Streptococcus satellite phage Javan495

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZQ36|A0A4D5ZQ36_9VIRU Putative DNA-binding phage protein OS=Streptococcus satellite phage Javan495 OX=2558724 GN=JavanS495_0004 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84TFSDD6 pKa = 3.67TPKK9 pKa = 10.21TFTFHH14 pKa = 5.7YY15 pKa = 8.55TFKK18 pKa = 11.1DD19 pKa = 3.09FDD21 pKa = 4.09TAQVACHH28 pKa = 6.88AILGYY33 pKa = 7.77MTGTYY38 pKa = 7.11EE39 pKa = 4.76QPVIDD44 pKa = 3.97ATYY47 pKa = 10.82HH48 pKa = 6.24NDD50 pKa = 3.59DD51 pKa = 3.72QGGHH55 pKa = 6.63ANQLVLKK62 pKa = 9.52YY63 pKa = 10.97AEE65 pKa = 4.31DD66 pKa = 3.69RR67 pKa = 11.84KK68 pKa = 10.48LSKK71 pKa = 10.32VFKK74 pKa = 10.15RR75 pKa = 11.84ICDD78 pKa = 3.69SFKK81 pKa = 10.93DD82 pKa = 4.21YY83 pKa = 11.52YY84 pKa = 10.51NQPEE88 pKa = 4.58DD89 pKa = 3.61MTDD92 pKa = 4.14DD93 pKa = 4.37EE94 pKa = 6.25LDD96 pKa = 4.42DD97 pKa = 3.69MAQEE101 pKa = 4.09NEE103 pKa = 4.29LIKK106 pKa = 10.68EE107 pKa = 4.0IEE109 pKa = 4.37EE110 pKa = 4.41PNHH113 pKa = 5.12QRR115 pKa = 11.84VVSLSKK121 pKa = 9.75STQGKK126 pKa = 10.1VNDD129 pKa = 3.94QDD131 pKa = 3.49TFMTFISDD139 pKa = 3.61HH140 pKa = 5.84NQLAEE145 pKa = 4.25HH146 pKa = 6.91LSMNYY151 pKa = 10.19KK152 pKa = 10.52EE153 pKa = 4.5MTPEE157 pKa = 3.92DD158 pKa = 3.6LGAILEE164 pKa = 4.71SISQAFNHH172 pKa = 6.73LYY174 pKa = 11.22DD175 pKa = 3.83MVVEE179 pKa = 4.32GQLLVKK185 pKa = 10.69

Molecular weight: 21.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZRJ7|A0A4D5ZRJ7_9VIRU DNA primase OS=Streptococcus satellite phage Javan495 OX=2558724 GN=JavanS495_0012 PE=4 SV=1
MM1 pKa = 7.31IMKK4 pKa = 8.92ITEE7 pKa = 4.19HH8 pKa = 6.36KK9 pKa = 10.01KK10 pKa = 10.26KK11 pKa = 10.5NDD13 pKa = 3.87TIVYY17 pKa = 8.88RR18 pKa = 11.84ASIYY22 pKa = 10.8LGIDD26 pKa = 2.83QMTGKK31 pKa = 10.05RR32 pKa = 11.84VKK34 pKa = 9.97TSITGRR40 pKa = 11.84TRR42 pKa = 11.84KK43 pKa = 9.11EE44 pKa = 3.7VNQKK48 pKa = 10.3AKK50 pKa = 10.27HH51 pKa = 5.39AQLDD55 pKa = 3.99FLSNGSTIKK64 pKa = 10.42RR65 pKa = 11.84KK66 pKa = 10.42YY67 pKa = 9.8EE68 pKa = 3.88GG69 pKa = 3.44

Molecular weight: 7.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19

0

19

3225

57

500

169.7

19.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.457 ± 0.364

0.837 ± 0.222

5.457 ± 0.365

8.155 ± 0.515

4.248 ± 0.275

4.651 ± 0.501

1.86 ± 0.303

7.163 ± 0.577

8.93 ± 0.325

9.519 ± 0.682

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.922 ± 0.231

5.736 ± 0.412

3.349 ± 0.662

4.496 ± 0.342

5.302 ± 0.366

5.395 ± 0.398

6.14 ± 0.328

5.209 ± 0.238

1.116 ± 0.284

5.054 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski