Carnation ringspot virus (isolate Lommel) (CRSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Dianthovirus; Carnation ringspot virus

Average proteome isoelectric point is 8.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q66096-2|RDRP-2_CRSVL Isoform of Q66096 Isoform protein p27 of RNA-directed RNA polymerase OS=Carnation ringspot virus (isolate Lommel) OX=652597 GN=ORF1 PE=4 SV=2
MM1 pKa = 7.86AIFEE5 pKa = 4.99LFSFDD10 pKa = 4.03IDD12 pKa = 3.99KK13 pKa = 11.23LLVWVSKK20 pKa = 9.79FSPGKK25 pKa = 9.66ILSAICRR32 pKa = 11.84LGLDD36 pKa = 2.89SWNNFRR42 pKa = 11.84KK43 pKa = 9.4WFWGLNFDD51 pKa = 3.5KK52 pKa = 11.23HH53 pKa = 5.07EE54 pKa = 4.07WAVDD58 pKa = 3.09HH59 pKa = 6.77FMPLMPRR66 pKa = 11.84FSSDD70 pKa = 2.55MDD72 pKa = 3.51EE73 pKa = 4.03VAKK76 pKa = 10.78RR77 pKa = 11.84IVTPKK82 pKa = 10.39SKK84 pKa = 10.54PKK86 pKa = 10.92LEE88 pKa = 4.78DD89 pKa = 3.35CLEE92 pKa = 4.08IDD94 pKa = 3.97TAVEE98 pKa = 3.86EE99 pKa = 4.83CFNEE103 pKa = 4.37EE104 pKa = 3.89CFEE107 pKa = 4.11PQEE110 pKa = 4.54DD111 pKa = 3.81GSMKK115 pKa = 10.19LKK117 pKa = 10.41RR118 pKa = 11.84VAAPQQIKK126 pKa = 9.94RR127 pKa = 11.84VRR129 pKa = 11.84TGMIEE134 pKa = 3.95DD135 pKa = 5.31AISAVEE141 pKa = 3.48ARR143 pKa = 11.84IRR145 pKa = 11.84NRR147 pKa = 11.84HH148 pKa = 5.74MIMGDD153 pKa = 3.29DD154 pKa = 3.49MGLVDD159 pKa = 4.55EE160 pKa = 4.8AAVRR164 pKa = 11.84ATAMDD169 pKa = 2.88ICGEE173 pKa = 4.11YY174 pKa = 10.14KK175 pKa = 10.25INEE178 pKa = 3.88HH179 pKa = 5.62HH180 pKa = 6.47TRR182 pKa = 11.84CIIYY186 pKa = 9.64AAAYY190 pKa = 10.02RR191 pKa = 11.84AMTPDD196 pKa = 3.43QEE198 pKa = 5.72SIDD201 pKa = 4.03ATKK204 pKa = 9.72MAYY207 pKa = 9.67NPKK210 pKa = 9.32SQARR214 pKa = 11.84RR215 pKa = 11.84DD216 pKa = 3.6LVSILRR222 pKa = 11.84KK223 pKa = 9.94NISFGGFKK231 pKa = 10.66SLEE234 pKa = 4.18DD235 pKa = 3.89FLNN238 pKa = 3.66

Molecular weight: 27.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q66096-2|RDRP-2_CRSVL Isoform of Q66096 Isoform protein p27 of RNA-directed RNA polymerase OS=Carnation ringspot virus (isolate Lommel) OX=652597 GN=ORF1 PE=4 SV=2
MM1 pKa = 7.43LSSYY5 pKa = 10.43LRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84EE10 pKa = 4.04PTRR13 pKa = 11.84HH14 pKa = 5.95KK15 pKa = 10.76SVIRR19 pKa = 11.84ILRR22 pKa = 11.84NRR24 pKa = 11.84QSATLVGGGRR34 pKa = 11.84RR35 pKa = 11.84VGFSLGVVPKK45 pKa = 10.85DD46 pKa = 4.46NLPTWWRR53 pKa = 11.84HH54 pKa = 3.83QACCGVIEE62 pKa = 4.69SRR64 pKa = 11.84TLVLAVVKK72 pKa = 9.47FVSVLFFCLYY82 pKa = 9.91VGSVCVTRR90 pKa = 11.84TDD92 pKa = 3.65GSRR95 pKa = 11.84KK96 pKa = 8.85PAVKK100 pKa = 10.3

Molecular weight: 11.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

1

5

1756

100

769

351.2

39.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.777 ± 0.552

2.335 ± 0.435

6.15 ± 0.52

4.841 ± 1.11

4.385 ± 0.524

5.41 ± 0.347

1.822 ± 0.264

5.068 ± 0.508

6.492 ± 0.708

7.802 ± 0.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.018 ± 0.425

4.1 ± 0.367

5.011 ± 0.535

3.189 ± 0.386

7.062 ± 0.533

8.599 ± 0.751

5.353 ± 1.388

7.973 ± 1.001

1.595 ± 0.19

3.018 ± 0.38
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski