Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6)

Taxonomy: cellular organisms; Bacteria; Aquificae; Aquificae; Aquificales; Aquificaceae; Hydrogenobacter; Hydrogenobacter thermophilus

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1892 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3DKI4|D3DKI4_HYDTT Succinate--CoA ligase [ADP-forming] subunit alpha OS=Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6) OX=608538 GN=sucD PE=3 SV=1
MM1 pKa = 7.85ALRR4 pKa = 11.84TMVDD8 pKa = 3.21PDD10 pKa = 3.7TCTSCEE16 pKa = 3.86LCYY19 pKa = 11.0DD20 pKa = 4.42RR21 pKa = 11.84VPEE24 pKa = 4.2VYY26 pKa = 10.24KK27 pKa = 11.12NRR29 pKa = 11.84GDD31 pKa = 4.2GIAEE35 pKa = 4.24VVSPGPDD42 pKa = 2.67GWMMVPPEE50 pKa = 4.39LEE52 pKa = 4.04QEE54 pKa = 4.44VKK56 pKa = 10.48EE57 pKa = 4.37VTDD60 pKa = 3.78EE61 pKa = 4.34CPSGSIITEE70 pKa = 4.11EE71 pKa = 4.14VV72 pKa = 2.85

Molecular weight: 7.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3DJ34|D3DJ34_HYDTT Putative Rossmann fold nucleotide-binding protein OS=Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6) OX=608538 GN=HTH_1385 PE=4 SV=1
MM1 pKa = 7.24ATQRR5 pKa = 11.84NITRR9 pKa = 11.84ISNFKK14 pKa = 10.22RR15 pKa = 11.84KK16 pKa = 9.44RR17 pKa = 11.84KK18 pKa = 9.86SGFLARR24 pKa = 11.84MSTKK28 pKa = 10.25SGRR31 pKa = 11.84KK32 pKa = 8.01IINRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 8.85GRR42 pKa = 11.84KK43 pKa = 8.79RR44 pKa = 11.84LAPP47 pKa = 3.99

Molecular weight: 5.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1892

0

1892

555287

37

1566

293.5

33.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.282 ± 0.047

0.998 ± 0.022

4.775 ± 0.036

8.023 ± 0.072

4.749 ± 0.048

6.939 ± 0.052

1.78 ± 0.025

7.021 ± 0.047

7.792 ± 0.061

11.011 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.354 ± 0.024

3.153 ± 0.032

4.038 ± 0.036

2.619 ± 0.031

5.403 ± 0.039

5.894 ± 0.051

4.219 ± 0.035

7.693 ± 0.057

1.028 ± 0.019

4.231 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski