.142
0.696GlnAsn: 0.696 ± 0.185
1.67GlnPro: 1.67 ± 0.258
1.716GlnGln: 1.716 ± 0.237
3.155GlnArg: 3.155 ± 0.496
1.67GlnSer: 1.67 ± 0.312
2.273GlnThr: 2.273 ± 0.301
1.995GlnVal: 1.995 ± 0.279
0.649GlnTrp: 0.649 ± 0.183
0.696GlnTyr: 0.696 ± 0.194
0.0GlnXaa: 0.0 ± 0.0
Arg
7.376ArgAla: 7.376 ± 0.641
0.557ArgCys: 0.557 ± 0.163
4.454ArgAsp: 4.454 ± 0.495
5.149ArgGlu: 5.149 ± 0.746
1.392ArgPhe: 1.392 ± 0.248
4.268ArgGly: 4.268 ± 0.53
1.856ArgHis: 1.856 ± 0.351
2.598ArgIle: 2.598 ± 0.324
2.041ArgLys: 2.041 ± 0.343
5.984ArgLeu: 5.984 ± 0.5
1.577ArgMet: 1.577 ± 0.336
2.32ArgAsn: 2.32 ± 0.344
4.129ArgPro: 4.129 ± 0.602
2.644ArgGln: 2.644 ± 0.37
6.634ArgArg: 6.634 ± 0.761
2.923ArgSer: 2.923 ± 0.355
4.036ArgThr: 4.036 ± 0.46
5.381ArgVal: 5.381 ± 0.645
1.809ArgTrp: 1.809 ± 0.306
2.366ArgTyr: 2.366 ± 0.393
0.0ArgXaa: 0.0 ± 0.0
Ser
5.057SerAla: 5.057 ± 0.545
0.557SerCys: 0.557 ± 0.155
3.479SerAsp: 3.479 ± 0.359
2.18SerGlu: 2.18 ± 0.336
0.696SerPhe: 0.696 ± 0.212
5.938SerGly: 5.938 ± 0.817
0.789SerHis: 0.789 ± 0.225
1.67SerIle: 1.67 ± 0.311
1.253SerLys: 1.253 ± 0.229
4.222SerLeu: 4.222 ± 0.651
1.299SerMet: 1.299 ± 0.221
1.067SerAsn: 1.067 ± 0.23
3.155SerPro: 3.155 ± 0.378
1.948SerGln: 1.948 ± 0.258
3.433SerArg: 3.433 ± 0.32
2.505SerSer: 2.505 ± 0.393
3.897SerThr: 3.897 ± 0.49
3.897SerVal: 3.897 ± 0.46
1.067SerTrp: 1.067 ± 0.196
1.438SerTyr: 1.438 ± 0.225
0.0SerXaa: 0.0 ± 0.0
Thr
8.861ThrAla: 8.861 ± 0.635
0.418ThrCys: 0.418 ± 0.136
5.428ThrAsp: 5.428 ± 0.463
3.201ThrGlu: 3.201 ± 0.419
1.577ThrPhe: 1.577 ± 0.264
7.005ThrGly: 7.005 ± 0.501
1.16ThrHis: 1.16 ± 0.235
3.665ThrIle: 3.665 ± 0.378
2.412ThrLys: 2.412 ± 0.302
5.381ThrLeu: 5.381 ± 0.53
1.809ThrMet: 1.809 ± 0.25
2.041ThrAsn: 2.041 ± 0.428
5.01ThrPro: 5.01 ± 0.585
1.856ThrGln: 1.856 ± 0.302
4.082ThrArg: 4.082 ± 0.485
4.222ThrSer: 4.222 ± 0.384
3.804ThrThr: 3.804 ± 0.49
6.031ThrVal: 6.031 ± 0.647
1.253ThrTrp: 1.253 ± 0.263
1.809ThrTyr: 1.809 ± 0.293
0.0ThrXaa: 0.0 ± 0.0
Val
8.35ValAla: 8.35 ± 0.485
0.881ValCys: 0.881 ± 0.198
4.454ValAsp: 4.454 ± 0.445
5.613ValGlu: 5.613 ± 0.613
1.531ValPhe: 1.531 ± 0.243
7.469ValGly: 7.469 ± 0.651
1.624ValHis: 1.624 ± 0.263
3.526ValIle: 3.526 ± 0.334
1.856ValLys: 1.856 ± 0.309
6.541ValLeu: 6.541 ± 0.393
1.392ValMet: 1.392 ± 0.281
2.134ValAsn: 2.134 ± 0.284
3.897ValPro: 3.897 ± 0.325
1.856ValGln: 1.856 ± 0.24
4.825ValArg: 4.825 ± 0.555
3.99ValSer: 3.99 ± 0.386
5.289ValThr: 5.289 ± 0.498
6.031ValVal: 6.031 ± 0.623
1.902ValTrp: 1.902 ± 0.32
1.995ValTyr: 1.995 ± 0.364
0.0ValXaa: 0.0 ± 0.0
Trp
2.366TrpAla: 2.366 ± 0.385
0.418TrpCys: 0.418 ± 0.167
1.253TrpAsp: 1.253 ± 0.242
0.51TrpGlu: 0.51 ± 0.139
0.696TrpPhe: 0.696 ± 0.225
1.113TrpGly: 1.113 ± 0.216
0.464TrpHis: 0.464 ± 0.162
0.928TrpIle: 0.928 ± 0.204
0.278TrpLys: 0.278 ± 0.101
1.485TrpLeu: 1.485 ± 0.255
0.464TrpMet: 0.464 ± 0.131
0.278TrpAsn: 0.278 ± 0.095
0.881TrpPro: 0.881 ± 0.195
0.789TrpGln: 0.789 ± 0.193
1.345TrpArg: 1.345 ± 0.189
1.345TrpSer: 1.345 ± 0.262
1.392TrpThr: 1.392 ± 0.288
1.531TrpVal: 1.531 ± 0.335
0.464TrpTrp: 0.464 ± 0.135
0.418TrpTyr: 0.418 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.201TyrAla: 3.201 ± 0.333
0.278TyrCys: 0.278 ± 0.111
2.18TyrAsp: 2.18 ± 0.24
1.485TyrGlu: 1.485 ± 0.288
0.603TyrPhe: 0.603 ± 0.153
2.088TyrGly: 2.088 ± 0.354
0.464TyrHis: 0.464 ± 0.12
1.021TyrIle: 1.021 ± 0.185
0.557TyrLys: 0.557 ± 0.178
2.041TyrLeu: 2.041 ± 0.373
0.418TyrMet: 0.418 ± 0.152
0.649TyrAsn: 0.649 ± 0.152
1.16TyrPro: 1.16 ± 0.24
0.928TyrGln: 0.928 ± 0.215
2.32TyrArg: 2.32 ± 0.346
1.067TyrSer: 1.067 ± 0.195
1.624TyrThr: 1.624 ± 0.244
2.18TyrVal: 2.18 ± 0.222
0.278TyrTrp: 0.278 ± 0.112
0.603TyrTyr: 0.603 ± 0.167
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 99 proteins (21557 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski